Instruction Manual

3.4 Transfer Using the Turbo Protocol
The TurbobuttonimmediatelyaccessestheMIXEDMWprogram.Itisdesignedforefcienttransferof
awidevarietyofproteinsoverabroadrangeofmolecularweights.Thesameparameterscanalsobe
accessedintheBio-RadpreprogrammedprotocolsunderLIST>BIO-RAD>MIXEDMW.
1. TurnontheTrans-BlotTurbosystemusingtheswitchlocatedontherightsideoftheinstrument.
AfteraninitialBootscreen,thesystemwillproceedtotheHomescreen.
2. OntheHomescreenpresstheNavigationbuttonthatcorrespondswithTurbo(Figure12).
3. AfterselectingTurbo,therewillbeaselectionscreenforthenumber,size,andtypeofgeltobe
transferred.Usethecorrespondingselectionbuttontochoosetheoptionforthecombinationof
gelsintherun.Theoptionvariesthecurrentfortherun(1.3Aforasingleminiformatgel,2.5Afora
singlemidior2miniformatgels).MiniPROTEAN
®
TGX™gelscanbetransferredusingthe“1Mini
Gel”protocolorthe“1MiniPROTEAN TGX”protocol,whichtransfersasingleMiniPROTEANTGX
gelin3min.
4. PresstheNavigationbuttonthatcorrespondstoA:RUNforthecassetteintheupperbayorB:RUN
forthecassetteinthelowerbay(Figure13).Abeepwillsoundtosignalthestartofthetransfer
forthechosencassette.Ifyouarerunningbothcassettes,pressthebuttontobeginonetransfer
(eitherA:RUNorB:RUN),andthenpresstheotherbuttonatanytimeduringtheruntoimmediately
startthesecondcassette.Bothcassettesmustusethesameprotocol.
5. Theprotocolwillrunautomatically.Thescreenwilldisplaytheconditionsofthetransferandthe
progressoftherun.
Fig.12.HomeScreenoftheTrans-BlotTurbosystem.
Fig.13.TransferTurboRunscreen.
Protocolname
Transferconditions
duringrun(realtime)
Starindicatesanactiverun
Programsettings
Gelformat
Selection
buttons
Navigation
buttons
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Trans-Blot
®
Turbo™BlottingSystem