Instruction Manual

2 | Automated Droplet Generator
QX200 AutoDG Droplet Digital PCR System
The ddPCR System lets you:
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Achieve absolute quantification without the use of a standard curve
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Detect rare DNA target copies with unmatched sensitivity
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Determine copy number variation with unrivaled accuracy
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Measure gene expression levels with precision
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Design scalable assays for high sensitivity or high throughput
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Expand applications using flexible ddPCR chemistry — EvaGreen or probes
Applications and uses include:
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Copy number variation
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Rare sequence detection
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Gene expression analysis
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Next-generation sequencing (NGS) library
quantification
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Viral load determination
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Single cell gene expression analysis
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Absolute quantification
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Rare mutant detection
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miRNA analysis
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NGS sample preparation
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GMO detection
1.2 System Components
Bio-Rad’s Automated Droplet Generator simplifies the Droplet Digital PCR workflow, making digital PCR both
scalable and practical. The AutoDG Instrument prepares droplets for up to 96 samples at a time with minimal
hands-on time required. In less than 45 minutes, a plate of droplets can be generated. The plate can then be
sealed, thermal cycled, and ddPCR analysis can be performed on either a QX100
™
or QX200 Droplet Reader.
Automated droplet generation eliminates user-to-user variability that can be associated with manual droplet
generation. The AutoDG Instrument’s HEPA-filtered enclosure reduces contamination during droplet
generation. Consumables are automation-friendly with SBS standard access.
This manual covers use of the Automated Droplet Generator and preparation for PCR. For information on the QX200
Droplet Reader, please refer to the QX200 Droplet Reader Instruction Manual, part number 10031906.