Operation Manual UV-Vis Detector C�640 11593492 en
Table of contents Table of contents 1 2 3 4 About this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 2.1 User qualification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 2.2 Proper use . . . . . . . . . . .
Table of contents 5 6 7 8 9 4 4.1.2 Icons on the graphical display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 4.1.3 Push-buttons of the front panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 4.2 Connections at the rear panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 4.3 The cell compartment . . . . . . . . . . . . . . . . . . . . . . . .
1 About this manual 1 About this manual This manual describes the UV-VIS Detector C-640. It provides all information required for its safe operation and to maintain it in good working order. It is addressed to laboratory personnel in particular.
2 2 Safety Safety This chapter points out the safety concept of the instrument and contains general rules of behavior and warnings from direct and indirect hazards concerning the use of the product. For the users safety all safety instructions and the safety messages in the individual chapters shall strictly be observed and followed. Therefore, the manual must always be available to all persons performing the tasks described herein. 2.
2 2.4 Safety Safety warnings and safety signals used in this manual DANGER, WARNING, CAUTION and NOTICE are standardized signal words for identifying risk levels, related to personal injury and property damage. All signal words, which are related to personal injury are accompanied by the general safety sign.
2 Safety Explosive gases, explosive environment Harmful to live-forms Device damage Wear laboratory coat Wear protective goggles Wear protective gloves Additional user information Paragraphs starting with NOTE transport helpful information for working with the device / software or its supplementaries. NOTEs are not related to any kind of hazard or damage (see example below). NOTE Useful tips for the easy operation of the instrument / software.
2 2.5 Safety Product safety Safety warnings in this manual (as described in section 2.4) serve to make the user alert and to avoid hazardous situations emanating from residual dangers by giving appropriate counter measures. However, risks to users, property and the environment can arise when the instrument is damaged, used carelessly or improperly. 2.5.1 General hazards The following safety messages show hazards of general kind which may occur when handling the instrument.
2 Safety Notice Risk of instrument damage by wrong mains supply. • • 2.5.2 External mains supply must meet the voltage given on the type plate. Check for sufficient grounding. Safety measures Always wear personal protective equipment such as protective eye goggles, protective clothing, and gloves when working with the instrument. 2.5.3 Built-in safety elements and measures High voltage and electrostatic charges • Safety current limitation. • Grounded housing to arrest electrostatic charges. 2.
3 3 Technical data Technical data This chapter introduces the reader to the instrument and its specifications. It contains the scope of delivery, technical data, requirements and performance data. 3.1 Scope of application and delivery The UV-VIS Detector C-640 is available as powerful detector for the Sepacore system. It can be used in conjunction with the SepacoreControl software, connected to the C-620 control unit or without the use of the software, connected to the C-660 fraction collector.
3 3.1.2 Technical data Standard equipment Each part listed with an order number can be ordered separately as spare part. Table 3-2: Standard equipment Product Order no. UV-VIS detector unit C-640 12 Preparative flow cell for C-640 11057949 Fitting and ferrule 1/8” (2 pcs. each) 044261 Fitting and ferrule 1/16” (2 pcs.
3 Technical data FEP tube inner diameter 1/16”, outer diameter 1/8” 044354 FEP tube inner diameter 1/32”, outer diameter 1/16” 044357 Test cell for preparative cell CD containing Operating Instructions en / de / fr / it / es / cn / jp Power cord RS232 cable 3.2 Optional accessories Table 3-3: Optional accessories Product Order no. Cinch cable 045216 For connecting the C-640 directly to the C-660 fraction collector without using the SepacoreControl software. 3.
3 3.4 Technical data Technical specifications Table 3-5: Technical specifications Average power consumption max. 90 W Power supply voltage 100–240 VAC ± 10% Frequency 50 / 60 Hz Fuse 2.5 A / 250 V Dimensions (w × d × h) 135 × 418 × 185 mm Weight 6 kg Wavelength range 200–840 nm (256 elements on CCD) Typical spectral half width 10 nm Accuracy of adjustment ± 1 nm Reproducibility ± 0.5 nm Noise level at test cell ± 5 x 10-5 AU at 254 nm, TC 0.
3 3.5 Technical data Configuration of the analog cinch output a Analog Output (HI) Absorbance signal output. Voltage magnitude corresponds to the actual absorbance in the cell. The conversion factor depends on the settings. Output range is from -0.2 V to 1.2 V. The analog output is single ended. b GNA (Low) Analog ground of the output signal. The analog ground is connected to the instrument housing. Figure 3-1: Configuration of the cinch outputs (female) 3.6 Light sources 3.6.
