3.0 NGC™ Chromatography Systems and ChromLab™ Software Installation Guide Version 3.
NGC™ Chromatography Systems and ChromLab™ Software Installation Guide Version 3.
Bio-Rad Technical Support Department The Bio-Rad Technical Support department in the U.S. is open Monday through Friday, 5:00 AM to 5:00 PM, Pacific Time. Worldwide technical support is available on the Web at www.consult.bio-rad.com. Phone: 1-800-424-6723, option 2 Fax: 1-510-741-5802 Email: LSG.TechServ.US@Bio-Rad.com (U.S.) LSG.TechServ.Intl@Bio-Rad.com (International) Web: www.consult.bio-rad.
Table of Contents Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7 Main Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 Safety Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Safety Alerts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Safety Precautions . . . . . .
Table of Contents Attaching the Touch Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 NGC System Location Is on a Lab Bench. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 NGC System Location Is a Standard Cold Cabinet . . . . . . . . . . . . . . . . . . . . . 34 Connecting the Drain Tube to the Buffer Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 Installing the pH Probe in the pH Module . . . . . . . . . . . . . . . . . . . . . . . .
Table of Contents Plumbing NGC Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99 General Guidelines for Tubing Sizes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99 Plumbing All NGC Systems. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100 Plumbing the NGC Quest System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106 Plumbing the NGC Scout System . . . . . . . . . . . . . . . . . . .
Table of Contents Appendix C Connecting the C-96 Autosampler to NGC Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165 Using the C-96 Autosampler with NGC Systems . . . . . . . . . . . . . . . . . . . . . . . . 165 Connecting the C-96 Autosampler to the NGC Instrument . . . . . . . . . . . . . . 165 Plumbing and Priming the C-96 Autosampler. . . . . . . . . . . . . . . . . . . . . . . . . 168 Setting Up the C-96 Autosampler for Use in ChromLab Methods . . . . .
1 Introduction The NGC™ chromatography systems are preparative systems designed to rapidly automate the purification of biomolecules. The flexible, modular, and economical design makes NGC the instrument of choice for method development and scale-up. It is available in six preplumbed, factory-tested configurations at two different flow rates. Each preconfigured system can be further customized and upgraded by adding valves, detectors, or pumps in order to meet specific application needs.
1 | Introduction Main Features NGC chromatography systems enable you to do the following: 8 | Easily create purification and maintenance protocols from predefined method templates and protocol phases Automate multicolumn purification processes using preprogrammed templates and multiple column switching valves Automate multiple sample injections using either the sample inlet valve and the sample pump or the C-96 autosampler Expand sample monitoring using the signal import module (SIM) to
Safety Information Safety Information This section explains the different safety alerts within this document. This section also provides information about the safe use and setup of the instrument. Safety Alerts This document contains WARNINGS and Cautions pertaining to the installation and use of the NGC instrument. Alert Icon Definition Flammable/ Extreme Heat A hazardous situation that could result in serious personal injury or extreme damage to the instrument.
1 | Introduction Regulatory Information The NGC instrument has been tested and found to be in compliance with all applicable requirements of the following safety and electromagnetic compliance standards. The NGC instrument is labeled with the following compliance marks.
Regulatory Information FCC Warning and Notes The NGC instrument complies with part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) This device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. It is possible that emissions from the NGC instrument may interfere with some sensitive appliances when placed nearby or on the same circuit as those appliances.
1 | Introduction Finding Out More After you install NGC documentation from the NGC Chromatography Systems Documentation DVD, you can access installed NGC guides and tutorials on the Help menu in any ChromLab view. More information about the NGC chromatography systems and ChromLab software is available from the following sources. The NGC Chromatography Systems and ChromLab Software Instrument Guide is available on your NGC Chromatography Systems Documentation DVD as a .pdf file.
2 Preparing Your Site This chapter explains how to prepare your site to successfully install your NGC™ chromatography system and ChromLab™ software. Delivery Crate Dimensions The crated NGC instrument is very heavy, approximately 160–215 lb (72.6–97.6 kg) depending on the configuration. Bio-Rad strongly recommends that you use a pallet jack to move the instrument pallet from the shipping dock to the laboratory. Table 1.
2 | Preparing Your Site Table 1. Specifications for the NGC delivery crate, continued Item Specification NGC Scout Plus Length: 32 in.; 81.3 cm Width: 27 in.; 68.6 cm Height: 33 in.; 83.8 cm Weight: 172 lb; 78.1 kg NGC Discover™ Length: 32 in.; 81.3 cm Width: 27 in.; 68.6 cm Height: 41 in.; 104.1 cm Weight: 216 lb; 98.1 kg NGC Discover Pro Length: 27 in.; 68.6 cm Width: 27 in.; 68.6 cm Height: 14 in.; 35.6 cm Weight: 40 lb; 18.
Room Requirements Room Requirements Bench Space Ensure that the bench or rack on which you install the NGC system is designed to support its weight. Table 2. Specifications for the NGC instrument Item Specification NGC instrument dimensions Length: 24 in.; 61.0 cm Width: 19 in.; 48.3 cm Height (measured from lab bench): NGC Quest and NGC Scout series: 19 in.; 48.3 cm NGC Discover series: 26.5 in.; 67.3 cm NGC Discover Pro series: 34 in; 86.
2 | Preparing Your Site Table 2. Specifications for the NGC instrument, continued Item Specification Touch screen dimensions Height: 10 in.; 25.4 cm Length: Arm contracted: 13 in.; 33.0 cm Arm extended: 15 in.; 38.1 cm Width: Touch screen weight (with arm) Arm contracted: 1 in.; 2.5 cm Arm extended: 4 in.; 10.2 cm 4 lb; 1.8 kg Environment Table 3.
Power Requirements Power Requirements The power supply to the NGC system must be stable and within specifications at all times to ensure optimal operation. The power cable connected to the power inlet port must be rated for 7 amps or more. Table 4.
2 | Preparing Your Site ChromLab System Requirements Bio-Rad can provide a PC with ChromLab software preinstalled or you can install ChromLab onto a computer at your site. Table 5 lists the minium system requirements for the computer running ChromLab (known as the ChromLab computer). Table 5.
Preparing the ChromLab Computer Preparing the ChromLab Computer To ensure the ChromLab computer operates the NGC system smoothly and accurately displays ChromLab software you might need to Turn off sleep/hibernate mode Turn off automatic updates Set the display fonts To turn off sleep/hibernate mode 1. On the ChromLab computer, select Control Panel > Power Options and click Change plan settings for the selected power plan. 2.
2 | Preparing Your Site To turn off automatic updates 1. On the ChromLab computer, select Control Panel > Windows Update and choose Change settings in the left pane. 2. Under Important updates, choose Never check for updates (not recommended) from the dropdown list. 3. Click OK to save the setting and close the Change Settings window. To change the font size 20 1. On the ChromLab computer, select Control Panel > Fonts and choose Change font size in the left pane. 2.
3 Setting Up NGC Chromatography Systems After you unpack the NGC™ chromatography system and install it in a standard cold cabinet or on the lab bench you will need to Attach the touch screen Connect the drain tube to the buffer tray Connect the power and communication cables Connect the NGC system to the ChromLab™ computer This chapter explains how to perform these and other setup tasks.
3 | Setting Up NGC Chromatography Systems NGC Systems Accessories Kit Each NGC system ships with an accessories kit. The contents of the kit are specific to the system. Verify the contents against those listed in the sections that follow. The accessories kits contain the tubing, loops, and fittings necessary to complete the setup of the shipped configuration.
