Instruction manual

QX200 Droplet Generator Instruction Manual QX200 Droplet Generator Instruction Manual
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Chapter 1 QX200 Droplet GeneratorChapter 1 QX200 Droplet Generator
Table 1.2. Additional materials required for droplet generation.
Component
R
ecommended
C
atalog #
R
eagents for probe detection
PCR supermix
d
dPCR supermix for probes
1
86-3010, 186-3026, 186-3027,
1
86-3028
D
roplet PCR supermix
1
86-3023, 186-3024, 186-3025
O
ne-Step RT-ddPCR supermix for probes
1
86-3021, 186-3022
Droplet generator oil Droplet generator oil for probes 186-3030, 186-3005
Control
d
dPCR buffer control kit for probes
1
86-3052
Reagents for EvaGreen detection
PCR supermix QX200
™
ddPCR
™
EvaGreen
®
supermix 186-4033, 186-4034, 186-4035,
186-4036
Droplet generator oil
Q
X200 droplet generator oil for EvaGreen dye
1
86-4005, 186-4006
Control QX200 buffer control kit for EvaGreen dye 186-4052
Consum
ables and other materials
Pipets
2
0 μl pipet for sample loading
R
ainin L-20
5
0 μl pipet for droplet transfer
R
ainin L-50, L8-50
8
-channel, 200 μl pipet for oil
R
ainin L8-200
Pipet tips
F
iltered
R
ainin GP-L10F, GP-L200F
96-well PCR plates twin.tec semi-skirted 96-well plate Eppendorf 951020362
Reagent trough
A
ny
Foil plate seals Pierceable foil plate seals 181-4040
Plate sealer
P
X1
™
PCR plate sealer 181-4000
8-cap strips
A
ny
1.3 Installation and General Operation

Connect the QX200 droplet generator to a power source using only the power cord provided. Ensure the
ground is reliably connected before plugging in the instrument

Leave 10" (5 cm) clear space behind and 5" (2.5 cm) clear to the right and left for proper ventilation

Power on the droplet generator by plugging it in. The status indicator turns solid green to indicate power is on

Open and close the instrument by pressing the button on top of the green lid
U.S. standard power cord set with grounded plug
(Type 5-15P) and C5 connector (10 A/125 V)
Power supply to 5 mm DC power jack inlet