3 3.7 Signals and parameters 3.7.1 Light intensity Technical data The light intensity of a newly installed lamp used with the test cell is set to 100% on all wavelengths. These values form the reference for later tests of the light intensity. 3.7.
3 3.7.8 Technical data Scanning Scanning over the whole detection range causes a high workload on the detector and its communication lines. To keep the workload for the instrument as low as possible the sample measurement of the four predefined wavelengths for the four analog outputs is omitted during a scan. This means during a scan the measured values on to the analog outputs will not be updated - instead the last values from the time before the scan started will be passed on till the scan is finished.
4 4 Description of function Description of function This chapter explains the basic working principle of the UV-VIS Detector C-640. It also shows how the instrument is structured and provides a general functional description of its assembly. 4.1 Front panel of the instrument The front panel of the instrument consists of nine push buttons , two LED indicators the ON / OFF switch a and the graphical display .
4 4.1.2 Description of function Icons on the graphical display Icon Meaning If this icon of a small key is visible on the upper right corner of the display, the detector is remote-controlled by the software SepacoreControl while the communication with the detector is established and the initial setup is performed. In this mode the keypad of the detector is locked. s 4.1.
4 4.3 Description of function The cell compartment The cell compartment is located on the right side of the instrument: Figure 4-3: Cell compartment of the C-640 The cell itself is accessible from the right side of the instrument by opening the shutter which at the same time functions as a quick clamping lever for holding the cell in place within the optical path. Notice Risk of instrument damage by wrong handling.
4 4.3.1 Description of function Removing the cell For a safe removal of the cell from the cell compartment proceed as follows. - While opening the shutter with one hand, prevent the cell from falling down by placing two fingers of the other hand underneath of it. - In most cases the cell will be set free as soon as the shutter has been opened completely and can be taken away from the cell compartment.
4 4.3.2 Description of function Mounting the cell - Enter the cell to the cell compartment, slide it onto the groove on the left side of the compartment and hold it on place with two fingers. - Carefully close the shutter, observing the cell to be clamped properly.
5 5 Putting into operation Putting into operation This chapter describes how the instrument has to be installed. It also gives instructions for the initial startup. NOTE Inspect the instrument for damages during unpacking. If necessary, prepare a status report immediately to inform the postal company, railway company or transportation company. Keep the original packaging for future transportation. 5.1 Installation site Put the instrument on a stable, horizontal surface.
5 5.2 Installing the Detector C-640 5.2.1 Electrical connections Putting into operation Verify that the electrical requirement of the detector, stated on the back plate of the instrument, corresponds to voltage of your local electrical network.
5 5.2.3 Putting into operation Connecting the tubing Tubes with two different diameters are contained in the standard delivery of the instrument. The wider one is 1/8” in outer diameter and 1/16” in inner diameter, the tighter one is 1/16” in outer diameter and 1/32” in inner diameter. Choose the tube with the corresponding inner diameter that fits the needs of your application best and cut it into the required number of pieces each of the appropriate length.
5 2 Putting into operation - Remove the white blanking plugs from the cell and connect the column outlet to the lower cell inlet by screwing in the bolted fitting. (Using the lower inlet for the liquid coming from the column makes the removal of air bubbles from the cell a lot easier.) - Connect the outlet tubing to the upper cell outlet . - If the detector is built into the chromatograph table, make sure to guide both tubes through the side opening of the cell compartment.
5 Controlling the detector The detector can be controlled in two ways: • in local control mode using the keypad and the graphical display of the instrument itself or • in remote control via the RS232 interface using the software SepacoreControl. The SepacoreControl offers full support for the detector and the detector can be controlled in a very convenient way. Furthermore the use of the scan functionality over the full detection range of the instrument is only provided by the SepacoreControl software.
5 Putting into operation parameters of each screen (e.g. through the different wavelengths). The value of a parameter can be increased or decreased by using the UP and DOWN buttons. If a button is held down for more than 0.5 seconds the automatic repetition will start, if it is held down for more than 3 seconds, the repetition will become faster. This feature is mainly thought for adapting the values for the wavelengths. By pressing the ENTER button a value is stored.
