Instruction Manual

Thetransferpacksarevacuumsealed.Checkthatthemembranedoesnotliftawaywiththefoil
packaginglid.Ifnecessary,holdtheedgeofthemembraneandpeelwhileopeningthepack.
Note: ThebaseofthetrayisPETGplasticandcanberecycledaccordingtolocalregulationsforclass1
plastics.
3.2 Placing a Transfer Pack into the Cassette
SeeFigures9and10forstep-by-stepinstructionstoplaceatransferpackintoacassette.
•Themembranesandstacksaredesignedtobeliftedfromthetrayandplacedinthecassette.Do
notinvertthestackswhenremovingthemfromthetray
•Theassembledtransferpacksshouldbereasonablycenteredandatwithinthebaseofthe
cassette(anode)
•Wedonotrecommendmovingthestacksoncetheyareplaced,asthismayintroduceairbetween
thelayers.Ifthestackmustbemovedafterplacement,useablotrollertoexpelanytrappedair
bubbles.
•Eachstackusesmultiplelayersofmaterialformaximumtransferefciency.Removingstacklayers
isnotadvised
•Refrainfromaddinganyextratransferbuffertothecassette,saturatedtransferstacksprovide
ampletransferbuffer.
Figure8showsthepropernalassemblyoftheblottingsandwichinthecassetteandisapplicabletoall
transferpacks.
•Ifthegelandionreservoirstacksarelayeredoutoforderduringassemblyoftheblottingsandwich,
carefullydisassemblethecomponentssoasnottodamagethemembrane.Reassemblethestack
andusetheblotrollertoremoveairbubblesbetweenthecomponents
Fig.7.Opencassette.a.Lidwithcathode(–)ontheunderside. b.Basewithanode(+).
b.
a.
Fig.8.Properlayeringoftheassembledtransferpack.
Top(–)cassette
electrode(cathode)
Topionreservoir
stack
Blottingmembrane
Bottomionreservoir
stack
Bottom(+)cassette
electrode(anode)
Gel
8 TechSupport:1-800-424-6723•www.bio-rad.com
Trans-Blot
®
Turbo™ Blotting System