Operation Manual Fraction Collector C�660 096969 en
Table of contents Table of contents 1 2 3 4 5 About this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.1 Reference documents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.2 Trademarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.3 Abbreviations . . . . . . . . . . . . . . .
Table of contents 6 7 8 9 10 11 4 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 6.1 Control elements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 6.2 Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19 6.3 Operating the instrument . . . . . . . . . . . .
1 1 About this manual About this manual This manual describes the Fraction Collector C-660 and provides all information required for its safe operation and to maintain it in good working order. It is addressed in particular to laboratory personnel and operators. NOTE The symbols pertaining to safety (WARNINGS and ATTENTIONS) are explained in chapter 2. 1.
2 2 Safety Safety This chapter highlights the safety concept of the fraction collector and contains general rules of behavior and warnings from hazards concerning the use of the product. The safety of users and personnel can only be ensured if these safety instructions and the safetyrelated warnings in the individual chapters are strictly observed and followed, therefore, the manual must always be available to all persons performing the tasks described herein. 2.
2 2.4 Safety Warning notices used in this manual WARNING Generally, the triangular warning symbol indicates the possibility of personal injury or even loss of life if the instructions are not followed. WARNING Electrical hazard. WARNING Harmful to life forms. WARNING Inhalation of harmful substances. ATTENTION With the “Read this” symbol, ATTENTION indicates the possibility of equipment damage, malfunctions or incorrect process results if the instructions are not followed.
2 Safety WARNING Potential risk if solvent vapors accumulate within the instrument housing. • Beware of damaged or cracked glass parts. • Beware of dangerous, flammable or explosive samples. • Operate the instrument in a fume hood or in an adequately ventilated work area. 2.5.2 Other hazards WARNING Certain solvents within or in the vicinity of the Pump Systems can form peroxides and/or are highly inflammable.
3 3 Technical data Technical data This chapter introduces the reader to the fraction collector and its main components. It contains technical data, requirements and performance data. 3.1 Scope of delivery Check the scope of delivery according to the order number. NOTE For detailed information on the listed products, see www.buchi.com or contact your local dealer. 3.1.1 Standard instrument Table 3-1: Standard instrument 3.1.
3 Technical data Table 3-2: Standard accessories (cont.) Product Order number Regional power cord, 1.5 m: Type CH 10010 Type Schuko (D,F) 10016 Type GB 17835 Type USA 10020 Type AUS 17836 Operation Manual: 3.1.3 German 96968 English 96969 Spanish 96970 French 96971 Italian 96972 Optional accessories Table 3-3: Optional accessories Product Order number Waste valve, valve for the Fraction Collector. Prevents dripping during the fraction change.
3 Technical data Table 3-3: Optional accessories (cont.) 9 8 10 6 5 7 Product Order number e 4 racks FC 12, set of 4 racks FC 12 – each for 12 tubes at 250 mL with a diameter of 40 mm (without tubes). 44957 f 4 racks FC 30, set of 4 racks FC 30 – each for 30 tubes at 50 mL with diameter of 26 mm (without tubes). 44958 g 4 racks FC 60, Set of 4 racks FC 60 – each for 60 tubes at 20 mL with diameter of 17 mm (without tubes).
3 3.2 Technical data Materials used Table 3-4: Materials used 3.3 Component Material designation Housing PUR foam Tubing FEP Fitting POM Ferrule ETFE Drip tray Steel Chromatography table Aluminum Technical data overview Table 3-5: Technical data Dimensions ( W×D) 465×470 mm Height (H) Adjustable from 370 mm to 460 mm Weight 14 kg Connection voltage 100 – 230 VAC ± 15% Power consumption max.
