Instruction Manual

Section 2: Equipment and Reagents Overview
TheTrans-Blot
®
Turbo™instrumentandreagentsaredesignedtoprovidefast,efcient,and
reproduciblewesternblotsofproteingelsinaslittleas3minutes.Eachconvenienttransferpack
containsthenecessarymaterialstoefcientlytransferasingleminigel(7.0x8.5cm)oronemidiortwo
minigels(13.5x8.5cm).Thetransferpackscontaintwobuffer-soakedionreservoirstacksalongwith
eitheraprewettednitrocelluloseorPVDFmembrane.Themembranesdonotrequireanypretreatment
beforeuse.
Thereagentsandconsumablesavailableinthetransferpacksarealsoavailableinaready-to-assemble
formataspartoftheTrans-BlotTurboRTAtransferkits.Thesekitsprovidethesameoptimizedbuffers
andconsumablesasthetransferpacks,butrequirewettingofthemembranesandltersbeforeuse.
Note:Traditionalsemi-drywesternblottingconsumablescanalsobeusedwiththesystem.See
Section3.7,page15fordetails.
Fortransfer,agelissandwichedbetweentwoionreservoirstacksinthecassette.Eachcassettecan
hold1or2miniformatgels(7.0x8.5cm)oronemidiformatgel(13.5x8.5cm).Oneorbothcassettes
canbeusedforablottingrun.Ifbothcassettesarerun,theymustusethesameprotocolandhavethe
appropriatecombinationsofgels,asshowninTable1.
Thecassettescanberunindividuallyorsimultaneouslywithindependentstarttimesusingthe
sameprotocol.Thisallowstheusertoperformmultiplesequentialrunswithoutinterruption,except
forreloadingacassette.Attheendofeachcassetterun,theionreservoirstacksarediscarded.
Membranesandgel(ifneeded)canbeusedimmediatelyfordownstreamapplicationsorstoredforlater
use.
Fig.3. Cassettebaseandlid.Reversiblelidislabeledwithlock,
unlock,andsafetyhazardsymbols.
Fig.2.Trans-BlotTurboinstrument.
Cassette bay A
(upper)
Cassette bay B
(lower)
Navigation
buttons
LCD display
Alphanumeric
keypad
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Trans-Blot
®
Turbo™ Blotting System