User Manual

Chapter 8: Protease Analysis by Zymogram PAGE .................................... 23
8.1 Introduction .................................................................. 23
8.2 Criterion Zymogram Gels........................................................ 23
8.2.1 Gel Composition .......................................................... 23
8.2.2 Gel Selection Guide........................................................ 23
8.3 Zymogram Buffers............................................................. 23
8.4 Sample Preparation............................................................ 24
8.5 Running Conditions ............................................................ 24
Chapter 9: Nondenaturing Nucleic Acid PAGE ........................................ 25
9.1 Introduction .................................................................. 25
9.2 Criterion TBE Gels............................................................. 25
9.2.1 Gel Composition .......................................................... 25
9.2.2 Gel Selection Guide........................................................ 25
9.3 Nondenaturing Nucleic Acid PAGE Buffers .......................................... 25
9.4 Sample Preparation............................................................ 26
9.5 Running Conditions ............................................................ 26
Chapter 10: Denaturing Nucleic Acid PAGE .......................................... 27
10.1 Introduction ................................................................. 27
10.2 Criterion TBE-Urea Gels ....................................................... 27
10.2.1 Gel Composition ......................................................... 27
10.2.2 Gel Selection Guide....................................................... 27
10.3 TBE-Urea Buffers ............................................................ 27
10.4 Sample Preparation........................................................... 27
10.5 Running Conditions ........................................................... 28
Chapter 11: 2-D Electrophoresis ................................................... 29
11.1 Introduction ................................................................. 29
11.2 Equilibration................................................................. 29
11.3 Agarose Overlay ............................................................. 29
11.4 Second-Dimension Electrophoresis............................................... 29