INSTRUCTION MANUAL Electrophoretic Mini-Blotting Systems Double and Triple-Wide Cat. # EBU-302 Cat. # EBU-362 Cat. # EBU-402 www.cbsscientific.
TABLE OF CONTENTS Page Important User Information 3-4 Section 1 1.1 1.2 1.3 General Information Introduction Specifications Safety 5 5 5 Section 2 2.1 2.2 Description of parts Unpacking Components/Assembly 6 6 Section 3 3.1 3.2 3.3 3.4 Instructions for Use Blotting Unit Preparation Preparing the Gel for Transfer Standard Electro-Blot Transfer Removing the Cassettes 7 7-8 9 9 Section 4 4.1 Running Conditions Recommended Buffers & Running Conditions References 10 10 Section 5 5.1 5.
ENGLISH IMPORTANT USER INFORMATION This Instruction Manual will explain how to use this product safely and effectively. Please read and carefully follow the instruction manual in its entirety. The triangle/exclamation mark symbol alerts the user of the product to important operational, maintenance, and/or warranty requirements. The triangle/lightning bolt symbol alerts the user of the product to potentially hazardous electrical exposure.
FRANÇAIS INFORMATION IMPORTANTE À L'USAGE DES UTILISATEURS ESPAÑOL Le présent manuel d'utilisation explique la manière de se servir efficacement du produit en conditions de sécurité. Il est recommandé de soigneusement lire la totalité du manuel, avec ses consignes et ses instructions. El presente instructivo explica la manera de usar este producto en forma segura y efectiva. Sírvase leerlo en su totalidad y seguir detenidamente las indicaciones que contiene.
SECTION 1 General Information 1.1 Introduction Electroblotting is a fast, easy, and efficient technique for transferring proteins from PAGE gels nitrocellulose and nucleic acids from agarose/PAGE gels onto nylon transfer membranes. The Wide Mini-Blotting Systems can effectively complete transfer in less than 2 hours compared to 6 to 24 for the more traditional “capillary” transfer. The C.B.S.
SECTION 2 Description of Parts 2.1 Unpacking the EBU-302, EBU-402 or EBU-362 Please verify that your unit comes complete with the following components: Blotting chamber • Blotting cassette with sponge pads • Safety cover with attached power leads • 2 electrode panels • Tubing adapters for cooling base 2.2 Components/Assembly www.cbsscientific.
SECTION 3 Instructions for Use 3.1 Blotting Unit Preparation 1. 2. Place the blotting chamber on a level work surface in an authorized work area. Consult applications table 4.1 for type of transfer, buffer system, membrane type and power settings.
. Place second saturated Sponge pad (referenced below as Scotch Brite) over sandwich assembly and close the cassette. Cassette Assembly 9. Remove entire assembly from pyrex dish and load into desired position (slot) in buffer chamber with black screen facing towards black terminal (cathode) and red screen facing towards red terminal (anode). www.cbsscientific.
3.3 Standard Electro-Blot Transfer 1. Fill the chamber with transfer buffer. (Buffers should be prepared fresh with reagent grade chemicals and pre-cooled). The transfer buffer should be pre-cooled and added to the top of the platinum labyrinth. The buffer should not come in contact with the banana plugs when the gel cassette sandwich is immersed in the unit. 2. Align safety cover over the unit and carefully attach. Begin cycling of coolant. 3.
SECTION 4 Applications & Running Conditions for Tank Type Electro-Blotting 4.1 Recommended Buffers, Power Settings, and Transfer Times Precise transfer conditions will vary according to the number and type of gels used, buffer conditions employed, power input, and the general goal of the experiment. Refer to the reference section for in depth discussions on practical and theoretical approaches to gel transfers.
SECTION 5 Maintenance of Equipment 5.1 Care and Handling The plastic components of the Electrophoretic Blotting units are fabricated from acrylic and polycarbonate. Electrodes and connectors are made from pure platinum, stainless steel, and chrome plated brass. As with any laboratory instrument, adequate care ensures consistent and reliable performance. After each use, rinse buffer chamber, gel tray and combs with de-ionized water. Wipe dry with a soft cloth or paper towel, or allow to air dry.
SECTION 6 Equipment and Accessories Cat. # Item EBU-402 Double-Wide Mini-Blotting System. Gel Size: 9cm x 20cm(w). Includes chamber with 2 platinum wire electrode panels, 1 color-coded gel cassette, safety cover with power leads. Additional Double-Wide Gel Cassette, color coded Double-Wide Sponge Pads, pkg. of 2 EBC-402 EBS-402 EBU-302 EBC-302 EBS-302 EBU-362 EBC-362 EBS-362 Triple-Wide Mini-Blotting System, 10cm(h). Gel Size: 10cm(h) x 33 cm (w).