5 5.5.2 Putting into operation Time constant Within this menu the time constant in seconds can be set for all available channels. See also chapter 3.7.4 “Time constant / data filtering”. 5.5.3 Half width Within this menu the electronic half width of the instrument can be set. See also chapter 3.7.3 “Optical and electronical half width - spectral resolution”. 5.5.4 Analog range ratio Within this menu the absorbance range for all channels of the analog output (1V) can be set. See also chapter 3.7.
5 5.6 Getting information and scanning 5.6.1 General information Putting into operation The General info menu provides basic information about the detector. Use the buttons UP and DOWN to navigate through the different items. The following information can be retrieved from this menu: Serial number, firmware version, firmware date, lamp hours, available range of wavelength, subscription frequency, model, Software and hardware version. 5.6.
5 5.6.4 Putting into operation Errors If any kind of error occurs during operation of the detector an error description together with a corresponding error number will be displayed on the graphical display. (It might be necessary to scroll through the error description if it does not fit onto the screen all at once.) If more than one error occurs at once, the description text will only reflect the most significant failure.
6 6 Maintenance and repairs Maintenance and repairs This chapter gives instructions on maintenance work to be performed in order to keep the instrument in good and safe working condition. All maintenance and repair work requiring the opening or removal of the instrument housing must be carried out by trained personnel only! Note Use only genuine consumables and spare parts for any maintenance and repair work in order to assure warranty and continued system performance. 6.
6 6.3.1 Maintenance and repairs Cleaning the cell window A contaminated cell will result in a lowered light transmission which will increase the noise level and will make zeroing difficult. The easiest way to clean the cell is to rinse the dismantled cell with suitable solvents. Therefore the cell has be removed from the instrument. The solvent type has to be chosen according to the type of the contamination. Either organic or inorganic solvents, or diluted solutions of acids (e.g.
6 Maintenance and repairs Note To change the thickness of the liquid layer (the optical path) between the two cell windows, one or both windows can be reassembled in a different orientation. (See Figure 6-2) By default the optical path through the cell window is adapted to 0.3 mm according to schema a in figure 6-2. For samples with lower concentration the length of the optical path can be increased according to schema b and c. a) b) c) 0.1 - 0.4 mm 1.1 - 1.4 mm 2.1 - 2.
6 6.4 Maintenance and repairs Replacement of the deuterium lamp ! Warning Death or serious injuries by contact with high voltage. • • This operation may only be carried out by a qualified person! Only open the housing of the product while the instrument is switched off and unplugged. Let the lamp cool down for at least 15 minutes after switching the instrument off.
6 Maintenance and repairs - Loosen the screw on the lamp holder just so far that the lamp holder becomes turnable. - Turn the bottom of the lamp holder slightly in your direction (see next picture) so that the two screws holding the lamp become accessible. 1 1 - Unscrew the two screws and at the bottom of the lamp holder and take them away.
7 7 Troubleshooting Problem Caused by / Conditions After switching the unit on no LED on Failure of the power line fuse. the front panel is lit. Electronics failure. Increased noise and drift. 7.1 Troubleshooting Solution Check the fuse. (The fuse is integrated in the main power connector on the backside of the instrument.) Call service center. Leaking cell, dirty cell, bubbles, Perform Cell test with lamp test non-transparent mobile phase, old or included. bad lamp. Check cell connections.
7 7.2 Troubleshooting Hardware error codes All errors related to a faulty hardware part of the detector are considered as hardware errors. Hardware errors are mainly detected based on physical values that are out of the working range of the instrument. In case an error code is displayed and the problem cannot be solved by switching the instrument off and on again, please note down the 8 digit error code ending with “h” and contact the service center.
8 8 Shutdown, storage, transport and disposal Shutdown, storage, transport and disposal This chapter instructs how to shut down and to pack the instrument for storage or transport. Specifications for storage and shipping conditions can also be found listed here. 8.1 Storage and transport Switch off the instrument and remove the power cord. Disconnect all cables and tubings and remove all liquids and dusty residues before packaging the instrument.
9 9 Declarations and requirements 9.1 FCC requirements (for USA and Canada) Declarations and requirements English: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to both Part 15 of the FCC Rules and the radio interference regulations of the Canadian Department of Communications. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.
8 41 Shutdown, storage, transport and disposal C-640 Operation Manual, Version A
9 42 Declarations and requirements C-640 Operation Manual, Version A
BÜCHI Labortechnik AG CH-9230 Flawil 1 / Switzerland T +41 71 394 63 63 F +41 71 394 65 65 www.buchi.
