M1250131B 11/28/2000 3:16 PM Page B Standard Cartridge Holder and Deashing Holder Instruction Manual Bio-Rad Laboratories, 2000 Alfred Nobel Dr.
M1250131B 11/28/2000 3:16 PM Page D Warranty Holders received damaged in shipment or defective in workmanship will be replaced if returned within 60 days of the invoice date. No other warranty as to end use, seal life, or performance is made. Introduction The Standard Cartridge Holder is a convenient, reusable holder that will accommodate any of Bio-Rad’s 30 x 4.6 mm HPLC cartridges. Its function is to connect the cartridge to the HPLC system. The Deashing Holder accommodates two 30 x 4.
M1250131B 11/28/2000 3:16 PM Page 4 must be cut, file or grind the tubing end as necessary so that the end cut is perpendicular to the tubing axis, and so that the internal diameter has not been constricted. Nut Ferrule Holder 1/16" stainless steel tubing Fig. 1. Connections to the holder. Use 1/16" 316 stainless steel tubing, 0.010" ID, to connect the holder. See Figure 1. Tighten the assembly of tubing, ferrule, and nut finger-tight. Be sure the tubing end bottoms squarely in the holder fitting.
M1250131B 11/28/2000 3:16 PM Page 6 When using a buffer as mobile phase, rinse the holder’s end fittings with water after removing the cartridge. This will prevent salt precipitation which would scratch or damage the seals. Specifications Materials in contact with the mobile phase are 316 stainless steel and Kel-F® fluorocarbon.