Manual

Frit Removal

The top frit and screen may need to be replaced after extensive column use or if increas-

ing back-pressures are noticed. Each column is supplied with a Frit Removal Tool,

Polyethylene Frit and Distribution Screen. The screen assists in uniformly distributing the

sample over the entire column surface and also acts as a pre-filter for the frit. It should be

replaced every time the frit is changed.

Column Repair

If the top of the resin bed becomes fouled and the above hygiene steps do not restore per-

formance, then a few mm of the bed should be removed using the Bed Removal Tool. The tool

will remove 4 mm of material. For detailed instructions on removing the bed material, see

the instruction sheet included with the tool. Once fouled bed material is removed, insert a

clean frit and distribution screen. The dark brown ring on the top and bottom bed support

must be removed before reinserting the bed supports into the glass tube. This proce-

dure will compress the bed which is required to obtain proper performance. This column

repair procedure can only be performed once. If the back pressure of the column is still exces-

sive, then the purchase of a UNO Replacement Column is recommended.

Storage Conditions

Prior to long-term storage, the column should be cleaned as previously described and

then washed with 3 column volumes of 20% ethanol. This will prevent microbial growth.

Store the column in a safe place at room temperature. NEVER allow the column to freeze.

References

1. Kopaciewicz, W., Rounds, M. A., Fausnaugh, J. and Regnier, F. E., Retention Model for High-

performance Ion-Exchange Chromatography. J. Chromatography, 266, 3-21 (1983).

2. A Guide to the Properties and Uses of Detergents in Biology and Biochemistry. J. Neugebauer

(Calbiochem Biochemicals).

Product Information

Catalog

Number Product Description

720-0001 UNO Q-1 Column

720-0003 UNO Q-6 Column

720-0005 UNO Q-12 Column

720-0009 UNO Q Polishing Column

720-0011 UNO Q-1 R Column, replacement column

720-0013 UNO Q-6 R Column, replacement column

720-0015 UNO Q-12 R Column, replacement column

720-0021 UNO S-1 Column

720-0023 UNO S-6 Column

720-0025 UNO S-12 Column