M7318120G.
M7318120G.qxd 10/21/2002 2:47 PM Page ii Warranty Model _________________________________________ Serial No. ______________________________________ Date of Delivery_________________________________ Warranty Period _________________________________ Unless otherwise specified, instruments sold by Bio-Rad Laboratories are under warranty for 1 year against defects in materials and workmanship. If any defects should occur during this warranty period, Bio-Rad will replace the defective parts without charge.
M7318120G.qxd 10/21/2002 2:47 PM Page iii Table of Contents Section 1 Safety .............................................................................. 1 Section 2 Introduction ................................................................... 2 Section 3 Unpacking and Voltage Conversion ............................ 3.1 3.2 Unpacking Instructions ............................................................ Voltage Conversion .................................................................
M7318120G.qxd 10/21/2002 2:47 PM Page iv Appendix B Model 2110 Fraction Collector Procedure for Adjusting Drop Detector Signal Level .................. 22 Appendix C Ordering Information ...................................................
M7318120G.qxd 10/21/2002 2:47 PM Page 1 Section 1 Safety ! Disconnect supply before servicing. No user serviceable parts inside, refer servicing to Bio-Rad service personnel. ! High leakage current, ensure proper grounding. This instrument is intended for laboratory use only. This product conforms to the “Class A” standards for electromagnetic emmissions intended for laboratory equipment applications.
M7318120G.qxd 10/21/2002 2:47 PM Page 2 Section 2 Introduction Fig. 2.1. Model 2110 Fraction Collector. The Model 2110 Fraction Collector (Figure 2.1), shown with the optional instrument dust cover, is designed to be used with gravity feed columns, preparative electrophoresis and liquid chromatography systems. The Model 2110 Fraction Collector will accurately collect up to 80 fractions, at a flow rate of up to 40 ml/min.
M7318120G.qxd 10/21/2002 2:47 PM Page 3 carousel where tube changes are initiated upon reaching a predetermined drop count (1-999 drops) or time interval (0.05-99.9 minutes), or upon command from an external source. The silicone drop former insures uniform drop size for accurate drop count measurement. The drop former will accommodate either 1¼8-inch or 1 ¼16-inch inlet tubing, without the need for additional fittings. The Model 2110 Fraction Collector is spill-proof and solvent resistant.
M7318120G.qxd 10/21/2002 2:48 PM Page 4 4. Reinsert the fuse drawer in the power entry module, with the locking tab to the left. The voltage indicator will read right-side-up if the drawer is oriented correctly. Push the fuse drawer in gently until it snaps into place. 5. Connect the power cord to the power entry module and power mains. 6. There is no power switch on the fraction collector. When connected to an AC power source, the Model 2110 Fraction Collector will come on in the Stop or Set mode.
M7318120G.qxd 10/21/2002 2:48 PM Page 5 Section 4 Physical Description and Control Features 4.1 Physical Description Inlet tubing Arm cap Drop former Drop arm Drive seal Carousel positioning guides Drive gear Pivot shaft Slider Fig 4.1. Model 2110 Fraction Collector features. Feature Drop arm Function Directs flow to the collection tubes. • Collection position, rotated forward, the drop arm is directly above each collection tube.
M7318120G.qxd 10/21/2002 2:48 PM Page 6 Feature Carousel Function Holds the collection tubes. The tube positions are numbered 1-80 on the flange of the carousel, and line up with the center of each tube. The carousel is designed so that any liquid spilled in it will flow to the center and out the rear of the base. The carousel may also be used for storage of test tubes. Drop former Seals against the inlet tubing (1¼16" or 1¼8" OD) to form uniform size drops. Arm cap Holds the drop former in place.
M7318120G.qxd 10/21/2002 2:48 PM Page 7 4.2 Front Panel Functions MODEL 2110 FRACTION COLLECTOR Fig. 4.2. Front panel controls. ml min ml/min % Feature Run/Stop key Function The Run/Stop key is used to select standby or active mode. In the active mode, the collector will collect by time or drops, or accept an external signal. When the collector is controlled by a system, both the standby and active mode lights will be lit, and the LED display will read “---”.
M7318120G.qxd 10/21/2002 2:48 PM Page 8 Fig. 4.3 Rear Panel connections. 4.3 Rear Panel Functions Connection Power entry module Function Grounded 3-pin receptacle for the power cord. Also contained within is a four-position 100 V/120 V/ 220 V/ 240 V line voltage selector. The collector is shipped in its 120 V or 220 V version. For operation at other voltages, refer to Section 3.2. 9-pin “D” connector For remote operation of the collector.
M7318120G.qxd 10/21/2002 2:48 PM Page 9 Section 5 Instrument Set-Up 5.1 Tubing Installation 1. Insert the drop former, narrow end downward, into the drop arm, pressing it firmly in the hole (see Figure 4.1). 2. Using a twisting motion, press the arm cap onto the drop arm to hold the drop former in place. 3. Insure that the end of the inlet tubing is cleanly cut and not flattened or deformed. Insert tubing into the drop former, pushing down on the tubing until the drop former starts to stretch.
M7318120G.qxd 10/21/2002 2:48 PM Page 10 a b 2 c Fig. 5.1. Carousel installation.
M7318120G.qxd 10/21/2002 2:48 PM Page 11 5.2 Carousel Installation The Model 2110 Fraction Collector fills tubes from the outside of the carousel inward. Load as many tubes as desired into the carousel, starting at the outside end of the spiral and moving inward. The carousel need not be fully loaded, since any eluant flowing after the last tube will drain through the carousel into the well in the case and out the central drain tube located at the rear of the instrument. 1.
