Manual

UNO Anion and Cation Exchange Columns

Introduction

UNO prepacked ion-exchange columns are designed to meet the needs of the bio-chro-

matographer for rapid and reproducible high-resolution separations of biomolecules includ-

ing proteins, peptides and polynucleotides. The availability of three column sizes (bed volumes

of 1.3, 6, and 12 ml) provides unrivaled flexibility for the economical and predictable scale

up of separation and purification protocols without sacrificing resolution due to overloading.

The UNO Separation Medium

Each column contains a “Continuous Bed” support derivatized with either strongly basic

(-N

(CH

)

) or strongly acidic -SO

sulfonic groups. The novel support produces excellent

resolution of biomolecules at high flow rates and with low back-pressures. The hydrophilic

support demonstrates extremely low non-specific binding of biomolecules accompanied by

high recovery of biological activity.

Stability of the UNO Support

The columns are stable over the pH range 2–12, allowing for easy cleaning and regener-

ation. The support is compatible with aqueous solutions of guanidine-HCl and urea.

Detergents and organic solvents such as 100% methanol, 100% ethanol, 100% isopropanol ,

and 30% ACN may also be used.

Connection to Chromatography Systems

Each UNO column is supplied with a fittings kit containing two pieces of 0.02 inch ID

(1/16 inch OD) Tefzel tubing and two 1/4 x 28 flangeless fittings. Assemble one 1/4 x 28 fit-

ting onto one end of the 0.02” tubing and insert each fitting into the top and bottom of the

column. For connection to your chromatography system, attach the appropriate fitting onto the

bare end of the Tefzel tubing.

Note: Bio-Rad Laboratories does not recommend nor warranty the use of the UNO

columns with solvent delivery systems containing stainless steel parts when used with

corrosive eluents containing e.g. halide salts. We recommend the use of inert, biocom-

patible (ceramic, PEEK, titanium) solvent delivery systems for maximum column life

and recovery of sample biological activity.

Table 1. UNO Q Column Characteristics

Q-1 Q-6 Q-12

Column bed volume (ml) 1.3 6 12

Recommended max. protein loading (mg) 20 80 160

Recommended flow-rates (ml/min) 0.5 to 5.0 0.5 to 8.0 0.5 to 10.0

Column Dimensions (mm) 7 x 35 12 x 53 15 x 68

Maximum operating pressure (psi/MPa/bar) 700/4.5/48 700/4.5/48 700/4.5/48