ProteinChip H4 Array (Reverse Phase) ® Instruction Manual Catalog #C57-30028 For technical support, call your local Bio-Rad office, or in the US, call 1-800-4BIORAD (1-800-424-6723).
Uses n Peptide analysis n Protein profiling and biomarker discovery n Rapid protein analysis to determine purity, mass confirmation, or both How It Works The ProteinChip H4 array binds proteins by reverse-phase interactions. The active surface contains chains of 16 methylene groups that can bind proteins through reverse-phase chemistry via alanine, valine, leucine, isoleucine, phenylalanine, tryptophan, or tyrosine.
Fig. 1. ProteinChip cassette and reservoir. Fig. 2. Removal of ProteinChip arrays from cassette using array forceps. Technical Considerations n Increasing the concentration of organic solvent in the binding and washing solution will increase the selectivity of the surface (only the most hydrophobic proteins will be retained with higher organic solvent concentrations).
Related Products The ProteinChip H50 array (catalog #C53-70065) is an advanced array for reverse-phase retentate chromatography*. The array shows high reproducibility and has a hydrophobic barrier coating for sample containment. The array is recommended for: n n Protein profiling and biomarker discovery Rapid protein analysis to determine purity, mass confirmation, or both Note: We do not recommend the ProteinChip H50 array for peptide analysis.
Protocol 1: Profiling Using the Bioprocessor Note: These protocols are intended as a guideline; you may need to optimize the method for your particular sample type and experimental design. Note: This protocol uses the ProteinChip cassette-compatible bioprocessor; for processing a single array, use an 8-well bioprocessor (catalog #C50-30008). 1. Place the ProteinChip array cassette in the bioprocessor and prewash the ProteinChip arrays by adding 50 µl methanol or acetonitrile for five minutes. Repeat once.
Protocol 2: Profiling On-Spot 1. Outline each spot using a hydrophobic pen. 2. Prewet the spots with 5 µl of binding buffer for 2 minutes. Repeat once. 3. Remove the prewetting solution and replace with 5 µl of sample. Do not allow the spot to air-dry during sample application. 4. Incubate in a humid chamber for 30 minutes with shaking (on MicroMix shaker setting 20/4). 5. Wash each spot with 5 µl binding solution for 2 minutes with shaking, and remove buffer. Repeat two more times. 6.
Ordering Information Catalog # Description C57-30028 C57-30065 C57-30081 C50-30008 C50-30011 ProteinChip H4 Arrays, A–H format, 12 ProteinChip H50 Arrays, A–H format, 12 ProteinChip SEND ID Arrays, A–H format, 12 ProteinChip 8-Well Bioprocessor, A–H format ProteinChip Cassette-Compatible Bioprocessor, includes ProteinChip array forceps, cassette hold-down frame, 12 blank ProteinChip arrays ProteinChip Cassette-Compatible Bioprocessor Reservoirs, 5 ProteinChip Array Forceps, 1 pair ProteinChip CHCA Energy
MicroMix is a trademark of Diagnostic Products Corporation. The SELDI process is covered by US patents 5,719,060, 6,225,047, 6,579,719, 6,818,411, and other issued patents and pending applications in the US and other jurisdictions. Bio-Rad Laboratories, Inc. Web site www.bio-rad.