3 Technical data Table 3-5: Technical data (cont.) Fuse T500 mA L 250 V Peak detection types Peak threshold Peak detection, number Monitoring of 1 or 2 detector signals Display Back-lit LCD graphic display 28×64 pixels (58×29 mm) Dialog languages en / de / fr / it / es / jp Interfaces 2× RS - 485 (system bus) 1× RS - 232 (process data output) 1× Mini-DIN (waste valve) 1× Mini-DIN (TTL In / alarm monitoring and/or ext.
4 4 Description of function Description of function This chapter explains the basic principle of the fraction collector and provides a functional description of the assemblies. The design of the fraction collector enables the automatic collection of both organic and aqueous fractions without metal contact. 4.1 Overview over the instrument a Tubing guide b FEP tubing c Collector arm d Controls e ON/OFF switch f FC 60 rack with glass tubes g Drip tray 2 4 1 3 5 6 7 Fig. 4.
5 5 Putting into operation Putting into operation This chapter describes the installation of the fraction collector and gives instructions on initial start-up. NOTE Inspect the instrument for damages during unpacking. If necessary, prepare a status report immediately to inform the postal company, railway company or transport company. Keep the original packaging for future transport. 5.
5 5.3 Installation 5.3.1 Power connection Putting into operation Connect the power cord. Fig. 5.1: Connecting the power cord 5.3.2 Fitting and ferrule 2 The tubing a containing the fraction comes from the chromatography column. The fitting b and ferrule c are attached to the free end. Mount the fitting b and ferrule c. 3 1 Press the suction tubing a and the ferrule c against a table surface to firmly seal the tubing end with the ferrule. Fig. 5.2: Installing fitting and ferrule 5.3.
5 Putting into operation The collector head e is found on the collector arm h of the fraction collector. Clamp the tubing guide f (metal pipe) tightly into the collector head g. 3 Ensure that the height of the tubing guide f is correct. It should be at least 1 cm above the tube rim, so that it will not damage the tubing during operation. 6 7 Now direct the FEP-tubing c from valve outlet b and afterwards through the tubing guide f, so that it pokes out approximately 3 mm.
6 6 Operation Operation This chapter explains the operating elements and possible operating modes. It gives instructions on how to operate the Fraction collector properly and safely. ATTENTION Check the glassware for damages prior to each operation and use only glassware in perfect condition. Glassware with cracks, stars or other damages can fail during operation. Make sure that all fittings and tubings are hand-tight. 6.
6 6.2 Operation Display Status line Mode line Navigation mode Editing mode Fig. 6.2: Overview of the display The display is divided into Status and Mode lines. The status line is fixed. On the left side it shows the five potential states of the Fraction Collector. On the right side the status line shows the remaining lead time, the remaining collection time or the residual volume of the relevant fraction.
6 Operation The mode line shows the navigation and editing mode you are in. It can be controlled with the selector switch by rotating and pressing. Parameters surrounded by lines cannot be changed. Fig. 6.3: Parameters surrounded by lines Parameters highlighted with black background can be selected and changed. The scroll bar on the right indicates your position within the menu. Fig. 6.4: Parameters highlighted with black background With the selector switch you can navigate through the menu.
6 6.3.2 Operation Manual control / manual mode Overview of the menu navigation Fig. 6.5: Overview of the menu navigation Parameters Table 6-3: Main menu Main menu Description Fraction Displays the current fraction. Time or volume It is possible to collect according to time and volume. This setting is selected in the Parameter menu under the Collect point. Detector The line appears when a detector has been selected. Parameter Jumps into the Parameter Menu.
6 Operation Table 6-4: Parameter menu Parameter menu Description back Jumps back into Main menu. Rack type The predefined rack types FC4 – FC60 and Syncore Racks R4 – R96 can be selected. Customized racks are saved under C01 - C10 and U11. Order The sequence according to which the individual fractions are filled into the tubes can be entered. T his is the best choice for the racks FC12, 30 and 60 as this order corresponds to the rack numeration.
