M7318140J.qxp 4/17/2006 2:17 PM Page a Model EP-1 Econo Pump Instruction Manual Catalog Numbers 731-8140 731-8142 For Technical Service Call Your Local Bio-Rad Office or in the U.S.
M7318140J.qxp 4/17/2006 2:17 PM Page b Warranty Model _________________________________________ Serial Number ___________________________________ Date of Delivery _________________________________ Warranty Period _________________________________ The Model EP-1 Econo Pump is warranted for 1 year against defects in materials and workmanship. If any defects should occur during this warranty period, Bio-Rad will replace the defective parts without charge.
M7318140J.qxp 4/17/2006 2:17 PM Page c Table of Contents Section 1 Safety ..............................................................................1 Section 2 Introduction ...................................................................2 Section 3 Unpacking and Setting Up............................................3 3.1 3.2 Unpacking Instructions.............................................................3 Voltage Conversion................................................................
M7318140J.qxp 4/17/2006 2:17 PM Page 1 Section 1 Safety ! Disconnect supply before servicing. No user serviceable parts inside, refer servicing to Bio-Rad service personnel. This instrument is intended for laboratory use only. This product conforms to the “Class A” standards for electromagnetic emissions intended for laboratory equipment applications.
M7318140J.qxp 4/17/2006 2:17 PM Page 2 Section 2 Introduction Fig. 2.1. Model EP-1 Econo Pump. The Model EP-1 Econo Pump is a two-channel, bi-directional, variable speed peristaltic pump for low-pressure chromatography and general laboratory use. It offers a full range of features to facilitate ease of use as a stand-alone pump or as a part of the BioLogic System. As a stand-alone pump, the Model EP-1 Econo Pump delivers flow rates from 0.
M7318140J.qxp 4/17/2006 2:17 PM Page 3 Section 3 Unpacking and Setting Up 3.1 Unpacking Instructions Carefully remove the unit from the shipping box, lifting from the handle on the back of the pump or from the bottom of the instrument. Remove the plastic bag and inspect the instrument for any external damage. Parts included with the Model EP-1 Econo Pump are illustrated in Figure 3.1. Check off all parts against the supplied packing list.
M7318140J.qxp 4/17/2006 2:17 PM Page 4 3.2 Voltage Conversion Warning: The Model EP-1 Econo Pump is shipped in its 120 V or 220 V version. To operate at other voltages, refer to the procedure below. Failure to follow this procedure may result in damage to the unit and invalidation of the warranty. Prior to connecting the power cord to the power entry module and wall outlet, verify that the voltage indicated on the power entry module matches your line voltage.
M7318140J.qxp 4/17/2006 2:17 PM Page 5 Section 4 Physical Description and Control Features 4.1 Front Panel Functions ECONO PUMP ml min ml/min % 0.8 1.6 Vo Vt 3.2 CAL Fig. 4.1. Front panel controls. Display Function LED Display The four-character display will show various parameters during programming and operation of the Econo Pump. The indicator lights located immediately to the right of the display indicate the units displayed: ml, minutes, ml/minute, or percent.
M7318140J.qxp 4/17/2006 ml 2:17 PM Page 6 Indicator Lights These lights indicate the units for the value displayed on the LED display located to the left. A flashing light indicates that a value can be set using the Arrow keys. Run/Stop Key This key is used to start or stop the pump. It also has secondary functions such as holding or aborting a method. Purge Key This key is used to prime and purge tubing lines without disturbing the primary speed setting of the pump.
M7318140J.qxp 4/17/2006 2:17 PM Page 7 Program Run Key This key is used to control a programmed gradient or fraction collection method. When the indicator light is flashing, pressing this key starts the method. When the indicator light is lit, pressing this key will interrupt the program, which can be continued by pressing again. When the LED displays “FAIL”, (indicating a brief power outage has occurred), pressing this key continues the program.
M7318140J.qxp 4/17/2006 2:17 PM Page 8 Diverter Valve Output This 8-pin outlet sends a signal to the Model SV-3 diverter valve, to divert flow between collection devices. Auxiliary Output This 8-pin connector is for communication with the Model EM-1 Econo UV Monitor and the BioLogic System. 4.3 Pump Base Features The base of the Model EP-1 Econo Pump contains a 40-pin connector for connection of the Econo Pump to the (discontinued) Econo System Controller.
M7318140J.qxp 4/17/2006 2:17 PM Page 9 When using tubing other than precut tubing from Bio-Rad, the tubing must be cut to the following lengths to accommodate prestretch: Tubing Length Tygon, PharMed 179 mm +/- 1.5 (7.04" +/- 0.05) Silicone 171 mm +/- 1.5 (6.75" +/- 0.05) Install tubing onto the pump head as described in Section 5.2. ! Warning: If using tubing other than the type supplied by Bio-Rad, make sure the wall thickness is not greater than 1.0 mm.
M7318140J.qxp 4/17/2006 2:17 PM Page 10 Lock-Ring Color Tubing ID Red Orange Yellow 0.8 mm (1/32") 1.6 mm (1/16") 3.2 mm (1/8") Fig. 5.2. Attachment of tubing fittings. 3. Insert one end of the tubing into the tubing retaining bracket of the pump head. Lightly pull the tubing around the rollers to remove slack. Attach the other end of tubing into the tubing retaining bracket on the opposite side of the pump head. 4.
M7318140J.qxp 4/17/2006 2:17 PM Page 11 Note: Overtightening the platen adjustment screw will reduce flow rate and shorten tubing life. If the platen is too loose, flow rate will decrease as backpressure increases. Section 6 Stand Alone Operation The Model EP-1 Econo Pump functions as a stand-alone pump. By specifying the pump tubing size, it allows you to enter a flow rate rather than a nominal pump speed setting. (An uncalibrated Econo Pump will display pump speed as percent of maximum rpm.
