Maintenance Kit Owner's manual

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GRADIENT PUMP MAINTENANCE KIT

FOR THE BIOLOGIC SYSTEM

CATALOG NUMBER 750-0164

The BioLogic System Gradient Pump require minimal maintenance to stay in good working condition.

This maintenance kit includes the piston seals, check valves, and piston wipers for routine mainte-

nance of the pump heads.

1. Replacing a piston seal. (Refer to Figure 1.)

a. Disconnect all tubing from the pump head.

b. Using the supplied Allen wrench, remove the four Pump Head Module Assembly screws on

the

face

of the Pump Head Module in the order shown. Gently pull the pump head away

from the Workstation.

Figure 1. BioLogic Workstation Gradient Pump Mechanism Parts

c. Remove the washout block from the back of the pump head module by gently pulling it away.

d. Note that there are two piston seals per pumphead. Always replace both seals at the same

time. Once a piston seal is removed from its gland, it cannot be reused. It is recommended to

pre-condition the new seals by soaking or sonicating them in water for several minutes.

Always shake off or blot excess moisture from the seals before installation.

e. Using the Piston Seal Removal Tool, pry the seal from the gland. To do this, you insert the tip

of the Seal Removal Tool into the seal and gently pry the seal away. (See Figure 2.)

f. To install a new seal, line it up with the gland and use the Washout Block to seat the seal.

g. When both seals have been replaced, reassemble the pump head by reversing the

sequence shown in Figure 1. Firstly, wipe the pistons with a dampened cloth to ensure that

no crystallized salt residues are present which could damage the new seals. Then, position

PUMP HEAD

MODULE

PUMP HEAD

MODULE

ASSEMBLY

SCREWS

WASHOUT

BLOCK

SPRING RETAINER

PISTON WIPER

Bio-Rad Laboratories, 2000 Alfred Nobel, Hercules CA 94547 • Phone (510) 741-1000

Summary of content (4 pages)

PAGE 1
GRADIENT PUMP MAINTENANCE KIT FOR THE BIOLOGIC SYSTEM CATALOG NUMBER 750-0164 The BioLogic System Gradient Pump require minimal maintenance to stay in good working condition. This maintenance kit includes the piston seals, check valves, and piston wipers for routine maintenance of the pump heads. 1. Replacing a piston seal. (Refer to Figure 1.) a. Disconnect all tubing from the pump head. b.
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the Washout Block onto the pistons and set the pump head module back onto the Washout Block on the BioLogic Workstation. (The Washout Block guides the pump head module in the reattachment process and protects the pistons.) h. Replace the four screws until the pump head module is secure on the Workstation but do not overtighten. GLAND BACK OF PUMP HEAD MODULE PISTON SEALS PISTON SEAL REMOVAL TOOL Figure 2. Removing the Piston Seals (view showing the back of the Pump Head Module) 2.
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PUMP HEAD MODULE ASSEMBLY SCREWS OUTLET HEAD PUMPING HEAD CHECK VALVE PRIMING HEAD CHECK VALVE Figure 3. Pump Head Module Assembly 3. Replacing the Piston Wiper Piston wipers are available in the event that they are lost during any of the pump maintenance procedures. If the piston wiper has fallen out of the spring retainer, simply place a new piston wiper onto the piston which then rests on the spring retainer. See Figure 1. 4. Plumbing the Gradient Pump a.
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Storage of the Workstation If the BioLogic System will be stored for a long period, be sure to remove all traces of buffer salts from the Workstation pump heads, valves and monitors by flushing extensively with deionized water followed by a 20 % solution of ethanol to prevent microbial growth. Technical Assistance For additional help, contact your local Bio-Rad representative. In the United States, call Technical Service at 1-800-4BIORAD.