Manual

2.8 SAMPLE LOADING OPTIONS

The BioLogic DuoFlow system supports several methods for sample loading. Typically a sample is loaded

through a static fixed volume loop on the AVR7-3 inject valve. When large volume sample injections are

required, the following options are available:

• Sample loading through the Workstation pumps: The SV5-4, SV3-2 or AVR9-8 valve may be used to

load large sample volumes through the Workstation pumps. Refer to Sections 2.62 through 2.64 for

the discussion of these valves.

• Sample loading through a DynaLoop sliding-piston loop: When used with an AVR7-3 inject valve, the

DynaLoop may be used for large volume sample loading or repetitive injections of smaller volumes

of sample. The DynaLoop comes in two sizes: 25 and 90 ml volume.

• Sample loading through an auxiliary pump, such as the EP-1 Econo pump (EP-1) or the Econo

Gradient Pump (EGP). When used with an AVR7-3 inject valve, an EP-1 or EGP pump may be used

for large volume sample injections. Placing an SV5-4 or AVR9-8 valve before the pump inlet allows

loading of multiple samples.

2.8.1 DynaLoop Large Volume Sample Injection Loop

The DynaLoop, available in 25 ml and 90 ml sizes, is a sliding-piston dynamic loop for injection of a large

sample volume or repetitive injections of smaller samples. The DynaLoop has a sliding piston that functions

like a syringe. The loop is installed on the AVR7-3 in place of the static loop (see Figure 2-23). Sample is

loaded into the DynaLoop’s sample end connector through port #2 on the AVR7-3 valve and may be loaded

with a syringe or auxiliary pumps (such as the Bio-Rad Econo Gradient Pump or EP-1 pump). The loading of

the sample pushes the DynaLoop’s sliding seal towards the buffer end connector. As solution flows into the

buffer end of the DynaLoop it pushes the sample into the system.

Figure 2-22. DynaLoop

0 5 10 15 20 25

30 35 40 45 50 55 60 65 70 75 80 85 90

SAMPLE END

PORT 6 ON AVR 7-3

SLIDING SEAL

ASSEMBLY

BUFFER END

PORT 3 ON AVR 7-3

DESCRIPTION OF SYSTEM COMPONENTS SYSTEM OVERVIEW

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