Instruction manual

QX200 Droplet Generator Instruction Manual QX200 Droplet Generator Instruction Manual | 3
Chapter 1 QX200 Droplet GeneratorChapter 1 QX200 Droplet Generator
Table 1.2. Additional materials required for droplet generation.
Component Recommended Catalog #
Reagents for probe detection
PCR supermix ddPCR supermix for probes (no dUTP) 186-3023, 186-3024, 186-3025
ddPCR supermix for probes 186-3010, 186-3026, 186-3027,
186-3028
One-Step RT-ddPCR supermix for probes 186-3021, 186-3022
Droplet generation oil Droplet generation oil for probes 186-3005
Control ddPCR buffer control kit for probes 186-3052
Reagents for EvaGreen detection
PCR supermix QX200
™
ddPCR
™
EvaGreen
®
supermix 186-4033, 186-4034, 186-4035,
186-4036
Droplet generation oil Droplet generation oil for EvaGreen 186-4005, 186-4006
Control QX200 buffer control kit for EvaGreen 186-4052
Consumables and other materials
Pipets 20 μl pipet for sample loading Rainin L-20
50 μl pipet for droplet transfer Rainin L-50, L8-50
8-channel, 200 μl pipet for oil Rainin L8-200
Pipet tips Filtered Rainin GP-L10F, GP-L200F
96-well PCR plates twin.tec semi-skirted 96-well plate Eppendorf 951020362
Reagent trough Any
Foil plate seals Pierceable foil plate seals 181-4040
Plate sealer PX1
™
PCR plate sealer 181-4000
8-cap strips Any
1.3 Installation and General Operation

Connect the QX200 droplet generator to a power source using only the power cord and power
adapter provided. Ensure the ground is reliably connected before plugging in the instrument. Position
the instrument such that it can be easily disconnected from the power source should that become
necessary for servicing the equipment.

Leave 10" (25.4 cm) clear space behind and 5" (12.7 cm) clear to the right and left for proper ventilation

Power on the droplet generator by plugging it in. The status indicator turns solid green to indicate power is on

Open and close the instrument by pressing the button on top of the green lid
U.S. standard power cord set with grounded plug
(Type 5-15P) and C5 connector (10 A/125 V)
Power supply to 5 mm DC power jack inlet