NGC Systems Accessories Kit NGC Scout Accessories Kit The kit for the NGC Scout™ system includes all the accessories in the NGC systems kit as well as Tube #2 (40 psi backpressure regulator to pH valve inlet) pH waste tubing pH calibration port inlet tubing Colored tubing for the buffer blending valve NGC Discover Accessories Kit The kit for the NGC Discover™ system includes all accessories in the NGC Scout kit as well as Pump head wash inlet tubing (2) for the sample pump Pump head
3 | Setting Up NGC Chromatography Systems Attaching the Touch Screen The installation procedure for the touch screen varies depending on the location of the NGC instrument (lab bench or standard cold cabinet). This section explains how to attach the touch screen to the instrument in either location. The communication cable for the touch screen is approximately 5' (1.52 m) in length. A 10' or 15' (3.0 or 4.57 m) cable is also available.
Attaching the Touch Screen Note: The top section in this image is a third tier. (The NGC Discover system is a 3-tier system.) The touch screen is attached at the top of the second tier. If you have an NGC Quest or an NGC Scout system, you will not have a third tier. Locate the side mount cover at the top of the second tier. 3. Using a Phillips screwdriver, remove the screw that secures the cable trough cover to the back of the instrument. Note: Store these components in a safe place for future use.
3 | 26 Setting Up NGC Chromatography Systems 4. Using two ¼-20 x ¾" hex bolts and the hex key labeled pumphead provided in the NGC accessories kit, attach the touch screen mount to the side of the NGC instrument. 5. Slide the touch screen display onto the mounting post. 6. Insert a ¼-20 x ¾" hex bolt underneath the mount to secure the touch screen.
Attaching the Touch Screen 7. Locate the touch screen display cable in the accessories kit. 8. Firmly push the touch screen cable into the port underneath the touch screen and tighten the thumbscrews. Tip: You can rotate the monitor if you need more room to insert the cable.
3 | Setting Up NGC Chromatography Systems 9. Route the display cable through the side and rear cable troughs on the instrument. 10. Insert the touch screen cable into the touch screen connector port on the back of the instrument and tighten the thumbscrews.
Attaching the Touch Screen 11. Locate the touch screen cable cover in the accessories kit. This is the cover with the cable notch. 12. Slide the touch screen cable cover into place to cover the cable.
3 | Setting Up NGC Chromatography Systems 13. Secure the cover to the back of the instrument with a 6-32 x 1 ¼" Phillips screw.
Attaching the Touch Screen Repositioning the Touch Screen Pivot Arm Assembly If you attach the touch screen to the right side of the NGC instrument, you must reposition its pivot arm assembly before mounting the touch screen onto the mounting bracket. To reposition the touch screen’s pivot arm assembly 1. Place the touch screen monitor face down on a clean surface to prevent scratching. 2.
3 | 32 Setting Up NGC Chromatography Systems 3. Using a Phillips screwdriver, remove the six screws that attach the touch screen to the pivot arm assembly and place them aside. 4. Lift the pivot arm assembly off of the touch screen. The mounting assembly should be in your right hand. 5. Turn the pivot arm assembly around 180° so that the mounting assembly is now in your left hand and insert the assembly into its position on the back of the touch screen. 6.
Attaching the Touch Screen 9. After you attach the mounting post to the side of the instrument, slide the touch screen display onto it. 10. Insert a ¼-20 x ¾" hex bolt underneath the mount to secure the touch screen.
3 | Setting Up NGC Chromatography Systems NGC System Location Is a Standard Cold Cabinet To connect the touch screen to the NGC system when the system is located in a cold cabinet perform the following tasks: Connect the touch screen and communication cables to the NGC instrument before inserting it into the cold cabinet Place the instrument in the cold cabinet Connect the touch screen to the touch screen stand Connect the touch screen cable to the touch screen These tasks are explained in
Attaching the Touch Screen 4. Complete the steps in the section To connect the power and communication cables on page 77. To place the instrument in the cold cabinet Caution: Two or more individuals should lift the instrument. Carefully lift the instrument into the cold cabinet. See the packaging insert for information about lifting the NGC instrument. To attach the touch screen to the touch screen stand 1. Place the touch screen monitor face down on a clean surface to prevent scratching. 2.
3 | 36 Setting Up NGC Chromatography Systems 3. Using a Phillips screwdriver, remove the six screws that attach the touch screen to the pivot arm assembly and place them aside. You will need them in step 6. 4. Locate the touch screen mounting bracket in the NGC Touch Screen Mounting Stand kit (#788-5040).
Attaching the Touch Screen 5. Position the bracket on the back of the touch screen so that the bracket opening is on the bottom. You should see the cable connector port through the bracket opening. 6. Attach the mounting bracket to the touch screen with the six screws that you set aside in step 3.
3 | Setting Up NGC Chromatography Systems 7. Locate the four thumb screws in the packaging for the touch screen stand. 8. Using the four thumb screws, loosely attach the touch screen bracket assembly to the stand. Then tighten the thumb screws evenly after you have completed the initial mounting. 9. Place the mounted touch screen on the table outside the cold cabinet. 10. Locate the end of the touch screen display cable that you routed through the access port. 11.
Connecting the Drain Tube to the Buffer Tray Connecting the Drain Tube to the Buffer Tray The top of the NGC system is molded to contain the buffer tray and route its drain tube to the right or left side of the instrument. The drain tube connects to the buffer tray through a connector on the back of the instrument top. Note: The drain tube connector is barbed to keep the tube tightly in position. After it is securely attached to the connector, the tube will be very difficult to remove.
3 | Setting Up NGC Chromatography Systems 4. Uncoil the drain tube and insert it through the drain hole on the side of the top. Caution: Ensure that no kinks are visible in the tube before connecting it to the connector. 5. Grasp the interior end of the tube and push it onto the drain connector as far back as possible to secure it in position. The tube should rest in the interior notch and be positioned so that it bends according to its natural coil.
Connecting the Drain Tube to the Buffer Tray 6. Turn the top right side up and place it on the NGC instrument. 7. Place the buffer tray on the top of the instrument and slide it all the way back until the black gasket is securely fixed to the front of the drain connector.
3 | Setting Up NGC Chromatography Systems Installing the pH Probe in the pH Module Note: This procedure assumes that the pH module is already installed in your system and the tubing is already connected to the appropriate positions. The pH probe is packaged in storage solution to protect it during shipping. To install the pH probe 1. Remove the cap from the pH valve flow cell. 2. Add a small amount of water (2 ml) to the pH valve flow cell. 3. Remove the pH probe from the storage solution. 4.
Installing the pH Probe in the pH Module 10. Start the NGC instrument. The following figure shows the pH probe installed in the flow cell. 11. Calibrate the pH monitor. Note: See the NGC Chromatography Systems and ChromLab Software User Guide for information about calibrating the pH monitor.
3 | Setting Up NGC Chromatography Systems Attaching an Expansion Tier to the NGC Instrument WARNING! Disconnect power to the NGC instrument before attaching an expansion tier. Do not attempt to service any component on the NGC instrument unless noted in this manual. Contact Bio-Rad for service requests. To attach the connector cable, you will need access to the back of the NGC instrument. If necessary, pull the NGC instrument away from the wall and rotate the instrument to gain access.
Attaching an Expansion Tier to the NGC Instrument Tip: The buffer tray can remain inside the top. 4. Locate the package that contains the expansion tier and 52" (1.32 m) connector cable. Important: The cables that ship with third and fourth tiers are not interchangeable. You must use the cable that ships with the expansion tier. Inserting a third tier cable into the fourth tier connector ports will damage the cable and the ports. 5. The catalog number for the 25" (63.
