™ Boekel Little SHOT III Hybridization Oven Model 230502 & 230502-2 Operating Instructions N2400308 Rev.
Table of Contents Page 1. Safety ................................................................................................................ 3 2. Product Information........................................................................................... 4 3. Assembly.......................................................................................................... 4 3.1 Unpacking .................................................................................................. 4 3.
1. Safety The following symbols marked on the equipment mean: Caution: Read these operating instructions fully before use and pay particular attention to sections containing this symbol. Attention: Suivre attentivement les instructions avant l’usage et prêtez une attention particulière aux sections comportant ce symbole. Caution: Surfaces can become hot during use. Attention: Les surfaces peuvent devenir brûlantes pendant l’usage.
2. Product Information The Little SHOT III is a medium capacity hybridization oven that can be utilized for all your Southern, Northern and Western blotting needs. It will hold up to 4 standard 35mm diameter by 300mm long bottles or eight 150mm long bottles. Bottles are available in four lengths – 100, 150, 225 or 300mm. The Little SHOT can also be adapted for use with 1.5/2.0mL, 15mL and 50mL conical centrifuge tubes with optional Carousel.
4. Operation 4.1 Controls and Displays (see figure 1). The Power Switch: controls power to the unit and is located on the rear of the oven nearest the power cord. The Carousel Switch: starts/stops rotation of Carousel for loading and unloading of tubes. The Display shows the chamber temperature in ˚C during normal operation. It displays the set temperature when the set button “F” is pressed.
4.2 Changing the Set Temperature 4.3 Press the “F” button once until TSEt is indicated on the display. The display will alternate between TSEt and the set temperature value. Press the “S” button to enter the “Set Temperature” mode. Adjust the Increment / Decrement Dial until the desired new Set Temperature is indicated on the display. Press the “S” button to accept and store the new value in memory.
At the end of the timer operation the buzzer will sound and the display will indicate “End”. The heater will shut off but the motor will continue to operate for 2 hours. To cancel the timer operation press the “F” and “S” buttons simultaneously. The unit will then resume normal operation. If the timer has not been switched off during the 2 hour period the motor will stop and the display will indicate “End”. 4.5 Using the High Temperature Alarm The controller is programmed with a high temperature alarm.
disengage the front mounting point. To install Carousel, reverse procedure. Warning! Sharp rotating parts. Do not attempt to remove the carousel until motor has stopped. Remove power from the equipment. Use caution when removing the carousel since it may be very hot. 4.9 Drip Tray To access the drip tray for cleaning, it is necessary to remove the carousel (see section 4.8). Use caution when removing the drip tray since it may be very hot. 4.10 Reducing the risk of a Hybridization Bottle leak 4.10.
230542 230210 230215 230250 Multi-Tube Carousel (requires clips) Bag of Clips for 1.5/2.0mL tubes (50 pieces per package) Bag of Clips for 15mL tubes (50 pieces per package) Bag of Clips for 50mL tubes (50 pieces per package) 6. Fault Diagnosis Symptom 1. Unit does not operate 2. Chamber temperature does not rise when expected 3. Temperature continues to rise when not expected 4. No rotation, Display illuminated Possible Cause a. Unit not switched on b. Unit not plugged into power supply c.
7. Technical Specifications This equipment is for indoor use and will meet its performance figures within the ambient temperature range of 0C to 35C, with maximum relative humidity of 80% noncondensing. Temperature range Stability Overall accuracy Temperature display resolution Supply ratings Power ratings Heating rate Speed rotation (Ambient +10C) to 80C. +/- 0.5C +/- 0.5C 0.1C 230502 115V±10%, 2.8A, 60Hz. 230502-2 230V±10%, 1.6A, 50/60Hz.
8.3 Replacement of Fuses Disconnect the unit from the power supply. Remove the plug from the socket in the back of the unit. Pull back on the fuse drawer catch. Pull out the fuse drawer (see Figure 4). Check and replace with the correct fuses if necessary. The fuses should be 5mm x 20mm: 230502 (115V) 4.0A/250v 3AG SLO-BLO 230502-2 (230V) 4.0A/250v 3AG SLO-BLO Push the fuse drawer back in. Reconnect unit to the power supply. OPEN FUSE HOLDER AT THIS POINT BY PULLING DOWN.
10. Service It is required to obtain a Returned Material Authorization (RMA) number before any Boekel products can be returned for any reason. A Decontamination Certificate must be completed, signed by the user, and returned to Boekel Scientific prior to receiving the RMA number. Please be sure to mark the outside of the returned goods package with this RMA number to ensure prompt handling. Boekel Scientific 855 Pennsylvania Blvd.