™ Boekel Big SHOT III Hybridization Oven Model 230402 & 230402-2 Operating Instructions N2400259 Rev.
Table of Contents Page 1. Safety ............................................................................................................. 3,4 2. Product Information........................................................................................... 4 3. Assembly.......................................................................................................... 4 3.1 Unpacking .................................................................................................. 4 3.
1. Safety The following symbols marked on the equipment mean: Caution: Read these operating instructions fully before use and pay particular attention to sections containing this symbol. Attention: Suivre attentivement les instructions avant l’usage et prêtez une attention particulière aux sections comportant ce symbole. Caution: Surfaces can become hot during use. Attention: Les surfaces peuvent devenir brûlantes pendant l’usage.
Before using any cleaning or decontamination method except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. 2. Product Information The Big SHOT III is a high capacity hybridization oven that can be utilized for all your Southern, Northern and Western blotting needs. It will hold up to ten standard 35mm diameter by 300mm long bottles or twenty 150mm long bottles.
4. Operation 4.1 Controls and Displays (see figure 1). The Power Switch: controls power to the unit and is located on the rear of the oven nearest the power cord. The Carousel Switch: stops rotation of Carousel for loading and unloading of tubes. The Display shows the chamber temperature in ˚C during normal operation. It displays the set temperature when the set button “F” is pressed.
4.2 Changing the Set Temperature 4.3 Press the “F” button once until TSEt is indicated on the display. The display will alternate between TSEt and the set temperature value. Press the “S” button to enter the “Set Temperature” mode. Adjust the Increment / Decrement Dial until the desired new Set Temperature is indicated on the display. Press the “S” button to accept and store the new value in memory.
Press the “S” button to accept the timer off condition, the unit will return to normal operating mode. At the end of the timer operation the buzzer will sound and the display will indicate “End”. The heater will shut off but the motor will continue to operate for 2 hours. To cancel the timer operation press the “F” and “S” buttons simultaneously. The unit will then resume normal operation.
key provided and slide collar to the right side of the shaft. The two “flats” can now be separated. Remove Carousel by sliding it to the right and then out. To install Carousel, reverse procedure. Warning! Sharp rotating parts. Do not attempt to remove the carousel until motor has stopped. Remove power from the equipment. Use caution when removing the carousel since it may be very hot. 4.9 Drip Tray To access the drip tray for cleaning, the tray is designed to slide out without removing the carousel.
A1902702 Nylon Mesh 230415 230423 Rocker Platform 230420 Carousels and Clips 230426 230440 230442 230210 230215 230250 Gasket for 502-0010 Bottle 15cm X 10cm, package of 5 23cm X 23cm, package of 5 Rocker Platform Microplate Carousel (holds 10 standard microplates) Large Bottle Carousel (holds 4 bottles) Multi-Tube Carousel (requires clips) Bag of Clips for 1.5/2.0mL tubes (50 pieces per package) Bag of Clips for 15mL tubes (50 pieces per package) Bag of Clips for 50mL tubes (50 pieces per package) 6.
Catastrophic Heater Condition: In the event that the chamber temperature would reach 120°C the systems heater will be turned OFF, and will on longer heat the chamber. Also the heat indicator lamp on the control panel will remain illuminated and an Alarm will sound for a short period of time. To reset the unit back to normal operation cycle the power (OFF/ON) at the units power switch on the rear of the oven.
8. Door Reversal: Figure 3 8.1 The door on the hybridization oven is reversible for visibility. To change the doors position simply remove the 3 screws & 3 caps as show in figure 3. Using the same screws reposition the door to the side desired, be sure that all screws are secured properly. Place the caps in the unused holes that the hinge previously was mounted to. 9. Maintenance and Service No routine maintenance is required. 9.
contact the “Boekel” customer service department at 1-800-336-6929, to request a copy of the calibration procedure. 9.2 Cleaning Disengage power cord prior to cleaning. The floor of the chamber is protected with a removable stainless steel drip tray. If a spill occurs, use appropriate clean up procedures as required for radiation or biohazard control. The outer casing can be cleaned with a cloth dampened with water. Do not submerge or immerse the Big SHOT in water.
10. Warranty When used in laboratory conditions and according to these operating instructions Boekel warrants this product to be free of defective material and workmanship for a period of two years from the date of manufacture. The liability of Boekel for any defective equipment during the warranty period shall be limited to the repair of such equipment or replacement thereof without charge for parts or labor. 11.