3 | Setting Up NGC Chromatography Systems 6. Place the expansion tier on top of the already installed tier. Tip: If your NGC instrument is on a lab bench, consider using a step stool to place the fourth tier onto the third tier. Tip: Although the images in this section show how to attach the fourth expansion tier to an already installed third tier, the procedure for attaching a third tier to a second tier is the same. These images are examples. Upper expansion tier Previously attached lower tier 7.
Attaching an Expansion Tier to the NGC Instrument 8. View the connector ends. Each end is beveled. The direction of the bevel determines the tier into which to insert the connector: The end with the bevel slanting up inserts into the upper tier’s connector port. The end with the bevel slanting down inserts into the lower tier’s connector port.
3 | Setting Up NGC Chromatography Systems 9. Firmly press the clips inward and insert the appropriate connector into the upper tier’s connector port. Tip: You hear a click when the connector is properly seated.
Attaching an Expansion Tier to the NGC Instrument 10. Insert the other connector into the lower tier’s connector port. 11. Place the top, with the buffer tray, on top of the NGC instrument and place the buffer bottles into the buffer tray. 12. Insert tubing lines into the sample and buffer bottles.
3 | Setting Up NGC Chromatography Systems 13.
Inserting Additional Modules Inserting Additional Modules WARNING! Disconnect power to the NGC instrument before inserting or removing any module. No user-serviceable parts are inside any component unless noted in this manual. Refer servicing questions to Bio-Rad service personnel. WARNING! To reduce the chance of liquid seeping into the instrument, all open bays must be filled with at least an empty module. The NGC instrument will not operate if it detects a slot without a module inserted.
3 | Setting Up NGC Chromatography Systems 5. (Optional) Convert the bay to a single-wide bay or a double-wide bay. See Converting Bays to Fit Modules on page 53. 6. Locate the module that you plan to install and remove it from its packaging. 7. The frame of the bay has tracks on the top and bottom of the right side. The module has guide rails on the top and bottom right side. Align the rails on the module with its track in the bay to properly guide it into position. Top of Module 8.
Inserting Additional Modules 10. Insert the screws that shipped with the module into the screw holes. Caution: Do not overtighten the screws. 11. Tighten the screws on the front of the module. Converting Bays to Fit Modules Some modules fit into single-wide bays while others require double-wide bays (such as the system and sample pump modules and the UV and UV/Vis detector modules). Bays can be converted from one size to the other by adding or removing the center divider.
3 | Setting Up NGC Chromatography Systems To convert a single-wide bay to a double-wide bay 54 1. Complete steps 1–4 in the section To insert or reposition modules on the NGC instrument on page 51. 2. Gently lift the divider upward off of the lip to unhook it and then pull it out of the instrument. 3. Store the divider in a safe place for later use. 4. Complete steps 6–11 in the section To insert or reposition modules on the NGC instrument.
Inserting Additional Modules To convert a double-wide bay to a single-wide bay 1. Complete steps 1–4 in the section To insert or reposition modules on the NGC instrument on page 51. 2. Locate a plastic center divider. 3. Insert the top of the divider into its slot. 4. Gently bend the divider and slide the bottom of the divider up onto the lip. 5. Complete steps 6–11 in the section To insert or reposition modules on the NGC instrument.
3 | Setting Up NGC Chromatography Systems Connecting Peripheral Equipment This section includes information about: Connecting the BioFrac Fraction Collector Connecting Column Clamps to the NGC Instrument Connecting a Keyboard or Mouse Connecting the Air Sensors Connecting the SIM to the NGC Instrument If you do not plan to connect these components to the NGC system, you can skip this section.
Connecting Peripheral Equipment 3. Insert system cable 17 that ships with the fraction collector into an INSTRUMENT BUS connector port. 4. Insert the other end of the cable into the INSTR. BUS connector on the back of the communication adaptor. Tip: The RX and DX diodes are communication indicators. You see the lights flash as data transfer between the fraction collector and the NGC instrument. The COMM port is not used to connect to the fraction collector. 5.
3 | 58 Setting Up NGC Chromatography Systems 6. Insert one end of the peripheral cable into the peripheral port on the communication adaptor. 7. Insert the other end of the cable into one of the peripheral ports on the NGC instrument.
Connecting Peripheral Equipment 8. Power on the instruments: a. Power on the BioFrac fraction collector first. b. Press the soft power switch on the NGC instrument to power it on. Connecting Column Clamps to the NGC Instrument The NGC instrument has indented metallic strips on both the right and left sides on which to mount column clamps, air sensors, and the fittings tightener. The column clamps can be placed at any position on the strips.
3 | Setting Up NGC Chromatography Systems Connecting Tubing to the Columns Note: Ensure that the system has been purged of air and that the tubing is filled with buffer before connecting the columns. To connect tubing to the columns 1. Connect tubing from one of the following modules to the top of the column: Sample inject valve column port Column switching valve inlet port (1–5) Note: For best air-free connection ensure that the tubing is dripping buffer at a flow rate of 0.5–1 ml/min.
Connecting Peripheral Equipment Connecting a Keyboard or Mouse The NGC systems can be controlled using the keyboard on the touch screen or by using a USB-connected keyboard and mouse. You can connect a keyboard and mouse to any of the four USB ports located on the right and left sides of the NGC instrument.
3 | Setting Up NGC Chromatography Systems Connecting the Air Sensors The system supports up to two air sensor modules and up to eight air sensors. The air sensor module has a magnetic backing and can attach to the side of the NGC instrument. Each module can contain up to four air sensors. The system detects the number of air sensors that are attached to the instrument. The air sensors can be set to detect air in lines connected to the system pumps to detect end of buffer.
Connecting Peripheral Equipment Air Sensor Tubing Length and Bubble Size The response of the air sensor module is highly dependent on the bubble size and flow rate, and requires a minimum tubing length between the sensor outlet and the pump inlet. Table 6 lists recommended tubing lengths for various flow rates and bubble sizes. Table 6.
3 | Setting Up NGC Chromatography Systems Attaching and Activating Air Sensors This section explains how to attach the air sensor module and the air sensors. It also explains how to activate the air sensors after they are attached. Note: Bio-Rad recommends that all buffers be filtered and degassed prior to use. To attach the air sensor module and air sensors 64 1. Ensure that the NGC instrument is powered off. 2.
Connecting Peripheral Equipment 5. 6. To connect an air sensor to a system pump: a. Connect tubing from the buffer bottle or from the outlet port on the one of the buffer inlet valves to one port on the air sensor. b. Connect tubing from the other port on the air sensor to the inlet port on the corresponding system pump. To connect air sensors to the buffer blending valve: a. Connect tubing from each buffer bottle to one port on each air sensor that will be used. b.
3 | Setting Up NGC Chromatography Systems Connecting External Devices to the NGC Instrument Tip: If you do not plan to connect the C-96 autosampler or other external devices to the NGC instrument you can skip this section. This section explains how to connect the signal import module (SIM) to the NGC instrument, and how to connect external devices to the SIM. For information about connecting the C-96 autosampler to the SIM, see Appendix C, Connecting the C-96 Autosampler to NGC Systems on page 165.
Connecting Peripheral Equipment Importing Analog Signals to the NGC Instrument You can import analog (voltage) signal from up to two external devices and convert it to digital NGC data via the SIM. The SIM imports and converts the signal to the appropriate trace units through its SIM 1 and SIM 2 ports.
