Instruction Manual

Section 3: Trans-Blot
®
Turbo™ System Transfer Setup
TheTurboprotocol,whencombinedwithTrans-Blottransferpacks,provideshighlyefcienttransfer
forawidevarietyofproteins.However,runconditionsmayneedtobeadjustedforaparticular
proteinofinterest.ProtocolsoptimizedfordifferentproteinandgeltypesareavailableintheBio-Rad
preprogrammedprotocolsinLISTmode.
ThesystemmayalsobeusedwithTrans-BlotTurboRTAtransferkits,whichofferthesametransfer
speedandefciencyasthetransferpacks,orsuperiorperformancetotraditionalsemi-dryblotting
consumablessuchasextra-thicklterpaperandTowbintransferbuffer(speedandefciencywillbe
similartostandardsemi-dryblottingtechniques).PleaserefertotheBio-RadProteinBlottingGuide,
bulletin2895,formoreinformationonthemethodsofelectrophoretictransfer.TheProteinBlotting
Guidecanbedownloadedfromourwebsite,www.bio-rad.com,asaPDFle;orcallTechnical
Supportat1-800-424-6723torequestbulletin2895.
3.1 Transfer Using Transfer Packs
Recommendations:
•Wearglovesatalltimesduringtheblottingprocesstopreventcontaminationofthegelsor
membranes
•Transfergelsimmediatelyafterelectrophoresiswithoutanequilibrationstep
•Thetransfermembranesandstacksofthetransferpacksareprewettedanddonotrequireany
furtherpretreatment.Openthetransferpacksimmediatelybeforeusetoavoiddryingthe
membrane
•Useonlytheappropriatecombinationsofgels,asshowninTable1
•Ifyouneedtomovethemembrane,carefullyuseattweezersorforceps
SelecttheappropriatetransferpackforyourapplicationaccordingtoTable2.Thesepacksarestable
forseveralmonthsatroomtemperature.However,toextendtheirshelflife,storethemat4ºC.The
transfermembranesandstacksofthetransferpacksareprewettedanddonotrequireanyfurtherpre-
treatment(forexample,wettingofthePVDFmembrane).
Table2.Transferpackoptions.
GelSize(cm) CatalogNumberswith0.2µm
NitrocelluloseMembrane
CatalogNumberwith0.2µm
PVDFMembrane
Foroneminiformatgel,7.0x8.5 170 -4158 170 -4156
Foronemidiformatgelortwomini
formatgels,13.5x8.5
170 -4159 170 -4157
6 TechSupport:1-800-424-6723•www.bio-rad.com
Trans-Blot
®
Turbo™ Blotting System