M7318140J.qxp 4/17/2006 0.8 2:17 PM 3. Press Calibration key until “CAL” is displayed. Press the Run/Stop key. Pump will run for 5 minutes at 25% of maximum speed, and the display will count down time. 4. When pump stops, read the volume of fluid on the graduated cylinder. Using the Arrow keys, enter this amount on the display. Alternatively, when a suitable volume of liquid has been collected, pressing Run/Stop will turn off the timer and stop the pump. Enter volume collected as described. 5.
M7318140J.qxp 4/17/2006 2:17 PM Page 13 7.1 Use of the Econo Pump with the Model 2110 Fraction Collector The Econo Pump is capable of controlling the Model 2110 Fraction Collector. The pump must be calibrated in order to run a fraction collector program. See Section 6 for calibration instructions. Use System Cable 1 (catalog number 731-8261) to connect the Econo Pump to the fraction collector.
M7318140J.qxp 4/17/2006 2:17 PM 4. After selecting a Vt value, press the flashing Fraction Collection key to enter the value. The flashing LED display shows the estimated number of fractions to be collected. 5. Press the flashing Fraction Collection key once more to enable the fraction collector. To actuate the fraction collector, press the Program Run key after starting the pump. (The fraction collector can be programmed with the pump running or stopped.
M7318140J.qxp 4/17/2006 2:17 PM Page 15 2. During the Vo period of the fraction collection scheme (as indicated by the Vo indicator light), the fraction collector is not active. To collect fractions during this void volume period, cancel Vo by pressing the Fraction Collector key. The fraction collector will start, with the LED display indicating the time-progression of the collection scheme. 3.
M7318140J.qxp 4/17/2006 2:17 PM Outlet to Waste Page 16 Inlet Outlet to Collector Fig. 7.1. Connection of the Model SV-3 Diverter Valve. 7.2 Use of the Econo Pump with the Model 2128 Fraction Collector The Econo Pump is capable of controlling the Model 2128 Fraction Collector. The pump must be calibrated in order to run a fraction collector program. See Section 6 for calibration instructions. Use System Cable 15 (catalog number 731-8286) to connect the Econo Pump to the Model 2128 Collector.
M7318140J.qxp 4/17/2006 2:17 PM Page 17 7.3 Use of the Econo Pump with the BioLogic System The Econo Pump can be controlled by the BioLogic System and used as a sample loading pump. Note: The Econo Pump must have v2.12 software in order to accept on/off commands from the BioLogic System. See Section 10.1 for instructions on how to check the software version. If an upgrade is required, contact your local Bio-Rad representative.
M7318140J.qxp 4/17/2006 2:17 PM Page 18 Section 9 Troubleshooting Problem Possible Cause Solution Pump displays "OUT" Power surge or brownout 1. Turn the power switch off. 2. Press and hold down the down arrow key and the CAL key simultaneously while turning the power switch on. This will reset the pump. If the pump was previously calibrated you will have to recalibrate it. Pump displays "FAIL" Power outage If you wish to continue a run in progress, press program run key.
M7318140J.qxp 4/17/2006 Problem 2:17 PM Page 19 Possible Cause Solution Large change in back pressure or fluid viscosity Re-calibrate flow. Restriction on inlet side Remedy Excessive tubing wear Platen too tight Turn platen adjustment screw clockwise to stop flow, then clockwise until flow starts. Cannot calibrate pump Pump must be stopped in order to calibrate pump Insure that pump is stopped.
M7318140J.qxp 4/17/2006 2:17 PM Page 20 Appendix A Technical Specifications Number of channels 2 Flow rate range (per channel) 0.1-20 ml/min (depending on tubing diameter) Pump head speed 25 rpm maximum Tubing diameter 0.4 mm ID to 3.
M7318140J.qxp 4/17/2006 2:17 PM Page 21 Appendix B Operation of the Model EP-1 Econo Pump When Connected to Other Instruments The Model EP-1 Econo Pump provides a variety of output signals which can be used to control the operation of non Bio-Rad fraction collectors, or to communicate with non Bio-Rad UV monitors and recording devices. Communication is accomplished through two standard 8-pin mini-DIN sockets on the rear panel of the pump.
M7318140J.qxp 4/17/2006 2:17 PM Page 22 Appendix C Ordering Information Catalog Number Product Description Model EP-1 Econo Pump 731-8140 Model EP-1 Econo Pump, 110 V (USA power cord), with tubing set and starter fittings kit 731-8142 Model EP-1 Econo Pump, 220 V (no power cord), with tubing set and starter fittings kit 731-8145 Model EP-1 Pumphead Assembly 731-8235 Model SV-3 Diverter Valve Tubing and Accessories 731-8210 Silicone Tubing, 0.8 mm ID, 0.
M7318140J.qxp 4/17/2006 Catalog Number 731-8220 2:17 PM Page 23 Product Description Low Pressure Fittings Kit, includes over 250 male and female luer connectors, 2- and 3-way stopcocks, and tubing connectors Cables 731-8261 System Cable 1, 8-pin mini-DIN to DB-9 connector. For connection of Model 2110 Fraction Collector to the Econo Pump, to the BioLogic LP 731-8262 System Cable 2, 8-pin mini-DIN to 8-pin standard DIN.
M7318140J.qxp 4/17/2006 2:17 PM Page 24 Bio-Rad Laboratories Life Science Group Bulletin 0000 US/EG Web site www.bio-rad.
