S3™ Biosafety System Class I Instruction Manual Catalog #145-1078 #145-1021 #145-1022 #145-1029 #145-1030
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Safety and Regulatory Compliance For safe operation of the S3 biosafety system with use of the S3 or S3e cell sorter, we strongly recommend that you follow the safety specifications listed in this section and throughout the manual. Safety Warning Labels Warning labels posted on the instrument and in this manual warn you about sources of injury or harm. Refer to Table 3 to review the meaning of each safety warning label. Table 3. Meaning of safety warning labels.
Safe Use Specifications and Compliance Electrical Safety Information and Classification The S3 biosafety system conforms to international regulations encompassing the accessibility of high voltages by the user. Use all protective housings, interlocks, and shields as identified in this manual. Further information about specific electrical hazards is listed in the hardware description. AC Fuse Requirements Remove power cord before replacing fuses. Fuses are 5 x 20 mm and must be rated to 250 VAC, 2.
Chemical Hazards The S3 or S3e cell sorter inside the S3 biosafety system includes a waste container that may potentially contain hazardous chemical materials depending on the sample used. Explosive or Flammability Hazards The S3 biosafety system poses no uncommon hazard related to flammability or explosion when used in a proper manner as specified by Bio-Rad Laboratories.
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Table of Contents Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 System Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Hardware Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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1 Introduction The S3™ biosafety system is a Class I aerosol containment hood designed to work with the S3 and S3e cell sorters, which work in conjunction with S3™ ProSort™ software, providing users and the environment protection from aerosols created during the cell sorting process. The S3 biosafety system is an affordable alternative to traditional large and expensive biosafety hoods commonly offered for cell sorters.
Introduction System Components The S3 biosafety system includes the following components (Figure 1): ■■ Enclosure top assembly with vinyl side panels ■■ HDPE base floor ■■ HEPA air generator ■■ HEPA filter ■■ Enclosure frame ■■ Evacuation hose ■■ USB cord ■■ Power cord ■■ Instrument manual ■■ Certification protocol Enclosure top assembly Enclosure bottom assembly HEPA air generator Legs and lower frame fixed in place with instapack bags Fig. 1. S3 biosafety system components.
Hardware Overview Hardware Overview The S3 biosafety system comprises three components (Figures 2 and 3): ■■ ■■ ■■ EPA air generator — fan system will filter air drawn into the system and evacuate filtered H air into room or house ventilation system Enclosure — composed of polycarbonate top and vinyl side panels magnetically attached to frame Base floor — solid base made of high density polyethylene Lid Electronic Housing Assembly Blower Blower Plate Assembly Upper Filter Housing HEPA Filter Low
Introduction The front panel of the S3 biosafety system has two access doors (Figure 4). ■■ ■■ Sample access door — provides access to the loading stage touch screen lock, and sort chamber luidics access door — provides access to bulk fluidics door and allows fluidics F containers to be exchanged Sample door Fluidics door Fig. 4. Front panel access doors. All four vinyl side panels are connected to the aluminum frame using magnetic attachment.
2 Operation The S3™ biosafety system is a Class I system designed to protect the user and the environment from aerosols generated during the sorting process. The system was designed to functionally exceed the requirements of the NIH Standards for Class I biosafety and exceed the functional requirements under ISAC guidelines for cell sorting. IMPORTANT! The S3 biosafety system must remain on at all times when the S3 or S3e cell sorter is inside the system.
Operation IMPORTANT! If the airflow through the HEPA filter falls below required limits for containment, the software will alert the user and stop the sort acquisition immediately. Fig. 6. Schematic of airflow within the biosafety system. Software Control The S3 biosafety system will be in full containment if both access doors are closed. If the sample access door is open, containment is still maintained.
Software Control Fig. 8. S3 biosafety system Class I status window. Fig. 7. S3 ProSort software instrument control panel highlighting S3 biosafety system status button. If any of the following occur, the S3 or S3e cell sorter will stop sample acquisition or sort and alert the user: ■■ Airflow through HEPA filter drops below required limit ■■ Filter life reaches 0% ■■ ProSort software loses communication with the enclosure Until resolved, the software will not allow running sample or sorting.
Operation Working with the S3 and S3e Cell Sorters The S3 biosafety system was custom-designed to provide an easy access interface with the S3 and S3e cell sorters. The main interaction with the instrument can be done using the two front panel access doors: ■■ ■■ ample access door — provides access to the loading stage, touch screen lock, and S sort chamber. luidics access door — provides access to the bulk fluidics door and allows fluidics to F be exchanged.
Working with the S3 and S3e Cell Sorters Fluidics Access Door The fluidics access door is positioned for easy access to the bulk fluidics (Figure 10). Changing fluidics containers can easily be done during a “hot swap” of the fluidics or during general fluidic exchanges. To perform a hot swap of the fluidics: 1. Close the user access door and click the Swap Fluidics button on the ProSort software. 2. Open the fluidics door to access the bulk fluidics area. 3. Swap the required fluid bottles. 4.
