Manual

ing protocol as described below (steps 1–7) should be used. Always reverse the flow during

this procedure so tightly-bound substances at the top of the column are quickly removed.

During this operation do not exceed more than the following maximum flow-rates.

S-1 or Q-1 1 ml/min

S-6 or Q-6 2 ml/min

S-12 or Q-12 3 ml/min

1. Wash with 2 column volumes of deionized water. Elevated backpressures may occur

when washing with deionized water. Do not exceed 700 psi.

2. Wash with 2 column volumes of 2.0 M NaCl or KCl.

3. Wash with 1 column volume of 2.0 M NaOH followed by 3 column volumes of 0.1 M

NaCl.

4. Wash with 1 column volume of 50% acetic acid followed by 3 column volumes of 0.1 M

NaCl. Wash with 1 column volume of deionized water.

5. If lipid contamination is a problem, wash with 1 column volume of MeOH followed by

3 column volumes of 0.1 M NaCl.

6. Wash with 2 column volumes of 2.0 M NaCl or KCl or the salt containing the desired

counter-ion.

7. Change the top frit and wash with 3 column volumes of start buffer.

Fig. 1. Drawing of UNO column.

Tubing, .020 I.D. x 15 cm (1)

Tube end fitting, 1/4 x 28 (2)

End cap (3)

Shield (4)

Bed support (5)

O-ring (6)

Spacer (7)

Distribution screen (8)

Frit (9)

Glass tube (10)