INSTRUCTION MANUAL ELECTROPHORETIC BLOTTING SYSTEM EBX-700
T A B L E O F C O N T E N T S Important User Information . . . . . . . . . . . . . . . . . . . . 3-4 Section 1: General Information 1.1 Introduction and Features . . . . . . . . . . . . . . . . . . 1.2 Specifications . . . . . . . . . . . . . . . . . . . . . . 1.3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . 5 5 6 Section 2: Description of Parts 2.1 Unpacking and Components 7 . . . . . . . . . . . . . . . . Section 3: Instructions for Use 3.1 Blotting Unit Preparation . . . . . . .
IMPORTANT USER INFORMATION This Instruction Manual will explain how to use this product safely and effectively. Please read and carefully follow the instruction manual in its entirety. The triangle/exclamation mark symbol alerts the user of the product to important operational, maintenance, and/or warranty requirements. The triangle/lightning bolt symbol alerts the user of the product to potentially hazardous electrical exposure.
FRANÇAIS INFORMATION IMPORTANTE À L’USAGE DES UTILISATEURS ESPAÑOL Le présent manuel d’utilisation explique la manière de se servir efficacement du produit en condition de sécurité. Il est recommandé de soigneusement lire la totalité du manuel, avec ses consignes et ses instructions. El presente instructivo explica la manera de usar este producto en forma segura y efectiva. Sírvase leerlo en su totalidad y seguir detenidamente las indicaciones que contiene.
SECTION 1 General Information 1.1 Introduction C.B.S. Scientific now offers the EBX-700 4-Place Blotting System for performing electro-blotting. This unit provides the capability of blotting four gels simultaneously under identical temperature controlled buffer conditions. Units include 4 freezer blocks, and 4 blotting cassettes with 4 sponge pads. Table 1: Features of EBX-700 Electrophoretic Blotting System 1.
1.3 Safety Power to the EBX-700 Electrophoresis System is to be supplied by an external DC voltage power supply that must be ground isolated so that the DC voltage output floats with respect to ground.
SECTION 2 Description of Parts 2.
Instructions for Use 3.1 Blotting Unit Preparation 1. Place 4 freezer blocks in freezer and allow enough time to freeze. 2. Place the blotting chamber on a level work surface in an authorized work area. 3. Consult applications table 4.1 for buffer system and power settings. 4. Place, do NOT drop, a magnetic spin bar (not supplied by C.B.S.) in the bottom of the tank. This maintains pH, buffer and heat circulation during the electroblot. 5. Place tank on top of the magnetic stirrer. 6.
5. Assemble blotting stack as shown below. With cassette wide open assemble components on black side in the following order: buffer saturated sponge pad, gel equilibrated in transfer buffer, buffer saturated transfer membrane, then buffer saturated blotting paper. Smooth with gloved finger or roll with glass rod to be sure no bubbles exist between the gel and the transfer membrane. blotting paper membrane gel red side foam pad black side 6. Insert blotting cassettes into the lower reservoir.
3.4 Standard Electro-Blot Transfer 1. Fill the chamber with cold transfer buffer. (Buffers should be prepared fresh with reagent grade chemicals and pre-cooled). The transfer buffer should come up to the top of the platinum labyrinth. The buffer should not come in contact with the banana plugs when the gel cassette sandwich(es) are immersed in the unit. 2. Align safety cover over the unit and carefully attach. 3.
SECTION 4 Applications & Running Conditions for EBX-700 Electro-Blotting 4.1 Recommended Buffers, Power Settings and Transfer Times 1. Using 2 freezer blocks and chilled buffer, start run at 60Volts(c) @ 370mA for 1-3 hours. Run time will vary depending on thickness of gel and sample size. 2. Buffers: ClearPAGE Transfer Buffer (cat.
Section 5 Maintenance of Equipment 5.1 Care and Handling The plastic components of the EBX-700 Electrophoretic Blotting System are fabricated from polycarbonate. Electrodes and connectors are made from pure platinum, stainless steel, and nickel plated brass. As with any laboratory instrument, adequate care ensures consistent and reliable performance. After each use, rinse all parts with de-ionized water. Wipe dry with a soft cloth or paper towel, or allow to air dry.
6.1 Ordering Information Part number Description EBX-700 4-Place Blotter, CE. Kit includes: lower reservoir, safety cover with attached leads, 4 blotting cassettes, 4 sponge pads, 4 freezer blocks, anode, cathode, and instruction manual Accessories EBX-BC-700 Accessory Blotting Cassette, includes sponge pad EBX-SP-700 Accessory Sponge Pads (set of 4) EBX-FB-700 Accessory Freezer Block HP19001 ClearPAGE Blot Sandwich NC 90x85mm 2/pk. 0.
6.2 Ordering Information for Related Products Part number Description DCX-700 Dual Cool System, CE. Fits precast gels or glass plate dimensions of 10x10 or 10x9cm(h).
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