M7318120G.qxd 10/21/2002 2:48 PM Page 12 collection tube, and the eluant will flow through the carousel to waste out the rear of the collector. 5.4 Waste Collection Port All spills on the top of the case and carousel, as well as waste eluant within the carousel, drain to a central waste-collection port at the rear of the instrument case. From this port, a drain tube passes out through the rear of the case.
M7318120G.qxd 10/21/2002 2:48 PM Page 13 Drop Collection Mode The fraction collector counts drops at flow rates up to ~5 ml/min. Drop counting is performed by the sensor in the drop arm. If the flow rate exceeds 5 ml/ min., counting will not be accurate, and use of the Time mode is recommended. 1. Plug the Model 2110 Fraction Collector into a grounded wall outlet. The collector will power up in the Stop, or Standby mode, with the LED lit. 2. Press the Time/Drop key until the lit. 3.
M7318120G.qxd 10/21/2002 2:48 PM Page 14 Time Mode The fraction collector collects fractions at intervals of .01 minutes, for times per tube in the range of .05 to 9.99 minutes, and at.10 minute intervals for times per tube in the range of 10.0 to 99.9 minutes. 1. Press the Run/Stop key until the 2. Press the Time/Drop key until the ml min ml/min % LED is lit. LED is lit. 3. Select the time interval per tube by pressing the keys. The LED multi-display will display the time interval selected. 4.
M7318120G.qxd 10/21/2002 2:48 PM Page 15 1. Attach the stopcock into the hole in the top of the drop arm. Position the stopcock so that its handle overhangs the end of the arm and is free to turn. 2. Attach the column to the stopcock, making sure both stopcock and column are firmly connected. 6.4 Manual Advance The Model 2110 Fraction Collector may be manually advanced to the next tube by pressing the key. This key is functional in both Stop/Standby and Run modes.
M7318120G.qxd 10/21/2002 2:48 PM Page 16 7.2 Remote Operation with BioLogic LP Use System Cable 1 (catalog number 731-8261) to connect the BioLogic LP to the fraction collector. Connect the 9-pin “D” connector to rear of the Model 2110, and the mini-DIN plug to the rear port of the BioLogic LP controller in the port marked FRACTION COLLECTOR. (See BioLogic LP manual if further explanation is required.
M7318120G.qxd 10/21/2002 Pin 6 7 8 9 2:48 PM Page 17 Function External Start Advanced Contact 1 Advanced Contact 2 Logic Ground Signal Type Input Output Output External Advance (Pin 1) The External Advance line performs two functions. It causes the instrument to enter the Remote mode when held low for longer than two seconds, and it causes the carousel to advance one position each time a high-going pulse is applied.
M7318120G.qxd 10/21/2002 2:48 PM Page 18 External Start The external start line is used to allow another instrument to remotely start and stop the fraction collector’s programmed functions. Applying a low pulse of at least 100 msec to pin 6 has the same effect as pressing the Run/Stop key on the front panel. That is, the first pulse will start the previously selected mode (drops or time) and the second pulse will stop the program.
M7318120G.qxd 10/21/2002 2:48 PM Page 19 Section 9 Cleaning and Maintenance The Model 2110 Fraction Collector requires very little maintenance to insure reliable operation. Two procedures are outlined below. 9.1 Drop Sensor The Model 2110 Fraction Collector counts drops through an optical sensor located in the drop area inside the drop former arm. Precipitates or other foreign debris may obscure the optical path and cause erroneous drop counts.
M7318120G.qxd 10/21/2002 2:48 PM Page 20 Fraction Collector Error Codes There are several self-diagnostics built into the software of the Model 2110 Fraction Collector. Below are the principal error numbers and codes which occur. If other error codes appear, try unplugging and replugging the instrument, or call Bio-Rad Technical Service.
M7318120G.qxd 10/21/2002 2:48 PM Error Number E00 E01 E04 Page 21 Cause Hardware failure E02 Motor jam E08 E10 Drop sensor Remedy Unplug and replug to mains power. If error persists, contact your local Bio-Rad representative. Reposition carousel; unplug and replug to mains power. If problem persists, apply WD-40 to the motor shaft. If motor must be replaced, order part number 8006484.
M7318120G.qxd 10/21/2002 2:48 PM Page 22 Operating temperature + 4 to + 40 °C Storage temperature - 5 to + 71 °C Line voltage 90-132 VAC or 180-265 VAC, 47 Hz to 63 Hz Power consumption Fuse rating 100,120 V Operation 25 VA max, 15 W max, fused 200 mA, 250 V, 5 x 20 mm, Fast Acting, UL/CSA approved Ref: Schuter 034.3923 or Bussmann GMA200mA 220,240 V Operation 100 mA,250 V, 5 x 20 mm, Time Lag (FST), VDE approved Ref: Schuter 034.3107 Size 24.0 x 33.0 x 25.
M7318120G.qxd 10/21/2002 2:48 PM Page 23 Tools required: Small phillips-head screwdriver, small slot-head screw driver. Procedure: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Disconnect the Model 2110 collector from the power source. Disconnect any tubing leading to the fraction collector, remove the yellow plastic arm cap, white rubber drop former, and carousel. Clean the optical window and light source (located in drop arm), using the a cotton swab moistened with water.
M7318120G.
M7318120G.qxd 10/21/2002 2:48 PM Page 25 Bio-Rad Laboratories Life Science Group Bulletin 0000 US/EG Web site www.bio-rad.
