Page 1 PUMP 8:09 AM GRADIENT 8/14/2003 ECONO 4006161B.
4006161B.qxd 8/14/2003 8:09 AM Page i Safety SAFETY ! Caution/Warning Disconnect power to the Econo Gradient Pump before servicing. No user-serviceable parts are inside the unit. Refer servicing to Bio-Rad service personnel. The Bio-Rad Econo Gradient Pump is certified to meet the I.E.C. 1010-1* standard for safety of laboratory equipment. Certified products are safe to use when operated in accordance with the instruction manual.
4006161B.qxd 8/14/2003 8:09 AM Page iii Table of Contents TABLE OF CONTENTS Safety .............................. i Section 1 Introduction . . . . . . . . . . . . . . . . . . . . 1 Section 2 2.1 2.2 Unpacking and Setting Up . . . . . . . . . Unpacking Instructions . . . . . . . . . . . . . Voltage Conversion . . . . . . . . . . . . . . . 2 2 3 Section 3 3.1 3.2 Physical Description and Control Features 4 Front Panel Functions . . . . . . . . . . . . . 4 Rear Panel Functions . . . . . . . . .
006161B.qxd 8/14/2003 8:09 AM Page iv Table of Contents Appendix C Warranty . . . . . . . . . . . . . . . . . . . . . . . 43 Appendix D Ordering Information . . . . . . . . . . . . .
4006161B.qxd 8/14/2003 8:09 AM Page 1 Introduction 1.0 INTRODUCTION The Econo Gradient Pump (EGP) is a two-channel, bi-directional, variable speed peristaltic pump for low-pressure chromatography and general laboratory use. It offers a full range of features to facilitate ease of use as a stand-alone pump or as a part of the BioLogic Duo-Flow System running software version 3.0 or later.. As a stand-alone pump, the EGP delivers flow rates from 2 µl/min to 40 ml/min using a single channel of 0.
4006161B.qxd 8/14/2003 8:09 AM Page 2 Unpacking 2.0 UNPACKING AND SETTING UP 2.1 UNPACKING INSTRUCTIONS Carefully remove the unit from the shipping box, lifting from the handle on the back of the pump or from the bottom of the instrument. Remove the plastic bag and inspect the instrument for any external damage. Figure 2-1 shows the contents of the box. Model EGP Econo Pump Low-Pressure Fittings Kit Power Cord System Tubing Pumphead Tubing Figure 2-1. Parts supplied with the Econo Gradient Pump.
4006161B.qxd 8/14/2003 8:09 AM Page 3 Unpacking Your Econo Gradient Pump was carefully tested at the factory and was shipped in good working order. If any part is missing or damaged, contact your local Bio-Rad office immediately. 2.2 VOLTAGE CONVERSION Warning: The Econo Gradient Pump is shipped in its 100/120 V configuration. To operate at 220/240 V, refer to the procedure in section 7, Cleaning and Maintenance, to make the conversion.
4006161B.qxd 8/14/2003 8:09 AM Page 4 Description 3.0 PHYSICAL DESCRIPTION AND CONTROL FEATURES 3.1 FRONT PANEL FUNCTIONS Platen ECONO GRADIENT PUMP Reverse Purge LCD Display Run Next Cancel Soft Keys Arrow Keys Remote Setup Manual Mode Step Program Figure 3-1. Front Panel Controls and Displays.
4006161B.qxd 8/14/2003 8:09 AM Page 5 Description Front Panel Function [LCD; see figure 3-1] LCD Display This display has two rows of 16 characters. Different windows will appear on this display depending on the requests made by the user. Some windows will display operating parameters, while others will allow entry of variables such as flow rates, valve position and programming steps. Choices are shown in the lower row, above one of the three soft keys. A selection is indicated in uppercase letters.
4006161B.qxd 8/14/2003 8:09 AM Page 6 Description Front Panel Function Cancel Key Cancel Pressing Cancel will clear an entry that has been input with either the arrow or soft keys. If no entry has been made, the previous window will be displayed. This allows passage through the windows in the reverse direction. Run/Stop Key Run This key is used to start or stop the pump in both Manual mode and Program mode operation. The pump will run in the clockwise direction by factory default.
