Manual

2.5 DETECTION SYSTEMS
The DuoFlow system supports the following detection and monitoring devices:
• UV Detector
• Conductivity Monitor
• QuadTec UV/VIS Detector
• pH Monitor
2.5.1 UV Detector
The UV detector is a single beam, fixed wavelength UV absorbance detector specifically designed for high
resolution protein chromatography.
• Available in several configurations.
• Rack mountable and portable, which enables it to be positioned close to a column outlet for better
resolution and decreased peak band broadening.
• Optional 214 nm filter and zinc lamp available for sensitive peptide analysis.
Figure 2-5. UV Detector, with Mercury Lamp, 254 & 280 nm Filters, and Conductivity Flow Cell
The UV detector consists of the optics bench, a filter tray, a flow cell, and a lamp. It is designed to hold the
Conductivity monitor flow cell. The following configurations are available:
• Mercury Lamp and Filters. The mercury lamp comes installed in the optics module along with 280
nm and 254 nm filters. The filters are both held by a single tray. To switch filters, rotate the filter
holder. Filters for different wavelengths are also available from Bio-Rad. These include 365 nm, 405
nm, 436 nm, and 546 nm filters.
• Zinc Lamp and 214 nm Filter. The zinc lamp and 214 nm filter are optional. The zinc lamp attaches
directly to the optics module in place of the mercury lamp. (Refer to Figure 2-6)
DESCRIPTION OF BIOLOGIC DUOFLOW SYSTEMSYSTEM OVERVIEW
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