ZOE™ Fluorescent Cell Imager Instruction Manual Catalog #145-0031
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Safety Compliance This instrument has been tested and found to be in compliance with all applicable requirements of the following safety and electromagnetic standards: n EN 61010-1:2010 — Electrical Equipment for Measurement, Control, and Laboratory Use n UL Std No. 61010-1:2012 — Electrical Equipment for Measurement, Control, and Laboratory Use, Part 1: General Requirements n n n n CAN/CSA C22.2 No.
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Table of Contents Chapter 1: Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Instrument Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Graphical User Interface (GUI) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 2 2 3 Chapter 2: Setting Up the Instrument . . . . . . . . . . . . . . . . . . . . . .
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1 Introduction The ZOE Fluorescent Cell Imager is an inverted imaging system with brightfield and three fluorescent channels (emitting in blue, green, and red color), suitable for routine cell culture and imaging applications. All channels are fully integrated and optimized for most commonly used fluorescent proteins and dyes; no calibration or installation is needed. It is a stand-alone instrument and a PC is not needed to operate it.
Instrument Overview The front panel of the ZOE cell imager (Figure 1) includes: ■■ ■■ ■■ ■■ ■■ ■■ Screen — 10.
Touch screen with antiglare and antifingerprint treatment Light shield Power inlet USB port Motorized sample stage Power switch USB port Coaxial coarse/fine focus knob Fig. 1. Elements of the ZOE Fluorescent Cell Imager. Though not shown in full instrument image, both left and right sides have focus knobs. (They can be seen in inset image of the back of the instrument.) Specifications The specifications for the ZOE Fluorescent Cell Imager are shown in Table 1. Table 1.
Table 1. ZOE Fluorescent Cell Imager specifications. (cont.) FOV 0.70 mm2 Camera Monochrome, 12 bit CMOS, 5 megapixels Image format JPEG, TIFF, RAW (8 and 12-bit) Internal memory 16 GB Focus Coarse and fine, manual adjustment Stand-alone instrument Yes LCD monitor 10.1", color, LCD, touch screen with antiglare and antifingerprint treatment, 1,280 x 800 pixels image resolution, 80–180° angle tilt range Ports 2 USB ports Dimensions 33 x 32 x 30 cm (13 x 12.6 x 11.6") Weight 9 kg (19.
2 Setting Up the Instrument Menus on the touch screen can be customized. To change the default instrument settings, tap the icon located in the upper left corner of the screen (Figure 2) and select Settings. 1. In the Settings window that opens, tap the option you want to modify. 2. Make the desired modifications and tap to exit. Options on the following menus can be modified. Camera The default values for Gain, Exposure time, LED Intensity, and Contrast can be changed.
System The following information is available in this menu option: ■■ Legal information ■■ Android version ■■ Kernel version ■■ Vision board drive version ■■ Driver board firmware version ■■ Galileo software version ■■ Firmware update ■■ System test posting Firmware update To update firmware on the instrument: 1. Insert a USB flash drive with the firmware update file into USB port. The firmware file must be saved in the root directory of the USB flash drive 2.
3 Viewing Cells; Capturing and Processing Cell Images Tap the appropriate icon, located on the right side of the touch screen (Figure 2), to access the Position, Live, Gallery, or Merge functions. Position Mode The unit automatically goes into Position mode when it is powered on. The display area of the touch screen shows the top view of the sample stage. The red crosshair is always over the objective lens (Figure 2) and represents the actual imaging spot.
Live Mode The Live function is used for viewing samples illuminated in one of the four channels (brightfield or one of the three fluorescent channels) and for acquiring cell images. Motorized Stage and Field of View The ZOE Cell Imager’s motorized stage, with up to 6 mm of travel, enables rapid visualization of a large sample area. Direction and speed of the stage movement are controlled through the touch screen, and display magnification can be instantly increased using the pinch-to-zoom gesture.
Focus position indicator on the left side of the touch screen shows the Z axis position number. The number is updated during focusing (Figure 4). Knowing the approximate Z number for the tissue culture vessels (Table 2) you use can speed up focusing. Table 2. Z axis position range of tissue culture vessels.
Improving Image Contrast of Brightfield Channel A ring of green LEDs is used as a light source in the brightfield channel. Using a green light source reduces chromatic aberration and results in improved image contrast compared to white light. The Q1, Q2, Q3, and Q4 checkboxes at the top of the brightfield channel edit controls panel (Figure 5) toggle quadrants of the ring of LEDs on and off. This control can be used to achieve phase-like image contrast.
Optimizing Imaging Parameters for Fluorescence Imaging Use the Edit menu to adjust these precapture parameters (Figure 7): Gain, LED Intensity, and Contrast values; Exposure time (msec); and quadrant illumination. Drag the slider bar to adjust a parameter. Tap the – and + controls at either end of each slider bar for fine adjustments.