3 | Setting Up NGC Chromatography Systems 3. 68 Insert the other end of the cable into one of the peripheral connector ports on the side of the NGC instrument.
Connecting Peripheral Equipment Connecting External Devices to the SIM WARNING! These procedures require you to work with exposed wires. Ensure that your systems are powered off and unplugged before performing this task. This section explains how to connect different external devices to the SIM. Before connecting the device, locate the connector ports that you will use on the back of the SIM.
3 | Setting Up NGC Chromatography Systems 3. Loosen all three screws on the top of the connector. Tip: These screws adjust the terminals that hold the wires in place. The screws do not dislodge from the connector. 4. Locate the analog output cable that ships with your detector. 5. If connectors are attached to both ends of the cable, remove the connector that does not insert into your detector. Tip: Alternatively, contact the manufacturer for a suitable cable. 70 6.
Connecting Peripheral Equipment 9. On the analog output cable, determine the positive and negative wires. 10. Insert the positive wire into the left terminal on the connector and tighten the screw. Note: Take care not to overtighten or fray the wire.
3 | Setting Up NGC Chromatography Systems 11. Insert the negative wire and one end of the jumper wire into the middle terminal on the connector and tighten the screw. 12. Insert the other end of the jumper wire into the right, or ground, terminal on the connector and tighten the screw.
Connecting Peripheral Equipment 13. Insert the connector into the SIM 1 or SIM 2 port on the back of the SIM. 14. Refer to the chapter System Controls in the NGC Chromatography Systems and ChromLab Software User Guide for information about how to enable signal import from the device.
3 | Setting Up NGC Chromatography Systems To connect an external device to the DAC A and DIGITAL OUT ports 1. On the back of the SIM, firmly grasp the DAC A connector and pull it straight out of its port. Note: ChromLab supports solely the DAC A port. When connecting an analog instrument, use the DAC port on the right (DAC A). 2. (Optional) If you want the NGC instrument to trigger the device to start measuring data, also remove the DIGITAL OUT connector and perform the steps that follow. 3.
Connecting Peripheral Equipment 9. Insert the positive and ground wires into the appropriate terminals on the DAC A connector and tighten the screws, taking care not to fray the wires. 10. Insert the trigger wire into terminal 2 on the DIGITAL OUT connector and tighten the screw, taking care not to fray the wire. Trigger wire Ground Positive wire wire 11. Insert the connectors into their ports on the back of the SIM. 12.
3 | Setting Up NGC Chromatography Systems Connecting the Power and Communication Cables The power sockets and communication ports for the NGC system are located on the back of the instrument. Because the back of the instrument will be very close to the back of the standard cold cabinet, it is recommended that you connect the power and communication cables before lifting the instrument into the cabinet.
Connecting the Power and Communication Cables To connect the power and communication cables 1. If you have not already done so, insert the end of the touch screen cable into the touch screen connection port on the back of the instrument. 2. Insert the Cat 6 (or higher) cable into the ethernet port on the back of the instrument. 3. (Optional) Connect the power cords for any peripheral instruments into the power outlet ports. 4. Connect the power cable to the power inlet port. 5.
3 | Setting Up NGC Chromatography Systems Connecting the NGC System to the ChromLab Computer Note: Ensure that the ChromLab computer and the NGC instrument are powered off before beginning this task.
Connecting the NGC System to the ChromLab Computer Establishing a Network Connection When establishing a connection between the NGC system and the ChromLab computer through your network infrastructure, ensure that they are both on the same subnet. A DHCP server is required so that the system receives an IP address. Note: The DHCP server should be configured to always assign the same IP address to the system. See your network administrator for more information.
3 | 80 Setting Up NGC Chromatography Systems | NGC Chromatography Systems and ChromLab Software
4 Installing ChromLab Software This chapter explains how to install ChromLab™ software on your computer. If this is the first time that ChromLab is installed on the computer, the installation wizard determines whether the required versions of .NET and SQL Server are installed. If the required versions are not installed, the wizard installs them before installing ChromLab. The wizard also installs and configures the ChromLab database instance.
4 | Installing ChromLab Software Installing ChromLab Software To install ChromLab software 1. Insert the ChromLab Software CD in your CD-ROM drive. The ChromLab installer automatically launches. 82 2. On the Welcome screen, click Next. 3. Accept the license agreement and click Next. 4. On the Ready to Install the Program screen, click Install.
Installing ChromLab Software The wizard installs ChromLab. Note: Installing ChromLab can take several minutes. 5. When the installation is complete, click Finish to exit the wizard.
4 | Installing ChromLab Software Establishing the Connection Between ChromLab Software and the NGC Systems Verify that you completed the following tasks: Connected the touch screen to the NGC instrument Connected all required peripheral equipment including: BioFrac™ fraction collector Air sensor module (if used) C-96 autosampler (if used) Connected the NGC instrument to the ChromLab computer Installed ChromLab on the ChromLab computer Powering on the NGC Instrument and Periphera
Establishing the Connection Between ChromLab Software and the NGC Systems 4. Press the soft power button on the side of the NGC instrument to power on the system.
4 | Installing ChromLab Software Setting the System Name The tasks in this section are performed on both the ChromLab computer and the NGC instrument’s touch screen. On the NGC system 1. When ChromLab Touch loads on the touch screen, you are prompted to provide a name for the NGC instrument. By default, the name is NGC. This is the name that appears on the ChromLab Home screen when it connects to the NGC system.
Establishing the Connection Between ChromLab Software and the NGC Systems 2. Touch or click OK. The ChromLab connection information dialog box appears. This dialog box explains how to connect the ChromLab computer to the NGC instrument. Follow the instructions provided on the connection dialog box. Note: Write down either the Network Name or the IP Address. You will need one of these values when you start ChromLab on the PC. Important: Do not click Continue at this time.
4 | Installing ChromLab Software On the ChromLab Computer 1. Start ChromLab. The System Connection dialog box appears. ChromLab detects the NGC systems available on the same sub-network or those systems that are directly connected to the computer. The system name, network name, and IP address of each available system appear in the table. Note: If your system does not appear in the table, click Detect to refresh the list. ChromLab searches the network for available NGC systems and refreshes the list. 2.
Establishing the Connection Between ChromLab Software and the NGC Systems 3. On the NGC instrument you see that the system is attempting to connect to the ChromLab computer. 4. When ChromLab successfully connects to the instrument, you see CONNECTED to at the bottom of the System Control pane.
4 | Installing ChromLab Software There are several possible causes for connection to fail. See Appendix B, Troubleshooting ChromLab Software Installation for more information and possible solutions. On the NGC Instrument On the touch screen click Continue. Note: You might see a dialog box indicating that the system time is not synchronized to the computer time.
Establishing the Connection Between ChromLab Software and the NGC Systems Click Restart System to synchronize the system time with the ChromLab computer time. Tip: This is a one-time action. After the system time and the ChromLab computer time are synchronized, the ChromLab computer manages the system time. When ChromLab successfully establishes a connection you see the fluidic scheme for the configured NGC system on the touch screen.
4 | 92 Installing ChromLab Software | NGC Chromatography Systems and ChromLab Software
Setting Delay Volume Setting Delay Volume The delay volume is the volume of fluid in the path between the UV or UV/Vis detector and the fraction collector drophead. The system is typically plumbed with a fixed length of tubing and a defined set of detectors (such as the conductivity monitor and pH detector) between the UV or UV/Vis detector and the fraction collector. The length of tubing and the volume of the flow cells between the UV or UV/Vis detector and the drophead define the delay volume.
