User Manual

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Aurum

™

Vacuum Manifold

Instruction Manual

For technical support, call your local Bio-Rad office, or

in the US, call 1-800-4BIORAD (1-800-424-6723).

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Life Science

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Bio-Rad

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Summary of content (8 pages)

PAGE 1
Aurum™ Vacuum Manifold Instruction Manual Bio-Rad Laboratories, Inc. Web site www.bio-rad.
PAGE 2
Table of Contents Section 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . .1 Section 2 Kit Components . . . . . . . . . . . . . . . . . . . . . . . . .1 Section 3 Care and Maintenance . . . . . . . . . . . . . . . . . . . .2 Section 4 Aurum™ Vacuum Manifold . . . . . . . . . . . . . . . . .3 Section 5 Vacuum Regulator . . . . . . . . . . . . . . . . . . . . . . .4 Section 6 Vacuum Manifold Setup . . . . . . . . . . . . . . . . . . .5 Section 7 Use for 96-Well Plates . . . .
PAGE 3
Section 1 Introduction The Aurum™ vacuum manifold and column adaptor plate (CAP)` comprise a system which allows a single vacuum manifold to be used with either 96-well plates or columns with luer ends. Separate manifolds are no longer required for different preparative formats; the Aurum vacuum manifold and CAP ensure fast, high-quality sample preparation in both formats, while maintaining the simplicity of handling associated with vacuum processing.
PAGE 4
Section 4 Aurum™ Vacuum Manifold Manifold top A stage Manifold base with quick connect fitting Stage Vacuum regulator Waste collection tray The Aurum vacuum manifold is designed to accommodate the 96-well plates used in Bio-Rad's Aurum total RNA 96 kit. The manifold may also be compatible with other manufacturers' kits. For column processing, the column adaptor plate (CAP) is required. The CAP allows the Aurum vacuum manifold to to be used for processing columns with a luer fitting.
PAGE 5
Section 5 Vacuum Regulator Section 6 Vacuum Manifold Setup The vacuum regulator (see Figure 2) allows complete control of the negative pressures inside the Aurum™ vacuum manifold, allowing optimum levels of negative pressure for efficient sample preparation. This is particularly important when a gradual increase in negative pressure is required during 96-well plate purifications.
PAGE 6
Manifold wash setup (see Figure 4A) Section 8 Use for the Column Adaptor Plate (CAP) 1. Place the manifold top on the base, ensuring complete and uniform contact between the manifold top and base. Manifold wash setup (see Figure 5) 2. Place the desired 96-well purification plate on the manifold top and apply the recommended vacuum pressure for your application. Section 7 Use for 96-Well Plates 1. Insert the CAP (luer ends up) into the depression in the vacuum manifold top.
PAGE 7
Section 9 Troubleshooting Guide Problem Possible Cause Problem Possible Cause Possible Solution Incorrect negative pressure from vacuum regulator Vacuum source connected to left side of vacuum Connect vacuum to right side of vacuum Clogging of purification plate or CAP columns Insufficient negative pressure in vacuum manifold See “Insufficient negative pressure in vacuum manifold” problem above Possible Solution Insufficient negative Open vacuum regulator pressure in vacuum manifold Close vacuu
PAGE 8
Section 10 Ordering Information Catalog # Description 732-6470 Aurum Vacuum Manifold 732-6820 Aurum Total RNA Mini Kit 732-6830 Aurum Total RNA Fatty and Fibrous Tissue Kit 732-6800 Aurum Total RNA 96 Kit 732-6400 Aurum Plasmid Mini Kit 10