Image Capture To capture the image of the sample displayed in the viewing area, tap the Capture (Figure 4) icon. The captured image will remain displayed on the screen (action mode will change from Live to Gallery) and the image file will automatically be saved to the internal memory. Captured images can be edited or used in multicolor image merges. For more details go to Capturing Images for Multicolor Merge. Gallery Mode All captured images can be accessed in the Gallery mode.
Delete file File information File name Select for merge Merge selection indicator Clear all image selections Settings Access to functional modes Image folders Export to USB key Edit Pinch-to-zoom and change magnification Film strip Fig. 10. Gallery mode. Selected folder, in this case the folder with images captured using the brightfield channel, is illuminated.
Image Merge Controls For more details on creating merged images go to Capturing Images for Multicolor Merge. Select for merge — tap the checkbox icon (Figure 11) to select an image for use in creating a multicolor merge, selected images are automatically uploaded into the Merge mode. Note: Only one image per color channel can be used. To replace a selected image with a new one, tap the Select for merge control in the new image. This automatically overwrites the previous selection in the particular color.
Merge Mode In this mode previously selected single-color images are overlaid into a multicolor image (Figure 13). For more details on capturing and selecting images for merge, go to Capturing Images for Multicolor Merge. Clear all merge selections Individual color channels can be Active, Inactive, or Empty To save a merge into the Gallery Edit Fig. 13. Merge mode.
Tapping an active channel (image is displayed) hides the particular color from display, tapping it again makes it visible again. Edit — tap the color for which you wish to adjust brightness and contrast. Drag the slider bar or tap the + and – controls for fine adjustments (Figure 15). Note: Brightfield images cannot be edited in Merge mode. Any edits to a brightfield image must be made in Gallery mode and saved.
Capturing Images for Multicolor Merge Using the ZOE Fluorescent Cell Imager’s onboard software, images from up to four channels can be overlaid into a multicolor image merge. When capturing images that will be overlaid into a merge, all images should come from the same area of the sample and care should be taken not to inadvertently swipe the screen and move the stage. All images used in a merge must be of the same magnification.
A B Fig. 16. Selecting an image for merge. A, the displayed file, BPAE tubulin.jpg, file has not been selected for merge. The merge selection indicator (upper right side of screen) shows that an image from the blue folder has been selected for a merge. B, the displayed image (BPAE tubulin.jpg) file has now been selected for merge. The merge selection indicator shows that images from both the blue and the green folders have been selected and are uploaded into the merge mode.
4 Maintenance and Troubleshooting The ZOE™ Fluorescent Cell Imager requires little maintenance for proper operation. However, with long and constant use, the cell imager will need to be cleaned and some other maintenance performed. Cleaning the ZOE Cell Imager The instrument should be cleaned on a regular schedule to remove any debris or dirt that might interfere with proper function. Cleaning the Instrument Body Always turn off the instrument and disconnect the power cable before cleaning the case.
Cleaning the Objective Lens 1. Raise the objective lens to its highest focus position. 2. Unplug the instrument. 3. Put a drop of recommended lens cleaner on lens tissues or cotton swab and gently wipe the objective lens clean and dry. Recommended Lens Cleaning Fluid ■■ Lens Cleaner, Zeiss, #490133 ■■ Sparkle Optical Lens Cleaner, AmScope, #50104 Recommended Lens tissue ■■ Commercial Grade Lens Tissue, Edmund Scientific Co., #52105 ■■ Cotton-Tipped Swab Applicators, Edmund Scientific Co.
5 Software Notices and Terms Open Source Licensing Apache License, Version 2.0, January 2004 www.apache.org/licenses/ TERMS AND CONDITIONS FOR USE, REPRODUCTION, AND DISTRIBUTION 1. Definitions. “License” shall mean the terms and conditions for use, reproduction, and distribution as defined by Sections 1 through 9 of this document. “Licensor” shall mean the copyright owner or entity authorized by the copyright owner that is granting the License.
2. Grant of Copyright License. Subject to the terms and conditions of this License, each Contributor hereby grants to You a perpetual, worldwide, non-exclusive, no-charge, royalty-free, irrevocable copyright license to reproduce, prepare Derivative Works of, publicly display, publicly perform, sublicense, and distribute the Work and such Derivative Works in Source or Object form. 3. Grant of Patent License.
9. Accepting Warranty or Additional Liability. While redistributing the Work or Derivative Works thereof, You may choose to offer, and charge a fee for, acceptance of support, warranty, indemnity, or other liability obligations and/or rights consistent with this License.
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Ordering Information Catalog # Description Instrumentation 145-0031 OE Fluorescent Cell Imager, 120–240 V, includes instrument, power supply, USB key, Z instruction manual, quick guide ZOE Fluorescent Cell Imager Instruction Manual | 25
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