Owner manual

DCX-900C Large Format Instructions 9-30-13

SECTION 4

Running Conditions

4.1 Recommended Power for Slab Gels:

Precise electrophoresis conditions will vary according to the number and type of

gels used, buffer conditions employed, power input, and the general goal of the

experiment. Refer to reference section 4.2 for in depth discussions on practical and

theoretical approaches to protein gel electrophoresis.

4.1.1 SDS PAGE Gels

Run Voltage Starting Current Ending Voltage Approx. Run TIme

90VDC 30mA/gel CC 110 volts/gel 4-5 hours

4.1.2 General Recommendations

If running only one gel, keep the volts the same but reduce the mA’s by half. Keep in

mind that as the thickness of gel increases, the mA’s increase proportionally.

At constant voltage, the proteins will migrate at a constant rate during electropho-

resis with adequate heating appropriate for denaturing gels. Increasing the voltage/

mA (for a single gel thickness and percentage) will speed mobility but increase the

risk of overheating.

If using cooling base the power input and the migration rate can be increased. The

joule heating generated by the higher power is offset by the cooling effect of the

buffer between the gels.

4.2 References

1. Hames, B.D. (ed.) (1998). Gel Electrophoresis of Proteins. A Practical

Approach. 3

edn. Oxford University Press, Oxford. Ch. 1,3.

2. Sambrook, J., Fritsch, Russell, D. (2001). Molecular Cloning. A Laboratory

Manual. 3

edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor,

New York. A8.40-A8.55

3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith,

J.A., Struhl, K. (ed) (1993). Current Protocols in Molecular Biology. Vol. 2,

Greene Publishing Associates, Inc. and John Wiley & Sons, Inc., Ch. 10.