User Manual

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AUTO CALIBRATION This procedure searches automatically for reference points within the spectra of the

lamp for reliability and reproducibility of the wavelength. The points used for

calibration are the zero order reflection of the grating (000 nm) and the Ha spectral

line of deuterium (656 nm). Auto calibration is performed automatically when power

to the detector is switched on. To access auto calibration, from the Time Constant

parameter press the Right arrow key to move to this field. Pressing the Up arrow key

starts the procedure for calibration of the wavelength scale.

LAMP The lamp type is specified using the Up arrow to toggle between deuterium and

halogen. Changing the lamp type prompts a question on whether to reset the lamp’s

working time. Pressing any green arrow key will reset the time. The lamp number is

incremented automatically and cannot be edited.

Note: See the instructions on page 5-4 for configuring the appropriate wavelength

settings in the Setup menu after a lamp change.

When replacing a deuterium lamp, the working time should be reset, as this value is

used in the GLP report. Position the cursor on the working time value and press the

Up arrow. Pressing the Up arrow again resets the working time to zero and

increments the lamp number.

The ON/OFF field enables you to switch off the lamp without switching off the

instrument.

Figure 4-3. Lamp Specification Menu

SIGNAL You can select the signal source used for all data outputs using the Up/Down arrow

keys to toggle between the following: Absorption, Signal, and Reference channels.

The values “sig” and “ref” give the measured light intensity on the signal and the

reference diode respectively. The sig and ref values range between 0 and 1. Their

absolute values are dependent on the wavelength, solvent, flow cell used, and age

of the lamp.

With the dummy flow cell in place and a wavelength selection of 240 nm, the value

of reference should be between 0.1 and 0.9. A value of <0.2 indicates that the

deuterium lamp should be replaced, especially if noise and sensitivity are

unacceptable.

SCAN OUT The output speed of scan data via the integrator output. Available values are 1, 2, 5,

10, 20, and 50 steps per second (approximately. 1–50 nm/second).

USE AS A STAND-ALONE SYSTEM SYSTEM INSTALLATION AND SETUP

4-3

Link

View

Setup

Load

Prog

Hold 0

Run 1

Time

(min)

(nm)

Edit Prog

Clear 0

Events:

off 0

on /

Signal 1

[ au ]

Signal 2

[ au ]

GLP