User Manual

Figure 2-10. Run Screen, Showing Chromatogram for QuadTec Detector
The Run screen provides:
• Wavelength status: The wavelengths in use by the QuadTec. The AU output for each wavelength
also is shown.
• QuadTec zero baseline button: Resets the detector AU value to zero. Note: Use care when using
this feature during a collection scheme which uses a Threshold value.
• Chromatogram traces: Each trace can be individually scaled. Use the boxes on each side of the
chromatogram to select a trace. Use the Up/Down scrolling bars on each side of the chromatogram
to scale the trace.
USE WITH THE BIOLOGIC DUO-FLOW SYSTEMSYSTEM INSTALLATION AND SETUP
2-9
Advance
Frac. Collector
Divert Valve
0.0400
50
100% Buffer B
Run Time Run Volume
0.1 ml
WL1 - 280nm
0.40
WL2 - 260nm
0.15
WL3 - 214nm
2.00
WL4 - 405nm
0.00
0:24.9 Port 1: Aux Pump Inlet; Sample 1
Flow Rate EGP %B
0 %B0.00 ml/min 0%
Split
0:00.1
1.00ml/min 0 %B
Gradient Pump: F-10 UV Conductivity
UV Detector
Set
UV Range
Zero
Baseline
Event
mark
Cond Range
500
Protocol: > 1 0:00.0 Isocratic Flow with 100% Buffer B at 1.00 ml/min for 25.0 min
00:00:00 00:02:00 00:04:00 00:06:00
StepTime Left Valve Info
438 psi 1.003 AU 1.23 mS/cm
Minutes
AU
mS/cm
Collect
Waste
View Utilities Options
Edit
Method
New
Method
New
Run Manual Setup
Protocol
wash
load
1
2
Run
Notes PauseAbort Hold
Report
PostRun Full View
Log
Help
Grad. Pump
Chart Recorder
1000
High psi
0
Low psi
QuadTec
QuadTec
Econo Gradient
Pump
Zero
Baseline
0.01
0.0350
0.0300
0.0250
0.0200
0.0150
0.0100
0.0050
0.0000
-0.0050
0.0
3500.0
4000.0
3000.0
2500.0
2000.0
1500.0
1000.0
500.0
-500.0
Fractions
Fraction Vol. Left
SIM1/pHSIM1/SIG
0.548 Volt 7.00 pH
File
Browser
ConductivityQuadTec-1 (280 nm)
Window
BioLogic Duo-Flow - <user name> - <project name> - <method name> - <run name>