6 Operation Table 6-4: Parameter menu (cont.) Parameter menu Description Between peaks Fractionation time or volume, if the detector signal is below threshold. Save as The entered parameters can be saved under P01 – P10. Programs Jumps into the Program menu. Rack definition Jumps into the Rack Definition menu. Options Jumps into the Options menu. Table 6-5: Program menu Program menu Description back Jumps back into the Parameter menu.
6 Rack Operation Shows the selected rack. Pos. X 1The starting point of the first tube in the X-direction is given. Pos. Y 1The starting point of the first tube in the Y-direction is given. Table 6-8: Rack menu 1 (rectangular rack) (cont.) Rack menu 1 Description Show Shows the position of the individual tubes in the rack on the display and simulates the progression of the collector arm. Save as The entered parameters can be saved under C01 – C10.
6 6.3.4 Operation Interface RS 232 The interface works with 9600 Baud, straight parity, 8 data bits and 1 stop bit. Output is provided via a PC or a printer. An example is shown in Figure 5.8. The header of the table is always written in English and appears when a program is started (transition from rest status into collecting status). Data lines are then written in an interval of one second until the program is finished or the rest status has been reached again.
6 Operation Table 6-9: Parameters for configuration Parameter Description Detector 1 or 2 A detector must be selected and connected to the correct socket. This definition determines which detector signal will be evaluated for a fraction change. If no detector has been selected, the peak collection function is turned off. The peak collection function is automatically activated at the start when a detector is selected.
6 Operation Table 6-9: Parameters for configurationm (cont.) Parameter Description Sensitivity The adjustment of various switching sensitivities. Range 4 shows the most sensitive, Range 1 the most sluggish switching behavior. This function enables adjustment of the collection function to the relevant slope of the peak. Peaks with short elution times have steep flanks and the switching decision must be correspondingly quick.
7 7 Maintenance Maintenance This chapter provides instructions on all required maintenance to keep the instrument in good working condition. WARNING All maintenance and repair work requiring the opening or removal of instrument covers and lids must be carried out by trained personnel and only with the tools provided for this purpose. WARNING Electrical hazard: • P rior to any maintenance work on the instrument switch off the power supply and remove all sources of flammable vapor.
8 8 Troubleshooting Troubleshooting The following chapter describes how to resume operation of the instrument in the event of any minor problem. It will list some possible occurrences, their probable cause and suggests how to remedy the problem. The troubleshooting table below lists possible malfunctions and errors of the instrument and describes operator enabled courses of action to correct some of tubing problems by him or herself.
8 8.2 Troubleshooting Customer service Only authorised service personnel are allowed to perform repair work on the instrument. These persons have comprehensive technical training and knowledge of possible dangers which might arise from the instrument. Contacts for official BUCHI customer service offices are given on the BUCHI website at: www.buchi. com. If malfunctions occur on your instrument or you have technical questions or application problems, please contact one of these offices.
9 9 Shutdown, storage, transport and disposal Shutdown, storage, transport and disposal This chapter instructs on how to shut down the instrument, how to pack it for storage or transport and specifies the storage and shipping conditions. 9.1 Storage and transport Switch off the instrument and remove the power cord. Disconnect all cables and tubings and remove all liquids and dusty residues before packaging the instrument.
10 10 Spare parts Spare parts This chapter lists spare parts, accessories, and optional extras, including all of the relevant order information for ordering from BUCHI. Always state the product designation and part number when ordering any spare parts. Use only genuine BUCHI consumables and spare parts for maintenance and repair to ensure optimum system performance and reliability. Prior written permission of the manufacturer should be obtained before any modifications are made to the spare parts used.
11 11 Declarations and requirements 11.1 FCC requirements (for USA and Canada) Declarations and requirements English: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to both Part 15 of the FCC Rules and the radio interference regulations of the Canadian Department of Communications. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.
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BÜCHI Labortechnik AG CH-9230 Flawil 1 / Switzerland T +41 71 394 63 63 F +41 71 394 65 65 www.buchi.