4 | 94 Installing ChromLab Software 3. Select Synchronize with detector. 4. Refer to the following table to specify the inner diameter of the tubing in use. Tubing Color Inner Diameter Orange 0.02" (0.5 mm) Green 0.03" (.75 mm) Clear 0.062" (1.6 mm) 5. If your system includes the BioFrac fraction collector, select the checkbox Include BioFrac - 12 μl and specify the tubing length between the UV outlet port and the fraction collector drophead. 6.
Setting Delay Volume tubing length between the UV outlet port and outlet valve as well as the tubing length between the outlet valve and the fraction collector drophead. 8. Select or clear the next four checkboxes depending on whether or not these modules are present on your system. Note: If a pH detector module is present on your system and you select the checkbox to include its cell volume, determine whether the detector is set to Inline or Bypass mode and select the appropriate radio button. 9.
4 | Installing ChromLab Software Setting Up Remote Access Connection You can access the NGC instrument from your home, your desk, or anywhere else as long as you have access to the IP address of the NGC instrument. Remote access gives you full control of the NGC instrument via ChromLab Touch software as if you were physically present. Remote access requires the use of a VNC viewer, which employs VNC technology, an open standard. A variety of VNC viewers are available free on the web.
5 Plumbing and Priming the NGC Chromatography Systems With the exception of the inlet tubing, waste lines, and column, the NGC™ systems ship preassembled and preplumbed with the modules necessary to perform gradient separations. The systems require minimal postinstallation setup to prepare them to run simple gradient separations. This chapter explains how to plumb and prime your system after you have installed it and the ChromLab™ software.
5 | Plumbing and Priming the NGC Chromatography Systems Preparing the Buffer and Waste Bottles Before you plumb and prime the NGC instrument, prepare buffer and waste bottles. Buffer Bottles Place buffer bottles on the bench next to the instrument, or in the buffer tray on top of the instrument. The tray can accommodate up to six 1 L bottles. Waste Bottles Place the waste bottles on top of the bench or on the floor below the instrument.
Plumbing NGC Systems Plumbing NGC Systems Several plumbing tasks are required for all instrument configurations. Other tasks are specific to the configuration. This section explains how to plumb each instrument configuration. General Guidelines for Tubing Sizes The NGC instrument uses three sizes of tubing. Use the information in Table 7 to select the appropriate tubing. Table 7. NGC tubing sizes Use Tubing Dimensions Tubing Material Fittings Vol/cm Prepump 1/8" OD x 0.062" ID Clear 20 μl (3.
5 | Plumbing and Priming the NGC Chromatography Systems Plumbing All NGC Systems Table 8 lists the tasks for plumbing all NGC systems. The tasks are explained in detail in the sections that follow. Table 8. Plumbing tasks for all NGC systems Task 1. Inlet tubing to the pump head wash port on system pump A 2. Pump head wash priming line to the pump head wash port on system pump B 3. 1/4-28 union to the priming line on system pump B 4.
Plumbing NGC Systems System Pumps Wash Lines Sample Pump Wash Line Installation Guide | 101
5 | Plumbing and Priming the NGC Chromatography Systems To plumb and prime the system pump wash lines 1. Mount a pump head wash tube holder to the right or left side of the instrument in either the upper or lower rack slots. 2. Fill a 50 ml tube with 20% isopropanol (IPA) and insert it in the tube holder. Tip: You can use 20% ethanol as an alternative. 3. Connect inlet tubing to the pump wash inlet port (left rear port) on system pump A. 4.
Plumbing NGC Systems 8. Attach a 30 ml syringe to the 1/4-28 female to male luer adaptor. 9. Withdraw the plunger on the syringe until liquid is visible in the syringe. 10. Detach the luer adaptor and syringe from the pump head wash priming tubing on pump B. 11. Locate the second inlet tubing with the 1/4-28 union that you set aside in step 7. 12. Connect the union to the pump head wash priming tubing on system pump B. 13. Immerse the other end of the inlet tubing in the tube of 20% IPA.
5 | Plumbing and Priming the NGC Chromatography Systems Plumbing the Sample Inject Valve To plumb the sample inject valve 104 | 1. Connect waste tubing to waste ports Waste 1 and Waste 2 on the sample inject valve. 2. Connect an injection port to the center of the sample inject valve. 3. Connect the sample loop to ports Loop E and Loop F on the inject valve.
Plumbing NGC Systems Attaching the Backpressure Regulator If your column requires a high backpressure, install the backpressure regulator. To attach the backpressure regulator 1. Connect Tube #2 to the top of the conductivity monitor. 2. Connect the other end of Tube #2 to a 40 psi backpressure regulator. Note: The backpressure regulator has a raised arrow on its side. Ensure that the arrow on the regulator points in the direction of the flow.
5 | Plumbing and Priming the NGC Chromatography Systems Plumbing the NGC Quest System Table 9 lists the tasks for plumbing the NGC Quest™ system. The tasks are explained in detail in the sections that follow. Note: Ensure that you complete the plumbing tasks in the section Plumbing All NGC Systems on page 100 before continuing. Table 9. Plumbing tasks for the NGC Quest system Task 1. Inlet tubing to the system pump A inlet manifold 2. Inlet tubing to the system pump B inlet manifold 3.
Plumbing NGC Systems NGC Quest Point-to-Plumb Connections Installation Guide | 107
5 | Plumbing and Priming the NGC Chromatography Systems To plumb the system pumps 1. Connect inlet tubing to the inlet manifold of system pump A. 2. Connect inlet tubing to the inlet manifold of system pump B. 3. Immerse the open ends of both inlet tubing in a bottle of degassed, deionized water. To plumb the fraction collector 108 | 1. Connect Tube #4 to the open end of the backpressure regulator that you connected to the conductivity monitor in step 2 on page 105. 2.
Plumbing NGC Systems Plumbing the NGC Scout System Table 10 lists the tasks for plumbing the NGC Scout™ system. The tasks are explained in detail in the sections that follow. Note: Ensure that you complete the plumbing tasks in the section Plumbing All NGC Systems on page 100 before continuing. Table 10. Plumbing tasks for the NGC Scout system NGC Scout 1. Inlet tubing to ports Q1–Q4 on the buffer blending valve 2. Waste tubing to the calibration outlet port (Cal Out) on the pH valve 3.
5 | Plumbing and Priming the NGC Chromatography Systems NGC Scout Point-to-Plumb Connections 110 | NGC Chromatography Systems and ChromLab Software
Plumbing NGC Systems To plumb the buffer blending valve Connect the inlet tubing to ports Q1–Q4 on the buffer blending valve. Note: The buffer inlet tubing is color-coded to identify the different buffer solutions. The color scheme for the tubing and ports is defined as follows: Tubing color Port Solution Green Q4 High salt buffer Yellow Q3 Water Blue Q2 Buffer base Red Q1 Buffer acid To plumb the pH valve 1. Connect the pump head wash priming tubing to the Cal In port on the pH valve.
5 | Plumbing and Priming the NGC Chromatography Systems 3. Immerse the open end of the waste tubing in the waste container. 4. Connect Tube #2 from the 40 psi backpressure regulator that you connected in step 2 on page 105 to the inlet port (In) on the pH valve. 5. Connect Tube #4 to the outlet port (Out) on the pH valve. 6. Connect the other end of Tube #4 to the Common port on the BioFrac fraction collector diverter valve.