4006161B.qxd 8/14/2003 8:09 AM Page 7 Description Front Panel Function Purge Key Purge This key is used to prime and purge tubing lines without disturbing the primary speed setting of the pump. When Purge is pressed, the pump will run at maximum speed (25 rpm), leaving the connected Gradient Mixer and Aux valve operating and the fraction collector and chart recorder off. The yellow purge LED is illuminated when purging. This key is not operable when the pump is running in Program mode.
4006161B.qxd 8/14/2003 8:09 AM Page 8 Description 3.2 REAR PANEL FUNCTIONS Made in U.S.A. Model No. Econo Gradient Pump Serial No. 491 BR AC Voltage Max Current 0.4 A 0.2 A Frequency 50-60 Hz 50-60 Hz Fuse Type 0.4 A 0.2 A CAUTION WARNING Warning: Disconnect supply before servicing. Advertissement:: Couper l'alimentation avant l'entretien et depannage.
4006161B.qxd 8/14/2003 8:09 AM Page 9 Description Display Function Aux Valve Connector This 8-pin connector controls auxiliary valves such as the optional SV-3 Diverter valve or the optional Splitter valve. I/O Connector This 8-pin outlet provides start signals to the Model 1327 Chart Recorder, the Model 2110 Fraction Collector, or the Model 2128 Fraction Collector.
4006161B.qxd 8/14/2003 8:09 AM Page 10 Tubing Selection 4.0 TUBING SELECTION AND INSTALLATION 4.1 TUBING SELECTION The Econo Gradient Pump may be used with most flexible tubing having an inner diameter less than or equal to 3.2 mm (1/8”), and a wall thickness of 1.0 mm or less, including Silicone, Tygon, and PharMed. Silicone tubing, the most inert of the three, is suitable for aqueous and polar solutions. Tygon is suitable for most aqueous solutions.
4006161B.qxd 8/14/2003 8:09 AM Page 11 Tubing Selection When using tubing other than precut tubing from Bio-Rad, the tubing must be cut to the following lengths to accommodate prestretch: Tubing Tygon, PharMed Silicone Length 179 mm +/- 1.5 (7.04” +/- 0.05) 171 mm +/- 1.5 (6.75” +/- 0.05) Install tubing onto the pump head as described in Section 4.2. Warning: If using tubing other than the type supplied by BioRad, make sure the wall thickness is not greater than 1.0 mm.
4006161B.qxd 8/14/2003 8:09 AM Page 12 Tubing Selection 4.2 TUBING INSTALLATION 1. Referring to Figure 4-2, pull the platen cam lever away from the pump head to unlock the platen and slide the platen away from the pump head frame assembly, exposing the rollers. 2. Slip a lock-ring onto one end of the tubing. See below for the size and color of each lock-ring. Insert a barbed female luer fitting into the same end until the tubing reaches the flange of the fitting.
4006161B.qxd 8/14/2003 8:09 AM Page 13 Tubing Selection 3. Insert one end of the tubing into the tubing retaining bracket of the pump head. Lightly pull the tubing around the rollers to remove slack. Attach the other end of tubing into the tubing retaining bracket on the opposite side of the pump head. 4. Slide the platen back into the pump head frame assembly until it rests up against the tubing. Press the cam lever in toward the pump head, locking the platen up against the tubing and rollers.
4006161B.qxd 8/14/2003 8:09 AM Page 14 System Operation 5.0 SYSTEM OPERATION There are four modes of system operation: • Remote • Setup • Manual • Program, including running the system 5.1 REMOTE MODE The Econo Gradient Pump can be connected to the BioLogic Duo-Flow Workstation running software version 3.0 or later with an Instrument bus cable (System cables 17, 18, 19, or 21).
4006161B.qxd 8/14/2003 8:09 AM Page 15 System Operation Screen 1. Flow Rate Selection. Use the soft keys to select either RPM (revolutions per minute) or ml/m. This information will be used in writing a protocol from the Program mode and in using the Manual mode. • If RPM is selected, the user may control the pump speed in revolutions per minute (RPM). Pressing Next skips to screen 9. • If ML/M is selected, the user is required to calibrate the pump.
4006161B.qxd 8/14/2003 8:09 AM Page 16 System Operation Screen 4. Pump Calibration by Measured Flow. Pressing the left or right soft keys will start the pump for the selected time. Collect the pump effluent in a graduated cylinder. During calibration, the pump runs at half speed (12.5 RPM). Typical volumes collected after a 2-minute run are 20 ml, 6.4 ml, 1.7 ml, and 700 µl for tubing IDs of 3.2 mm, 1.6 mm, 0.8 mm, and 0.51 mm respectively. The volume collected is entered in screen 5.
