ProteinChip NP20 Array (Normal Phase) ® Instruction Manual Catalog #C57-30043 For technical support, call your local Bio-Rad office, or in the US, call 1-800-4BIORAD (1-800-424-6723).
Uses n Binding of highly water-soluble proteins n On-chip sample cleanup n Calibration of the ProteinChip SELDI system instrument using peptides or proteins of known molecular weight How It Works Silicon oxide groups on the active spots of the ProteinChip NP20 array allow proteins to bind through hydrophilic and charged residues, including serine, threonine, and lysine. This surface is commonly used for microscale sample cleanup. Washing with water removes buffer components such as salts and detergents.
Technical Considerations n Prerinsing the ProteinChip NP20 array with HPLC or better grade water may improve results Protocol: On-Spot Note: This protocol is intended as a guideline; you may need to optimize the method for your particular sample type and experimental design. 1. Dilute or dissolve the sample in binding buffer. Most buffers are tolerated by this surface. 2. Load 5 µl of sample per spot (ideally 200–2,000 nM, 10-fold less for peptides). 3.
Ordering Information Catalog # Description C57-30043 C50-30011 ProteinChip NP20 Arrays, A–H format, 12 ProteinChip Cassette-Compatible Bioprocessor, includes ProteinChip array forceps, cassette hold-down frame, 12 blank ProteinChip arrays ProteinChip 8-Well Bioprocessor, A–H format ProteinChip Cassette-Compatible Bioprocessor Reservoirs, 5 ProteinChip Array Forceps, 1 pair ProteinChip CHCA Energy Absorbing Molecules (EAM), 5 mg/vial, 20 ProteinChip SPA Energy Absorbing Molecules (EAM), 5 mg/vial, 20 Prot