Plumbing NGC Systems Plumbing the NGC Discover System Table 11 lists the tasks for plumbing the NGC Discover™ system. The tasks are explained in detail in the sections that follow. Note: Ensure that you complete the plumbing tasks in the section Plumbing All NGC Systems on page 100 before continuing. Table 11. Plumbing tasks for the NGC Discover system Tasks 1. 2.
5 | Plumbing and Priming the NGC Chromatography Systems NGC Discover Point-to-Plumb Connections 114 | NGC Chromatography Systems and ChromLab Software
Plumbing NGC Systems To plumb the buffer blending valve Connect the inlet tubing to ports Q1–Q4 on the buffer blending valve. Note: The buffer inlet tubing is color-coded to identify the different buffer solutions.
5 | Plumbing and Priming the NGC Chromatography Systems To plumb the buffer inlet valves 1. Connect tubing from Outlet A on the buffer blending valve to port 8/BB on buffer inlet valve A. 2. Connect tubing from Outlet B on the buffer blending valve to port 8/BB on inlet valve B. 3. Connect tubing from the Out port on inlet valve A to the inlet manifold of system pump A. 4. Connect tubing from the Out port on inlet valve B to the inlet manifold of system pump B. 5.
Plumbing NGC Systems To plumb the pH valve 1. Connect the pump head wash priming tubing to the Cal In port on the pH valve. 2. Connect waste tubing to the Cal Out port on the pH valve. 3. Immerse the other end in the waste container. 4. Connect Tube #2 from the 40 psi backpressure regulator that you connected in step 2 on page 105 to the inlet port (In) on the pH valve. 5. Connect Tube #4 to the outlet port (Out) on the pH valve. 6.
5 | Plumbing and Priming the NGC Chromatography Systems Plumbing the NGC Discover Pro System Table 12 lists the tasks for plumbing the NGC Discover Pro system. The tasks are explained in detail in the sections that follow. Note: Ensure the you complete the plumbing tasks in the sections Plumbing All NGC Systems on page 100 and Plumbing the NGC Discover System on page 113 before continuing. Table 12. Plumbing tasks for the NGC Discovery Pro systems Tasks 1.
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5 | Plumbing and Priming the NGC Chromatography Systems Plumbing Sample Inlet Valves Each sample inlet valve module includes all necessary tubing. The inlet tubing is approximately 5' (1.5 m) in length. Determine the length of inlet tubing you need and cut the tubing to the appropriate length. Note: NGC inlet valves can be used for buffer or sample selection. To plumb the sample inlet valves 1. 120 | If your system has only one sample inlet valve: a.
Plumbing NGC Systems 2. 3. If your system has two sample inlet valves: a. Connect tubing from the Out port on sample inlet valve 1 (S1) to the sample pump inlet manifold. b. Connect tubing from the Out port on sample inlet valve 2 (S2) to port 8 on S1. c. Connect inlet tubing to ports 1–7 on S1 and to ports 1–8 on S2 as needed. Immerse the inlet tubing in sample containers. Plumbing Outlet Valves Each outlet valve module includes all necessary tubing. The tubing is approximately 5' (1.
5 | Plumbing and Priming the NGC Chromatography Systems b. c. 2. 3. 122 | (Optional) Do one of the following: Connect Tube #4 from port 1 to the Common inlet port on the BioFrac fraction collector diverter valve. Connect Waste tubing to port 1 and immerse in a waste container. Connect outlet tubing to the remaining ports as needed. If your system has two outlet valves: a. Connect tubing from the Out port on the conductivity monitor or pH monitor to the In port on outlet valve 1 (O1). b.
Priming and Purging the System Priming and Purging the System This section explains how to prime your system before running the pumps, and how to purge your system of air bubbles or rapidly exchanging buffer solutions. Note: This section assumes that you primed the wash system when you plumbed the system. See Plumbing All NGC Systems on page 100 for more information. Priming and purging tasks are specific to the NGC system. Table 13 lists the tasks associated with each system configuration.
5 | Plumbing and Priming the NGC Chromatography Systems Priming and Purging the NGC Quest System Caution: Before priming or purging the system, ensure that any attached columns are offline. The priming process occurs at a high flow rate and can significantly harm or destroy attached columns. Priming and purging the NGC Quest system requires access to ChromLab. You can perform the steps on the NGC touch screen. Important: The instrument’s buffer lines cannot be primed when the system is under pressure.
Priming and Purging the System 6. Remove the syringe from the priming port and discard the liquid into waste. 7. Repeat steps 2–6 for system pump B. To purge the system pumps 1. On the NGC touch screen, set the fluidic scheme to either NGC Quest or NGC Quest Plus. 2. In the fluidic scheme, touch or click the Sample Inject Valve module to open its dialog box. 3. Set the sample inject valve to System Pump Waste.
5 | Plumbing and Priming the NGC Chromatography Systems 126 | 4. In the fluidic scheme, touch or click the System Pump module to open its dialog box. 5. In the System Pump dialog box: a. Set Flow Rate to 20 ml/min. b. (Optional) Set Duration to at least 2 min. c. Clear the Enable Air Sensor checkbox. d. Click Start.
Priming and Purging the System Priming and Purging the NGC Scout System Priming the NGC Scout system is a manual procedure and involves priming the buffer blending valve starting from the Q4 port to Q1. Purging the NGC Scout system is an automated procedure and involves removing air from one or all buffer lines as well as the system pumps. Both procedures require access to ChromLab. Tip: You can perform the steps on the NGC touch screen.
5 | Plumbing and Priming the NGC Chromatography Systems 5. Select Q4 as the port to open and prime. 6. Insert a 30 ml syringe into the priming port on system pump A. 7. Turn the priming port valve counter-clockwise one full turn to open the seal. 8. Slowly withdraw the plunger to withdraw liquid from the port until air no longer enters the syringe (approximately 10 ml) and the tubing is filled with liquid. 9. Turn the priming port valve clockwise to close the port. 10.
Priming and Purging the System Purging the NGC Scout System To purge the buffer blending valve 1. 2. Open the System Pump dialog box and select Priming in the Mode dropdown list. To purge an individual line of air bubbles, proceed to step 2 on page 129. To purge all lines of air bubbles sequentially, proceed to step 3 on page 130. To manually purge an individual line of air bubbles and fill it with buffer: a. Select a port to open. b. (Optional) Change the default flow rate.
5 | Plumbing and Priming the NGC Chromatography Systems 3. To purge all lines of air bubbles and fill them with buffer: a. Click All. b. (Optional) Change the default flow rate. The default flow rate is set to 20 ml/min. c. Click Start. The system pumps and the buffer blending valve start operating at the specified flow rate, exchanging the solution in the inlet lines and expelling any trapped bubbles, cycling sequentially through the ports Q1 to Q4.
Priming and Purging the System Priming and Purging the NGC Discover System Priming the NGC Discover system is a manual procedure and involves priming the buffer blending valve starting from the Q4 port to Q1 then the buffer inlet valve starting from buffer inlet A1. Purging the NGC Discover system is an automated procedure and involves removing air from one or all buffer lines as well as the buffer inlet valves and the system and sample pumps. Both procedures require access to ChromLab.
5 | Plumbing and Priming the NGC Chromatography Systems 4. In the fluidic scheme, touch or click the System Pump module to open its dialog box. 5. In the System Pump dialog box, select Priming on the Mode dropdown list. Note: When in Priming mode, the sample inject valve switches the system pump flow to waste (W2). After the process is completed, the sample inject valve switches the system pump to manually load the column through the loop. 6. 132 | Select Q4 as the port to open and prime.
