Large Format Cooled Gel System Vertical Slab Gel System DCX-900C INSTRUCTION MANUAL
T A B L E O F C O N T E N T S Important User Information. . . . . . . . . . . . . . . . . . . . . . . . 3-4 Section 1: General Information 1.1 Introduction and Features . . . . . . . . . . . . . . . . . . . . . . . 5 1.2 Specifications and Constructions. . . . . . . . . . . . . . . . . . . . . 5 1.3. Comb Specifications for Hand Cast Gels . . . . . . . . . . . . . . . .. . 6 6 1.4 Safety. . . . . . . .
ENGLISH IMPORTANT USER INFORMATION This Instruction Manual will explain how to use this product safely and effectively. Please read and carefully follow the instruction manual in its entirety. The triangle/exclamation mark symbol alerts the user of the product to important operational, maintenance, and/or warranty requirements. The triangle/lighting bolt symbol alerts the user of the product to potentially hazardous electrical exposure.
FRANÇAIS INFORMATION IMPORTANTE À L’USAGE DES UTILISATEURS ESPAÑOL Le présent manuel d’utilisation explique la manière de se servir efficacement du produit en conditions de sécurité. Il est recommandé de soigneusement lire la totalité du manuel, avec ses consignes et ses instructions. El presente instructivo explica la manera de usar este producto en forma segura y efectiva. Sírvase leerlo en su totalidad y seguir detenidamente las indicaciones que contiene.
SECTION 1 General Information 1.1 Introduction and Features C.B.S. Scientific offers the Large Format Cooled Vertical is a temperature controlled large capacity system for running SDS PAGE and 2-D analysis. The increased gel sizze of 20cm(w) x 22cm(l) allows a larger sample volumes, increased number of wells and longer migration distance. Table 1: Features of DCX-900C 1.
1.3 Comb Specifications for Hand Cast Gels COMB OPTIONS material: PTFE, overall length: 15.87cm 1.4 Cat. # # of Teeth Tooth Depth (mm) Thickness of Teeth (mm) Width of Teeth (mm) Recommended Max. Sample Vol.
SECTION 2 Description of Parts 2.1 Unpacking and Components Please verify that your unit comes complete with the following components: Large Format Cooled Vertical Electrophoresis System: • Lower Reservoir with double-sided cooling labyrinth, tubing adapters and stirring corral • Safety cover • Power leads (must be attached before using. See section 3.1.
SECTION 3 Instructions for Slab Gel Electrophoresis See Section 4 for casting gels using the Gel Wrap method. You may also use the 4-place Multi-Caster DCX9-MC-4. 3.1 Preparing the Electrophoresis Unit 1. Power leads are shipped separately and must be attached before using your Large Format Cooled Vertical. Thread the power lead ends into the lid receptacles and rotate in clockwise direction to hand tighten. 2. Place unit in authorized work area.
5. Slide gel plate set(s) into the core assembly with the notched plate facing in towards the upper buffer reservoir as shown in figure 4. 3 6. If running one gel, slide white plastic conversion plate into the side without the gel. 4 7. Close doors and relatch by pressing down on the white latches so that the assembly looks like that shown in figure 6. 5 9 www.cbsscientific.
3.2 Running the Gel 1. Place stirring bar into bottom of reservoir in stirring corral (as shown in figure 6). Place core assembly into lower reservoir. The anode (red) and cathode (black) electrodes are color-coded on both the core/cassette assembly and lower reservoir. Ensure the red dot on the cassette assembly is on the same side as the red receptacle on the lower reservoir. Fill core upper reservoir with freshly prepared buffer (~ 190mls).
3.3 Removing the Gel 1. Turn the power supply off and disconnect the leads from the power supply. Remove the safety cover from the unit, by placing thumbs on white posts next to red & black connectors, then pushing down while pulling up with fingers under lid as shown in figure 8. Do not remove safety cover by pulling up on leads! 2. Pull up on gel door latches, and open gel door. Remove gel sandwich from Cassette Assembly. Stain and fix according to your preferred method. 11 8 www.cbsscientific.
3.4 Gel Casting Using Gel Wrap™ Gasket Casting Method 1. Place all components in an authorized work area. You will need: glass plate set, Gel Wrap™ Gasket, spacer set, comb, 3 GPC-0002 clamps, and polyacrylamide solution. Prepare and clean glass plates by hand washing both plates with a high quality lab detergent followed by a complete rinsing with dH2O. Air-dry or use a lint-free tissue. Spray/wipe the chosen inner surfaces of the plate set with 95% ethanol and dry with lint-free tissue. 2.
5. Place the notched plate on top of the bottom assembly, starting from the bottom edge and gently easing the plate down. Verify the gasket is smooth around the edges and then clamp along the bottom. 6. Lift the assembly and stand it on the base of the clamps. For leveling, push glass plate assembly down until it stops against clamp body. Clamp the sides of the assembly with additional casting clamps on either side. As each clamp is attached, be sure the gasket is aligned between the plates forming a seal.
SECTION 4 Running Conditions 4.1 4.1.1 Recommended Power for Slab Gels: Precise electrophoresis conditions will vary according to the number and type of gels used, buffer conditions employed, power input, and the general goal of the experiment. Refer to reference section 4.2 for in depth discussions on practical and theoretical approaches to protein gel electrophoresis. SDS PAGE Gels Run Voltage Starting Current Ending Voltage Approx. Run TIme 90VDC 30mA/gel CC 110 volts/gel 4-5 hours 4.
SECTION 5 Maintenance of Equipment 5.1 Care and Handling The plastic components of the Large Format Cooled Vertical Electrophoresis Systems are fabricated from acrylic and polycarbonate. Electrodes and connectors are made from pure platinum, stainless steel, and chrome plated brass. As with any laboratory instrument, adequate care ensures consistent and reliable performance. After each use, rinse buffer chamber, gel tray and combs with de-ionized water.
SECTION 6 Ordering Information Large Format Cooled Vertical Ordering Information COMBS: See page 6. CAT. # DESCRIPTION COOLED LARGE FORMAT GEL SYSTEM- 20CM(W)* DCX-900C Cooled Large Format Gel System, CE, Complete System, glass plate dimensions 20cm(w) x 22cm(l) Accessories and Related Products for Cooled Large Format Gel System SGP20-020R Gel Wrap® Glass Plate Set SGP20-020R-DN Gel Wrap® Glass plate Set with Borosilicate back plate VGE20-0720 Gel Wrap® Gasket, 0.
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DCX-900C Large Format Instructions 9-30-13
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CONTACT INFORMATION Online Ordering www.cbsscientific.com Telephone Local and International: 858-755-4959 Toll Free: 800-243-4959 Sales E-mail Address sales@cbssci.com Technical Service E-mail Address technicalservice@cbssci.com Mailing Address P.O.
