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Kaleidoscope Prestained

Standards

Catalog Number

161-0324

Product shipped at room temperature.

Store at -20 °C upon arrival.

Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547

LIT599 Rev E

Reference

1. Laemmli, U. K., Nature, 227, 680 (1970).

Ordering Information

Catalog

Number Product Description

Prestained Standards

161-0324 Kaleidoscope Prestained Standards, 500 µl

161-0325 Kaleidoscope Polypeptide Standards, 500 µl

161-0305 Prestained SDS-PAGE Standards, low range, 500 µl

161-0309 Prestained SDS-PAGE Standards, high range, 500 µl

161-0318 Prestained SDS-PAGE Standards, broad range, 500 µl

Molecular Weight Standards

161-0326 Polypeptide SDS-PAGE Standards, 200 µl

161-0304 SDS-PAGE Standards, low range, 200 µl

161-0303 SDS-PAGE Standards, high range, 200 µl

161-0317 SDS-PAGE Standards, broad range, 200 µl

161-0314 Silver Stain SDS-PAGE Standards, low range, 200 µl

161-0315 Silver Stain SDS-PAGE Standards, high range, 200 µl

161-0306 Biotinylated SDS-PAGE Standards, low range, 250 µl

161-0311 Biotinylated SDS-PAGE Standards, high range, 250 µl

161-0319 Biotinylated SDS-PAGE Standards, broad range, 250 µl

161-0320 2-D SDS-PAGE Standards, 500 µl

IEF Standards

161-0310 IEF Standards, pI range 4.45-9.6, 250 µl

Fig. 1. Kaleidoscope Prestained

Standards. 5 µl and 10 µl of the stan-

dards were run on a 4-20% Ready Gel

according to the method of Laemmli.

The gels were run on the Mini-

PROTEAN

II cell and dried using the

Model 583 Gel Dryer.

Fig. 2. Kaleidoscope Prestained

Standards and Prestained SDS-PAGE

Standards. Prestained standards were

run on a 4-20% Ready Gel in the Mini-

PROTEAN II cell according to the

method of Laemmli.

The standards

were then electrophoretically transferred

to nitrocellulose using a Mini Trans-Blot

cell. Lane 1, 5 µl high range Prestained

SDS-PAGE Standards; Lane 2 and 3,

5 µl Kaleidoscope Prestained Standards;

Lane 4, 5 µl low range Prestained SDS-

PAGE Standards.

1234

Myosin

ß-galactosidase

Bovine serum albumin

Carbonic anhydrase

Soybean trypsin inhibitor

Lysozyme

Aprotinin

LIT599E 5/27/99 8:06 AM Page 1

Summary of content (3 pages)

PAGE 1
5/27/99 8:06 AM Page 1 1 LIT599E 2 Fig. 2. Kaleidoscope Prestained Standards and Prestained SDS-PAGE Standards. Prestained standards were run on a 4-20% Ready Gel in the MiniPROTEAN II cell according to the method of Laemmli. 1 The standards were then electrophoretically transferred to nitrocellulose using a Mini Trans-Blot® cell. Lane 1, 5 µl high range Prestained SDS-PAGE Standards; Lane 2 and 3, 5 µl Kaleidoscope Prestained Standards; Lane 4, 5 µl low range Prestained SDSPAGE Standards. Fig. 1.
PAGE 2
LIT599E 5/27/99 8:06 AM Page 1 1 Fig. 1. Kaleidoscope Prestained Standards. 5 µl and 10 µl of the standards were run on a 4-20% Ready Gel according to the method of Laemmli. 1 The gels were run on the MiniPROTEAN® II cell and dried using the Model 583 Gel Dryer. 2 Fig. 2. Kaleidoscope Prestained Standards and Prestained SDS-PAGE Standards. Prestained standards were run on a 4-20% Ready Gel in the MiniPROTEAN II cell according to the method of Laemmli.
PAGE 3
LIT599E 5/27/99 8:06 AM Page 2 Constituent Proteins (See enclosed insert for lot specific calibrated molecular weights.)† Protein Myosin ß-galactosidase Bovine serum albumin Carbonic anhydrase Soybean trypsin inhibitor Lysozyme Aprotinin † Protein Color Blue Magenta Green Violet Orange Red Blue Covalently bound dye alters the molecular weight of the proteins and produces relatively broad bands. The molecular weights are calibrated from the center of each protein band.