Priming and Purging the System 7. Insert a 30 ml syringe into the priming port on system pump A. 8. Turn the priming port valve counter-clockwise one full turn to open the seal. 9. Slowly withdraw the plunger to withdraw liquid from the port until air no longer enters the syringe (approximately 10 ml) and all tubing is filled with liquid. 10. Turn the priming port valve clockwise to close the port. 11. Remove the syringe from the priming port and discard the liquid into waste. 12.
5 | Plumbing and Priming the NGC Chromatography Systems To prime the buffer inlet valves 134 | 1. Immerse the tubing for the inlet ports in their respective buffer containers. 2. On the NGC touch screen, open the System Pump dialog box. 3. In the System Pump dialog box, select the first inlet port to prime, for example Buffer A 1. 4. Insert a 30 ml syringe into the priming port on system pump A. 5. Turn the priming port valve counter-clockwise one full turn to open the seal. 6.
Priming and Purging the System 9. Repeat steps 4–8 for the remaining buffer inlet ports. 10. To prime buffer inlet valve B, select buffer B1 and perform steps 3–9 on system pump B. To prime the sample pump 1. Immerse the inlet tubing for the sample pump in the appropriate container. 2. Insert a 30 ml syringe into the priming port. 3. Turn the priming port valve counter-clockwise one full turn to open the seal. 4.
5 | Plumbing and Priming the NGC Chromatography Systems To prime the sample inlet valve (if available) 136 | 1. Immerse the tubing for the inlet valve in the appropriate buffer container. 2. On the ChromLab computer or the touch screen, start ChromLab in Manual mode. 3. In the fluidic scheme, touch or click the Sample Pump module with the sample inlet valve to open its dialog box. 4. In the Sample Pump dialog box, select the first sample inlet port to prime.
Priming and Purging the System 5. Insert a 30 ml syringe into the sample pump’s priming port. 6. Slowly withdraw the plunger to withdraw liquid from the port until air no longer enters the syringe (approximately 10 ml). 7. Remove the syringe from the priming port and discard the liquid into waste. 8. Repeat steps 4–7 for the remaining sample inlet ports.
5 | Plumbing and Priming the NGC Chromatography Systems Purging the NGC Discover System To purge the buffer blending valve 1. Open the System Pump dialog box and select Priming on the Mode dropdown list. Note: When in Priming mode, the sample inject valve switches the system pump flow to waste (W2). After the process is completed, the sample inject valve switches the system pump to manually load the column through the loop.
Priming and Purging the System 2. To purge an individual line of air bubbles and fill it with buffer: a. Select the port to open. b. (Optional) Change the default flow rate. The default flow rate is 20 ml/min. 3. c. Click Start. d. Repeat steps 2a–c for any other port to purge. To purge all lines of air bubbles and fill them with buffer: a. Select All. b. (Optional) Change the default flow rate. The default flow rate is set to 20 ml/min. c. Click Start.
5 | Plumbing and Priming the NGC Chromatography Systems To purge the buffer inlet valves 1. Open the System Pump dialog box and select Priming on the Mode dropdown list. Note: When in Priming mode, the sample inject valve switches the system pump flow to waste (W2). After the process is completed, the sample inject valve switches the system pump to manually load the column through the loop. 2. Select the first buffer port to purge, for example Buffer A 1. 3. (Optional) Change the default flow rate.
Priming and Purging the System To purge the sample pump 1. In the fluidic scheme, touch or click the Sample Pump module to open its dialog box. 2. In the Sample Pump dialog box, set Flow Rate to 10 ml/min.
5 | Plumbing and Priming the NGC Chromatography Systems 3. Click Change Position to set the sample inject valve to Sample Pump Waste and close its dialog box. 4. Click Start. To purge the sample inlet valve (if available) 142 | 1. In the fluidic scheme, touch or click the Sample Pump module with the sample inlet valve to open its dialog box. 2. In the Sample Pump dialog box, do one of the following: If only one sample inlet valve is present, select S1 Port 8 as the port to purge.
Priming and Purging the System 3. Set Flow Rate to 10 ml/min. 4. Click Change Position to set the sample inject valve to Sample Pump Waste and close the dialog box. 5. (Optional) When an air sensor is present, in the Sample Pump dialog box, select Enable Air Sensor to use end-of-sample detection to stop the pump. 6. Click Start. 7. Monitor the pump pressure and outlet flow to ensure that no air is in the lines and that the fluid is flowing properly.
5 | Plumbing and Priming the NGC Chromatography Systems Calibrating the Instrument The NGC instrument includes integrated pressure and temperature sensors. The pressure sensors monitor the system pressure to protect the column and media from overpressure. The temperature sensor in the conductivity monitor enables the system to provide real-time temperature compensation for conductivity and pH measurements. The measured temperature appears as a trace on the chromatogram.
Calibrating the Instrument Calibrating the Pump Flow Rate Prime and purge the system pumps with water before starting the calibration procedure. Calibrate the flow rate with water. Note: Ensure that the column is offline before performing this task. To calibrate the pump flow rate 1. On the NGC touch screen, select Calibrate on the ChromLab dropdown menu. 2. Select Pump Flow Rate on the dropdown list. 3. Follow the steps in the Pump Flow Rate Calibration dialog box. 4.
5 | Plumbing and Priming the NGC Chromatography Systems Calibrating the Conductivity Monitor Flow conductivity standard from any commercial supplier through the conductivity flow cell until a stable reading is obtained. To calibrate the conductivity monitor 146 | 1. On the NGC touch screen, select Calibrate on the ChromLab dropdown menu. 2. Select Conductivity Monitor on the dropdown list. 3. Follow the steps in the Conductivity Monitor dialog box. 4.
A Upgrading ChromLab Software and NGC Systems This appendix explains how to upgrade ChromLab™ software and your NGC™ system from a previous version to the current version. Caution: Before beginning the upgrade process, ensure that you have saved all manual runs, and that no methods are running. Upgrading ChromLab Software To upgrade ChromLab software to the current version 1. Insert the ChromLab Software CD into your CD-ROM drive. The ChromLab installer automatically launches.
A | Upgrading ChromLab Software and NGC Systems Updating NGC Systems Updating the NGC system is a two-step process: Updating ChromLab software on the instrument Updating the firmware on the instrument Between the software and firmware update the NGC instrument automatically reboots. The instrument reboots following a successful firmware upgrade as well. To update the NGC system 1. On the ChromLab computer, start ChromLab.
Updating NGC Systems 3. After the update process completes, the instrument automatically reboots. The system checks the firmware versions on the modules. If the firmware is not current the Firmware Version Warning dialog box appears. Click Update Now. A progress dialog box appears on the NGC touch screen with information about the update including the module that is being updated and the time remaining for that update.
A | 150 | Upgrading ChromLab Software and NGC Systems NGC Chromatography Systems and ChromLab Software
B Troubleshooting ChromLab Software Installation This appendix explains what to do if ChromLab™ software fails to connect to the NGC™ system. Possible Causes for Connection Failures When ChromLab fails to connect to an NGC system, the following error message appears: Table 14 on page 152 lists several possible causes for the connection to fail.
B | Troubleshooting ChromLab Software Installation Table 14. Possible Causes and Solutions for Connection Failures Possible Cause Solution The network name or IP address entered is incorrect. 1. Verify that the NGC instrument is available on the network. See Troubleshooting Network Connection Issues on page 158 for information about ensuring the NGC system is available on the network. 152 | 2. On the error message in ChromLab, click Retry. 3.