4006161B.qxd 8/14/2003 8:09 AM Page 17 System Operation Screen 6. Selecting Units for Programming. Programming steps can be described in either units of time (in minutes) or volume (in ml). 6 Program Steps TIME vol Pressing the left soft key selects time; the right key selects volume. Pressing Next advances to screen 8 if a Splitter valve is present or screen 9 if a Splitter valve is not present. Screen 7 (not displayed). This is available for future upgrades. Screen 8. Splitter Valve Period Selection.
4006161B.qxd 8/14/2003 8:09 AM Page 18 System Operation Screen 9. Biologic System Address Number. The Econo Gradient Pump can be connected to the BioLogic HR or BioLogic Duo-Flow workstation via an Instrument bus cable (System cables 17, 18, 19, or 21). When connected, the address of the Econo Gradient Pump must correspond to the address recognized by the BioLogic System. This address is only relevant when the Econo Gradient Pump is connected to the BioLogic System. The default address is 1.
4006161B.qxd 8/14/2003 8:09 AM Page 19 System Operation Screen 12. Beeper. The beeper can be switched on or off at the user’s discretion. The beeper, which beeps on each key press, can be disabled. 12 ON Beeper off Using the soft keys, select the desired option and press Next to accept and advance to screen 13. Screen 13. Erase Program Option. The Econo Gradient Pump will store one program in its non-volatile memory. The program stored in the Program mode can be erased by selecting the “yes” option.
4006161B.qxd 8/14/2003 8:09 AM Page 20 System Operation 5.3 MANUAL MODE The Econo Gradient Pump and attached devices can be controlled using the pump’s front panel membrane keys and LCD display while in Manual mode. Press the Mode key until the Manual LED is illuminated. Up to three parameters (depending on how many optional devices are connected to the Econo Gradient Pump) can be controlled. Choose a field on the LCD by pressing the corresponding soft key.
4006161B.qxd 8/14/2003 8:09 AM Page 21 System Operation 5.3.3 Setting Auxiliary (SV-3 or Splitter) Valve Control Control for an auxiliary valve is selected by pressing the right soft key. This option is available if a valve is connected to the Auxiliary port on back of the Econo Gradient Pump. The valve type is automatically recognized. The SV-3 diverter valve must be connected if you wish to program a collection window with either the model 2110 or 2128 Fraction collector.
4006161B.qxd 8/14/2003 8:09 AM Page 22 System Operation Each field may be selected with the corresponding soft key. The arrow keys are then used to increment or decrement the value. Pressing the Step key or the Next key will accept the displayed values and advance to the next step. The program end occurs when either 6 steps have been entered, or two identical steps (pressing Step twice) are entered sequentially. If an auxiliary valve is present, a screen will appear after programming is completed.
4006161B.qxd 8/14/2003 8:09 AM Page 23 System Operation 5.5 RUNNING A PROGRAM To start a program (be sure to enter the Program mode by using the Mode key), press the Run key. This will start the run. By using the right soft key, the Run screen can show time elapsed or time remaining on the run. If program steps are in volume, elapsed or remaining volume will also be displayed.
4006161B.qxd 8/14/2003 8:09 AM Page 24 System Operation 5.5.1 Holding a Run Pressing the Hold key displays the following screen: Holding CONTINUE ___%B Abort When holding, the pump runs indefinitely at the current programmed conditions. Selecting Continue will continue the run. Selecting Abort will stop the run, leaving the pump in Program mode, and indicate, by the Step LED, which step was running at the time Abort was pressed. 5.5.
4006161B.qxd 8/14/2003 8:09 AM Page 25 System Operation 5.5.4 Power Failure While in Run Mode In the event of a power failure while a Program is running, the LCD display will indicate: Power Failure! CONTINUE stop If Stop is selected, the program that was running is aborted. Pressing any key on the front panel will display the Manual mode. If Continue is selected, the program that was running is resumed at the point where the power failure occurred.
4006161B.qxd 8/14/2003 8:09 AM Page 26 Other Instruments 6.0 OPERATION WITH OTHER BIO-RAD INSTRUMENTS The Econo Gradient Pump (EGP) may be used with other chromatography components (the Model 2110 or Model 2128 fraction collectors and the Model 1327 Chart recorder), and with the BioLogic Duo-Flow System running software version 3.0 or later, the high resolution liquid chromatography systems. Each of these connections is discussed in the following table.
