Instruction Manual

2 Technical Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
1.2 Gel Formulations
Criterion precast gels are available in a range of formulations for virtually every electrophoresis
application (Table 1.1). All Criterion gels are composed of polyacrylamide with a bisacrylamide
crosslinker, and they are available in a selection of single percentages and gradients.
Table 1.1. Criterion precast gel formulations.
Application Gel Formulation Sample Buffer Running Buffer
SDS-PAGE Criterion Tris-HCl Laemmli Tris/glycine/SDS
Criterion Stain Free
Criterion TGX
™
Criterion TGX Stain-Free
Criterion
™
XT Bis-Tris XT XT MOPS or XT MES
Criterion XT Tris-acetate XT XT Tricine
Native PAGE Criterion Tris-HCl Native Tris/glycine
Criterion Stain Free
Criterion TGX
Criterion TGX Stain-Free
Criterion XT Tris-acetate
Peptide analysis Criterion Tris-Tricine Tricine Tris/Tricine/SDS
Isoelectric focusing (IEF) Criterion IEF IEF Anode and cathode buffers
Protease detection Criterion zymogram Zymogram Tris/glycine/SDS
dsDNA separation Criterion TBE Nucleic acid Tris/boric acid/EDTA (TBE)
ssDNA and RNA separation Criterion TBE-urea TBE-urea TBE
1.3 Comb Configurations
Comb Type Well Volume
12+2 well
1
45 μl with two 15 μl reference wells
18-well 30 μl
26-well
1
15 μl
Prep+2 well 800 μl with two 15 μl reference wells
IPG+1 well 11 cm ReadyStrip
™
IPG strip (450 μl) with one 15 μl reference well
1.4 Specifications
Gel material Polyacrylamide
Gel dimensions (W x L) 13.3 x 8.7 cm
Gel thickness 1.0 mm
Resolving gel height 6.5 cm
Cassette dimensions (W x L) 15.0 x 10.6 cm
Cassette material Styrene copolymer
Comb material Polycarbonate
Running buffer 460 ml (per gel)
1
Multichannel pipet compatible.
Criterion Precast Gels