Possible Causes for Connection Failures Table 14. Possible Causes and Solutions for Connection Failures, continued Possible Cause Solution A firewall on the ChromLab computer is turned on and does not allow the ChromLab programs and services to run. 1. Check your firewall configuration and ensure that the required ChromLab programs and services are allowed to run. See ChromLab Software Firewall Settings on page 162 for the list of required ChromLab programs and services.
B | Troubleshooting ChromLab Software Installation Configuring the Network Adapter for Direct Connection When connecting the ChromLab computer to the NGC system through a direct cable connection, the network adapter on the ChromLab computer must be configured to use TCP/IP 4.0 with DHCP. This section explains how to configure Local Area Connection on the ChromLab computer. To configure Local Area Connection 154 | 1.
Configuring the Network Adapter for Direct Connection 3. Review the list of network connections. If you see at least one icon labeled Local Area Connection proceed to step 4. Note: If you do not see this icon, Microsoft Windows does not recognize the network adapter. This could be a driver problem with Windows. Contact the vendor of the computer or your local IT department to solve this problem. 4.
B | Troubleshooting ChromLab Software Installation 156 | 5. Right-click the Local Area Connection icon and select Properties. 6. In the Local Area Connection Properties dialog box, locate the Internal Protocol version 4 (TCP/IPv4) list item.
Configuring the Network Adapter for Direct Connection Note: If you do not see this item listed, the computer is not set up for TCP/IP. Refer to the vendor for information about enabling TCP/IP. 7. Select Internet Protocol Version 4 (TCP/IPv4) and click Properties. The Internet Protocol version 4 (TCP/IPv4) Properties dialog box appears. 8. Make sure that Obtain an IP address automatically and Obtain DNS server address automatically are selected and click OK. 9.
B | Troubleshooting ChromLab Software Installation Troubleshooting Network Connection Issues This section explains what to do if ChromLab is unable to connect to the NGC instrument because the IP address of the instrument has changed. Locating the IP Address of the NGC Instrument The IP address of the NGC instrument is available on the initial connection screen as well as in the System Information dialog box.
Troubleshooting Network Connection Issues IP Address Located in the System Information Dialog Box If the system is running, you can find the IP address of the NGC instrument in the General tab in System Information. To locate the IP address in the System Information dialog box 1. On the touch screen, expand the system’s drop-down list and select System Information. 2. Locate System IP Address in the General tab. 3. Write down the IP address.
B | Troubleshooting ChromLab Software Installation Checking the Network Connection After you find the IP address of the instrument, from the ChromLab computer verify that the instrument can be accessed through the network. To check the network connection 1. On the ChromLab computer, open a Command Prompt window. 2. At the prompt, type > ping x.x.x.x where x.x.x.x is the IP address of the instrument.
Troubleshooting Network Connection Issues If the network is not set up correctly, you see results similar to this image: The ChromLab computer cannot connect to the instrument. Check the setup of the network adapter in the section Configuring the Network Adapter for Direct Connection on page 154. Make sure TCP/IPv4 is installed and set up for DHCP.
B | Troubleshooting ChromLab Software Installation ChromLab Software Firewall Settings If your computer has Windows Firewall turned on, check your firewall settings and ensure that the following programs and services required for ChromLab are allowed to communicate with your computer. Important: If you have anti-virus software installed on your ChromLab computer, configure the software to allow the following programs and services to communicate with ChromLab.
Firmware Update Failure Firmware Update Failure If the firmware upgrade fails, restart the NGC instrument. The software attempts the firmware update again. If the firmware update fails again: 1. Shut down the NGC instrument. 2. Turn off the power at the back of the instrument for 30 seconds. 3. Power on the NGC instrument. The firmware update procedure starts again.
B | Troubleshooting ChromLab Software Installation 164 | NGC Chromatography Systems and ChromLab Software
C Connecting the C-96 Autosampler to NGC Systems Using the C-96 Autosampler with NGC Systems The C-96 autosampler enhances NGC™ chromatography systems, providing automated, accurate, and reproducible sample injections. Its easy-to-install accessories allow for a wide range of injection volumes from 5 μl–5 ml. The signal import module (SIM) connects the C-96 autosampler to NGC systems. The SIM digitizes the analog signal from the C-96 autosampler and transmits the signal to the NGC system.
C | Connecting the C-96 Autosampler to NGC Systems 5. Locate the two cables that connect the autosampler to the SIM: 15-pin D connector cable (part #10027227) 9-pin D connector cable (part #10027224) These cables ship with the autosampler. 6. Attach the DIGITAL IN connector to the 15-pin D connector cable. Tip: The 15-pin D connector cable has three wires (red, black, and brown). a. From left to right, loosen the first, second, and last screws on the connector. b.
Using the C-96 Autosampler with NGC Systems 8. 9. On the back of the autosampler: a. Insert the 15-pin cable into the left (P1 RELAYS OUTPUTS) connector port and tighten the thumbscrews. b. Insert the 9-pin cable into the right (P2 INPUTS) connector port and tighten the thumbscrews. On the back of the SIM: a. Insert the P1 RELAYS OUTPUTS cable into the DIGITAL IN port. b. Insert the P2 INPUTS cable into the DIGITAL OUT port.
C | Connecting the C-96 Autosampler to NGC Systems Plumbing and Priming the C-96 Autosampler This section explains how to plumb the C-96 autosampler to the sample inject valve on the NGC instrument and to prime the autosampler after you plumb it. Before you plumb and prime, you must prepare the tubing. Use 1/16" PEEK tubing to plumb the autosampler to the sample inject valve.
Using the C-96 Autosampler with NGC Systems To prime and purge the C-96 autosampler Note: Before priming the autosampler, ensure that any column is offline. 1. Immerse the inlet tubing in a container of buffer or HPLC grade (filtered, degassed) or other high quality water. 2. Use a syringe to prime the pumps. See the section Priming and Purging the System on page 123 for specific information. 3. On the ChromLab™ computer or the touch screen, start ChromLab in Manual mode. 4.
C | 170 | Connecting the C-96 Autosampler to NGC Systems 6. Set the inject valve to System Pump Inject Loop. 7. In the fluidic scheme, touch or click the System Pump module to open its dialog box. 8. In the System Pump dialog box: a. Set Flow Rate to 5 ml/min. b. (Optional) Set Duration to at least 2 min. c. Click Start.
Using the C-96 Autosampler with NGC Systems Installation Guide | 171
C | Connecting the C-96 Autosampler to NGC Systems Setting Up the C-96 Autosampler for Use in ChromLab Methods This section briefly explains how to set up the autosampler for use in a ChromLab method. For information about creating methods in ChromLab, see the NGC Chromatography Systems and ChromLab Software User Guide. To set up a method that uses the autosampler 1. In ChromLab create a method that includes the autosampler in the fluidic scheme.
D Firewall Configuration Firewall Inbound Rules for Bio-Rad NGC Software Bio-Rad configures the ChromLab™ software using Microsoft Windows firewall with advanced security. If you have a third-party firewall application you must configure that software so that it meets Bio-Rad software’s firewall rules. The rules for NGC™ are listed in Table 15 on page 174. You can see the complete list of inbound firewall rules in the Windows Firewall with Advanced Security tool.
D | Firewall Configuration For all services and applications listed in Table 15, the following rules apply: Enabled Action Allowed Users Allowed Computers Yes Allow Any Any Table 15.
Bio-Rad Laboratories, Inc. Web site www.bio-rad.