4006161B.qxd 8/14/2003 8:09 AM Page 27 Other Instruments Model 2128 Fraction Collector (continued) Alternatively, one may use the Model 2128 Diverter Valve; it will respond to the program in the Model 2128 Fraction Collector itself, not from the EGP. If HOLD is selected during the program, then the pump will continue pumping at the current conditions and fraction collection will continue as programmed. If PAUSE is selected during the program, then the pump and fraction collector will stop.
4006161B.qxd 8/14/2003 8:09 AM Page 28 Other Instruments Model 2110 Fraction Collector (continued) When a programmed run is started, the EGP will automatically start the fraction collector. If the SV-3 diverter valve is connected to the EGP, then fraction collection will begin at the point in the program when the diverter valve is in the FRAC position.
4006161B.qxd 8/14/2003 8:09 AM Page 29 Other Instruments Model 1327 Chart Recorder (continued) If PAUSE is selected during the program, then the pump and chart recorder will stop. The pump and chart recorder will resume when CONTINUE is selected. Model EM-1 UV Monitor None A connection is not required between the pump and the EM-1 UV Monitor. To record UV data onto the Model 1327 Chart Recorder, use cable #4, 8-pin mini-DIN to banana plug cable. It is supplied with the UV Monitor.
4006161B.qxd 8/14/2003 8:09 AM Page 30 Other Instruments BioLogic Duo-Flow System One of these four System (bus) Cables: The EGP can be controlled by the BioLogic Duo-Flow System provided that the BioLogic System firmware is version 3.0 or higher. Cable #17 4-ft (1.2m) (Catalog # 750-0650) Connect the EGP to the Duo-Flow Workstation using a bus cable of the appropriate length. Cable #18 12-ft (3.7m) (Catalog # 750-0651) Cable #19 30-ft (9.
4006161B.qxd 8/14/2003 8:09 AM Page 31 Other Instruments BIOLOGIC DUO-FLOW WORKSTATION ECONO GRADIENT PUMP MODEL 1327 CHART RECORDER GRADIENT MIXER CABLE 22 OR 23 DIVERTER OR SPLITTER VALVE MIXER B A MODEL 2110 FRACTION COLLECTOR OR MODEL 2128 FRACTION COLLECTOR Figure 6-1. System Cabling.
4006161B.qxd 8/14/2003 8:09 AM Page 32 Other Instruments ECONO GRADIENT PUMP MIXER B A GRADIENT MIXER ECONO GRADIENT PUMP MODEL EM-1 UV OPTICS MODULE MODEL 2128 FRACTION COLLECTOR MODEL EG-1 GRADIENT MONITOR MODEL 2110 FRACTION COLLECTOR Figure 6-2. System Plumbing.
4006161B.qxd 8/14/2003 8:09 AM Page 33 Maintenance 7.0 CLEANING AND MAINTENANCE The Econo Gradient Pump requires very little maintenance to assure reliable operation. The procedures outlined below will insure maximum pump life: • Check tubing regularly for signs of cracking and wear. If any exist, replace the tubing. • For optimal tubing life, use a slow to medium pump speed. To increase throughput, increase tubing size to obtain the desired output.
4006161B.qxd 8/14/2003 8:09 AM Page 34 Maintenance 4. The fuse socket in the fuse drawer is removable. Turn the socket such that the correct voltage shows through the window. 5. Reinsert the fuse drawer into the power entry module, with the locking tab to the left. Press gently until it snaps into place. 6. Insert the power cord plug into the power entry module. Plug the power cord into a properly grounded outlet. 110 220 Power Entry Module Voltage Selector Fuse Drawer Window Figure 7-1.
4006161B.qxd 8/14/2003 8:09 AM Page 35 Troubleshooting 8.0 TROUBLESHOOTING Problem Possible Cause Solution Pump displays Power outage “Power Failure!” If you wish to continue a run in progress, press the Continue soft key. The LCD will display the program at the time of the power failure. Pressing the Stop soft key will display the message “Program Aborted”. You may now operate the pump as normal. Pump will not run Verify that power cord is plugged in and the power switch is turned on.
