xMark™ Microplate Spectrophotometer User Manual This Instrument for Laboratory Use Only
xMark System, including Microplate Manager 6 software Catalog Number 168-1150 Microplate Manager 6 software Catalog Number 168-9520 Checkmark Performance Validation Kit Catalog number 168-6940 Terasaki Plate Adapter Catalog Number 168-2000 Bio-Rad Technical Services Department Open Monday–Friday, 8:00 a.m. to 4:00 p.m., Pacific Standard Time. Phone: Fax: E-mail: (800) 424-6723, option 2, option 3 (510) 741-6576 (510) 741-5802 LSG.TechServ.US@Bio-Rad.com (U.S.) LSG.TechServ.Intl@Bio-Rad.
Warranty and Regulatory Notices Warranty Statement This warranty may vary outside of the continental United States. Contact your local Bio-Rad office for the exact terms of your warranty. Bio-Rad Laboratories warrants that the xMark system will be free from defects in material and workmanship, and will meet all performance specifications for the period of one year from the date of shipment. This warranty covers all parts and labor.
Table of Contents XMARK™ MICROPLATE SPECTROPHOTOMETER USER MANUAL ....................................................... 1 1. 1.1 1.2 1.3 1.4 2. 2.1 2.2 3. 3.1 3.2 3.3 3.4 GENERAL INFORMATION ........................................................................................................................ 1 INTRODUCTION .............................................................................................................................................. 1 XMARK SPECIFICATIONS ................
xMark Microplate Spectrophotometer User Manual 1. General Information 1.1 Introduction The xMark™, Microplate Spectrophotometer provides all the flexibility required for absorbance assay. The xMark has the capability to perform standard colormetric ELISA and kinetic readings for general cell screening with 6, 12, 24, 96, 384, and 1536-well microplate formats. The xMark uses a “filter-less” design, with a wavelength range of 200–1000 nm.
xMark Microplate Spectrophotometer User Manual 2 Stability & Drift ≤ 0.010 OD at OD=1 at 490 nm single wavelength reading Warm-up time 3 minutes Minimum kinetic read interval 96-well plate: 8 seconds single wavelength 384-well plate: 15 seconds single wavelength 1536-well plate: 35 seconds single wavelength Shaking Programmable time period: 0–999 seconds Shaking mode: Linear and Orbital Shaking speed: High, medium, low and manual speed Incubation 25°C to 45°C, programmable in 0.
xMark Microplate Spectrophotometer User Manual 1.3 External Features 2 3 1 4 5 9 6 7 8 1. Power switch 2. Open/Close button 3. Status LED (Green = idle, Orange = plate reading or working, Red = Error) 4. Door of the plate loader 5. Cooling fan 6. Power cord receptacle 7. Fuse φ 5.2 x 20, 6.3A/AC 250V, time lag type 8. Docking bolt 9. USB 2.
xMark Microplate Spectrophotometer User Manual 1.
xMark Microplate Spectrophotometer User Manual 2. Equipment Set-up and Installation 2.1 Unpacking the xMark™ Prior to opening the box, check for any outer damage. Document any damage, if present. Place the box upright with the TOP label facing up and open the box. Remove the instrument from the box in this position only. Warning! Grasp the instrument firmly under the right and left sides before attempting to lift or carry the instrument out of the box. Check the instrument for damaged or broken parts.
xMark Microplate Spectrophotometer User Manual In addition, keep a space of at least 15 cm between the rear of the instrument and the wall, to remove the power cord from the power cord receptacle.
xMark Microplate Spectrophotometer User Manual 3. Using the xMark™ The xMark is operated through the Protocol dialog boxes in Microplate Manager. See the Microplate Manager User Guide for instructions on specifying the wavelengths selection and operating the xMark through the Protocol dialogs. 3.1 Loading a Plate To load a plate into the xMark, press the Open/Close button on the front of the instrument or click on the Reader Door: Open button in the Protocol dialog box in Microplate Manager.
xMark Microplate Spectrophotometer User Manual 3.4 Incubator Controls The incubator in the xMark is controlled from within Microplate Manager. The incubator set point is set from within Microplate Manager. 4. Disinfection Procedure All parts of this instrument that can come in contact with patient sera or positive samples must be handled as hazardous items. Bio-Rad Laboratories recommends using gloves when performing maintenance or working with the instrument.
xMark Microplate Spectrophotometer User Manual 5. Product Information 168-1150 xMark Microplate Spectrophotometer System, 110/230 V, with User Manual 168-9520 MPM 6.
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