Operation Vinyl Side Panel Operation All four sides of the S3 biosafety system can be opened to access the S3 or S3e cell sorter completely for cleaning, service, or maintenance. The panels are attached to the aluminum frame using magnets. The four panels can be opened easily using one hand. The panels can be rolled up to rest on the top of the system (Figure 12). To ensure that the front is secure there is an additional magnet in the HEPA air generator to hold the panel securely when it is opened.
3 Maintenance The S3™ biosafety system has been designed to maintain the safety of the user and the outside environment. Always follow the personal protective equipment (PPE) guidelines relevant to your laboratory’s safety procedures whenever dealing with chemicals identified below. CAUTION: Biohazard! Biosafety is of utmost importance while operating this instrument.
Maintenance Filter Replacement Replacement of the HEPA filter requires proper training and should be performed by the certifying agent. Once the HEPA filter is changed the S3 biosafety system must be re-certified to ensure proper safety. Please contact a certified biosafety service vendor when the filter life reaches 10% to schedule replacement. 1. Completely shut down the S3 or S3e system and power off. 2. Power off the biosafety system main power unit and unplug. 3. Unplug the USB cable. 4.
4 Service and Certification Whenever service is conducted on the biosafety system, be sure to completely power down both the S3™ or S3e cell sorter through the main power switch and the biosafety system. Service Bio-Rad offers service support for the biosafety system. Contact Bio-Rad’s technical and service support toll free at 1-800-4BIORAD (or 1-800-424-6723) for service needs or replacement parts.
Service and Certification Certification Procedure General Information The enclosure is a ventilated cabinet for user and environmental protection. All inflow air is exhausted through a HEPA filter without recirculation. Testing is performed to demonstrate that the unit meets recommended standards for ventilated enclosures used to house automated laboratory equipment.
Certification Procedure 3. Inject PAO in such a manner to allow for uniform particle distribution across the entire face of the HEPA filter. 4. Set the 100% concentration on the photometer by sampling in the work area or by calculating from the aerosol generator output rating using the calculated or measured exhaust CFM volume of the enclosure (section 4.1.4). 5. Acceptance: Minimum upstream concentration should be >10 µg/L. 6. Insert photometer probe in the discharge of exhaust fan or duct section.
Service and Certification S3 Biosafety System Class I Certification Form S3 Biosafety System Class I Certification Form Customer Name Phone Company Location Enclosure SN Inflow Velocity Airflow readings, FPM __________________________ ___________________________ __________________________ ___________________________ __________________________ ___________________________ __________________________ ___________________________ __________________________ ___________________________
Contaminant Testing Contaminant Testing This protocol is designed to test the aerosol contamination levels that occur under normal cell sorter operation while operating the biosafety system with only the aerosol evacuation enabled (that is, without directional air flow). This test must be performed on an S3 biosafety system that has already been installed enclosing the S3 or S3e cell sorter.
Service and Certification To start sorting the Glo Germ particles: 12. Click Start on the instrument control panel to acquire a small amount of the sample. 13. Set sorting regions and gates on plots to split the main population of Glo Germ particles. Fig. 14. Example of Glo Germ dot plot and sort gates. 14. Right click on one region and select Left Sort. 15. Repeat with a second region and select Right Sort. 16. Click the Sort Logic dropdown menu to select the collection vessel 5 ml tubes. 17.
Contaminant Testing Sample the following locations for 10 min each: ■■ Near sample station ■■ Front, below bulk fluids door ■■ On top, between nozzle and filter door ■■ Right side, below vents ■■ Left side, below vents ■■ Rear, next to the AC entry After all samples have been collected: 24. Roll down both left and right panels. 25. Roll down both the front and the rear panels. 26. Run 10% bleach through the sample line for 10 min. 27. Run deionized water through the sample line for 10 min. 28.
Contaminant Testing Record Customer Name Phone Institution Location Enclosure Serial Number Date Tested Table 6. Contaminant testing — without directional airflow, under normal sorting operation.
5 Troubleshooting Table 7 describes possible errors and causes with troubleshooting steps. Table 7. Troubleshooting table. Error Possible Causes Biosafety system icon System not configured to have does not show up in a biosafety system ProSort™ software There is no fan noise System is very quiet coming from the unit Troubleshooting Steps Contact technical support to correct the configuration of the system Stand closer to fan unit and listen for fan noise.
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Ordering Information Catalog # Description 145-1078 S3 Biosafety System Class I 145-1021 S3 Cell Sorter 488 1L2D with S3 Biosafety System Class I 145-1022 S3 Cell Sorter 488/561 2L4D with S3 Biosafety System Class I 145-1029 S3e Cell Sorter 488 1L2D with S3 Biosafety System Class I 145-1030 S3e Cell Sorter 488/561 2L4D with S3 Biosafety System Class I Instruction Manual | 23
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