06161B.qxd 8/14/2003 8:09 AM Page 36 Troubleshooting Problem Flow rate not consistent Possible Cause Solution Pump tubing damaged or worn out Replace tubing. Platen adjustment Adjust the platen by turning incorrect it counter clockwise until it is completely loosened, then rotate three to five full turns clockwise. See section 4.3, Platen Adjustment. Wrong tubing size Refer to Figure 4-1 for selection selected criteria. Select proper ID tubing within Setup.
4006161B.qxd 8/14/2003 8:09 AM Page 37 Troubleshooting Problem Cannot calibrate pump Valves do not respond or actuate Possible Cause Solution Pump must be Insure that pump is stopped. stopped in order to calibrate pump RPM has been selected Select Flow Rate in screen 1 of Setup. Pump is off Turn on the pump. Valve cabled to wrong connector Mixer and/or Aux valve must be plugged in to its correct socket.
4006161B.qxd 8/14/2003 8:10 AM Page 38 Embedded Functions 9.0 EMBEDDED FUNCTIONS During a technical service call, you may be asked to perform tasks which require you to press combinations of keys. These tasks are defined below. 9.1 FIRMWARE CONFIRMATION To check the version of firmware installed in the Econo Gradient Pump, power up the Econo Gradient Pump. During power up, the firmware version is displayed. The checksum (CS) is also displayed. 9.
4006161B.qxd 8/14/2003 8:10 AM Page 39 Specifications APPENDIX A TECHNICAL SPECIFICATIONS Number of channels 2 Flow rate range (per channel) 0.002 - 20 ml/min (depending on tubing diameter) Pump head speed 0.05 to 25 rpm maximum Tubing diameter 0.19 mm ID to 3.2 mm ID; maximum 1 mm wall thickness Speed adjustment increment 0.01 RPM Speed stability 0.2% at 10 RPM Speed accuracy 0.
4006161B.qxd 8/14/2003 8:10 AM Page 40 Specifications Gradient Mixer Range 0-100% B Increment 1% B Valve control SV-3 Diverter Valve Waste and divert positions.
4006161B.qxd 8/14/2003 8:10 AM Page 41 Other Instruments APPENDIX B CONNECTION TO OTHER INSTRUMENTS The I/O connector on the rear panel of the pump is for the operation of the fraction collectors and chart recorder (see Figure 3-2). Output signals are TTL compatible. To use these signals for non-Bio-Rad devices, you must insure that the circuit external to the Econo Gradient Pump does not draw more than 15 milliamperes of current.
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4006161B.qxd 8/14/2003 8:10 AM Page 43 Warranty APPENDIX C WARRANTY The Econo Gradient Pump is warranted for one year against defects in materials and workmanship. If any defects should occur during this warranty period, Bio-Rad will replace the defective parts without charge. However, the following defects are specifically excluded: 1. Defects caused by improper operation. 2. Repair or modification done by anyone other than Bio-Rad Laboratories or their authorized agent. 3.
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4006161B.qxd 8/14/2003 8:10 AM Page 45 Ordering Information Catalog Number Product Description Tubing and Accessories 731-8210 Silicone Tubing, 0.8 mm ID, 0.8 mm wall, 10 m 731-8211 Silicone Tubing, 1.6 mm ID, 0.8 mm wall, 10 m 731-8212 Silicone Tubing, 3.2 mm ID, 0.8 mm wall, 10 m 731-8213 Tygon tubing, 0.51 mm ID, 0.8 mm wall, 10 m 731-8214 Tygon Tubing, 0.8 mm ID, 0.8 mm wall, 10 m 731-8215 Tygon Tubing, 1.6 mm ID, 0.8 mm wall, 10 m 731-8207 PharMed Tubing, 0.8 mm ID, 1.
4006161B.qxd 8/14/2003 8:10 AM Page 46 Ordering Information Catalog Number 731-9006 Product Description Low Pressure, Small Fittings Kit. Contains a subset of the fittings found in catalog # 731-8220, including barbto-female and male luer for 0.8, 1.6, and 3.2 mm tubing, slip fittings, and both female and male luer plugs. Cables 731-8264 System Cable 4, 8-pin mini-DIN to banana cable. To connect Econo UV Monitor or Econo Gradient Monitor to the Model 1327 Chart Recorder.
4006161B.qxd 8/14/2003 8:10 AM Page 47 Bio-Rad Laboratories Life Science Group Bulletin 0000 US/EG Web site www.bio-rad.