ReadyStrip™ IPG Strip Instruction Manual For technical service call your local Bio-Rad office or in the US call 1-800-4BIORAD (1-800-424-6723)
Introduction Recommended protein load ranges The combination of isoelectric focusing (IEF) with SDS-PAGE provides a powerful technique for the separation of complex mixtures of proteins. ReadyStrip IPG strips are made with buffering acrylamide derivatives that contain either a free carboxylic acid or a tertiary amino group, which is copolymerized with acrylamide and bis-acrylamide. As such, the pH gradient is precast into the gel and cannot shift during electrophoresis.
* The final voltage for each pH range may not be reached, but the total volt-hours given above are sufficient to properly focus samples with final voltages as low as 3,000 V (7 cm), 5,000 V (11 cm), and 7,000 V (17 cm, 18 cm, and 24 cm). A lower final voltage will increase total run time.
Ordering Information Catalog # Catalog # Description Sample Preparation Kits 163-2130 ReadyPrep 2-D Cleanup Kit, 50 preps 163-2089 ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear), 50 preps 163-2088 ReadyPrep Protein Extraction Kit (Membrane 1), 50 preps 163-2087 ReadyPrep Protein Extraction Kit (Signal), 50 preps 163-2086 ReadyPrep Protein Extraction Kit (Total Protein), 20 preps 163-2085 ReadyPrep Protein Extraction Kit (Soluble/Insoluble), 20 preps 163-2084 ReadyPrep Protein Extraction Kit (
