Owner manual

ManualsBrandsBio-Rad ManualsMeasuring instrumentsBioLogic DuoFlow™ pH Monitor

The BioLogic pH monitor is an in-line electrode and flow cell that provides direct monitoring of pH conditions during

a sample run. It provides real-time monitoring of buffer pH and is designed for flow through pH recordings. The pH

monitor is available as an option to the BioLogic DuoFlow systems and is compatible with the discontinued

BioLogic HR systems. The monitor ships with all Maximizer systems.

The monitor is supplied with a tubing kit that includes installed ¼ -28 fittings. When used with either a DuoFlow or

HR system the pH monitor connects via the Signal Import Module (SIM). When a Maximizer is installed with the

DuoFlow Workstation the monitor connects directly to the system via a BNC port on the Maximizer.

The pH electrode is a sealed Calomel combination electrode consisting of a pH and a reference electrode built into

a single body. The sealed reference design eliminates the need to add electrolyte solutions and minimizes

reference dry out. The pH electrode is fully compatible with buffers (such as Tris) that may not be compatible with

Ag/AgCl containing electrodes. The pH Monitor is designed for use in chromatography applications at flow rates up

to 80 ml/min and a flow cell pressure less than 75 psi.

The flow cell is made of PEEK and has a swept volume of approximately 80ul when the pH electrode is inserted.

These instructions cover the installation, calibration, and maintenance of the pH electrode and flow cell. Be sure to

keep this information with your BioLogic DuoFlow or HR instruction manual for future reference.

Installation Instructions

Figure 1. pH Electrode and Flow Cell

The flow cell and pH electrode should be plumbed downstream of any backpressure device or detector. The pH

Monitor has a maximum pressure rating of 75 psi.

DuoFlow pH Monitor

Catalog Number 760-2040

Summary of content (4 pages)

PAGE 1
DuoFlow pH Monitor Catalog Number 760-2040 The BioLogic pH monitor is an in-line electrode and flow cell that provides direct monitoring of pH conditions during a sample run. It provides real-time monitoring of buffer pH and is designed for flow through pH recordings. The pH monitor is available as an option to the BioLogic DuoFlow systems and is compatible with the discontinued BioLogic HR systems. The monitor ships with all Maximizer systems.
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Instructions for first time use During shipment, the air bubble in the electrode’s stem may have moved into the bulb area. If bubbles are seen in the bulb area, hold the electrode by its top cap and shake downwards, as is done with a clinical thermometer. Do not expose the electrode to extreme pH solutions for prolonged periods of time, doing so will severely shorten the lifespan of the electrode.
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WORKSTATION MAXIMIZER pH ELECTRODE pH FLOW CELL pH MONITOR Figure 3. pH Monitor connected via a DuoFlow Maximizer. Programming your pH Monitor See the DuoFlow instruction manual for instructions on programming your pH Monitor. Calibrating your pH Monitor The frequency of calibration is a function of the electrode and the solutions it is exposed to. The pH electrode calibration should be checked daily to assure that its accuracy remains within the range required for your application.
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Electrode Cleaning A dirty pH electrode can lead to erroneous pH readings and shorten the lifetime of the electrode. Most contaminant’s will be either proteins that have adsorbed onto the glass membrane or buffer and salt crystals that have blocked the pH probe pores. Removal of protein deposits: Immerse the electrode in a 1% solution of proteases, such as trypsin or pepsin, in 0.1M HCl for no more than 5 minutes. Rinse with deionized water to remove all traces of HCl.