Owner's manual
2
EBU-204 Mini-Blotting System Instructions 6-2-14
TABLE OF CONTENTS
Important User Information . . . . . . . . . . . . . . . . . . . . . . . .3-4
Section 1: General Information
1.1 Introduction and Features . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Speciî‚¿cations and Constructions
. . . . . . . . . . . . . . . . . . . . 5
1.3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Section 2: Description of Parts
2.1 Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.1 Components/Assembly . . . . . . . . . . . . . . . . . . . . . . . 7
Section 3: Instructions for Use
3.1 Blotting Unit Preparation . . . . . . . . . . . . . . . . . . . . . . 8
3.2 Preparing the Gel for Transfer . . . . . . . . . . . . . . . . . . . . .8-9
3.3 Standard Electro-Blot Transfer . . . . . . . . . . . . . . . . . . . . 10
3.4 Removing the Cassettes . . . . . . . . . . . . . . . . . . . . . . . 10
Section 4: Running Conditions
4.1 Recommended Buffers, Membranes & Running Conditions . . . . . . . 11
4.2 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Section 5: Maintenance of Equipment
5.1 Care and Handling . . . . . . . . . . . . . . . . . . . . . . . . 13
5.2 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Section 6: Ordering Information
6.1 Ordering Information for Mini-Blotting System . . . . . . . . . . . . . . 15