Instruction Manual

Mini Transfer Pack (Single Mini Gel) Setup
Thepackagingfortheminitransferpackhasthetwoionreservoirstacksintheleftandrightwellsof
thetray.Therightwellcontainsthestackfortheanode(belowthegel)andislayeredwiththetransfer
membrane(nitrocelluloseorPVDF).Theleftwellcontainsthestackforthecathode(abovethegel)
(Figure5).Usethengerwellsofthetraytoaccessthestacksandeasilyliftthemfromthetray.Thetext
aboveeachngerwellsigniesthestacklocationinthecassette.Thestackandmembraneintheright
ngerwell,labeledBottom(+)isplacedontheanodeinthecassettebase.Thestackintheleftnger
well,labeledTop(–)isplacedontopofthetransfergel,closesttothecathode.
Midi Transfer Pack (Single Midi Gel or Two Mini Gels) Setup
Thepackagingforthemiditransferpackhasatraycontainingthetwoionreservoirstacksplacedon
topofeachotherinthetray.Theionreservoirstackonthetophasatabontherightsideandisfor
theanode(belowthegel).Thisstackislayeredwiththetransfermembrane.Theionreservoirstackfor
thecathode(abovethegel)islocatedbelowthemembraneandanodestackandhasatabontheleft
side(Figure6).Usethengerwellsandtabstoaccesstheappropriatestackandliftitfromthetray.As
withtheminitrays,thetextabovethengerwellssigniesthestacklocationinthecassette.Thetop
stackandmembrane,withthetabontheright,islabeledBottom(+)andisplacedontheanodeinthe
cassettebase.Thestackbelowtheanodestack,withthetabontheleft,islabeledTop(–)andisplaced
ontopofthetransfergel,closesttothecathode.Fortransferringtwominigelssimultaneously,arrange
thegelssothatthefootofthegels(low–molecularweightside)faceeachotheronthemembrane.The
gelswillhavetobeplacedlongitudinallyonthestack(Figure11).
Fig.5.Theanodestackwiththemembraneisontheright,while
thecathodestackisontheleft.
Fig.6. Theanodestackwiththemembraneisonthetopwhilethe
cathodestackliesbeneaththeanodestackandmembrane.
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