INSTRUCTION MANUAL Horizontal/Submarine Productivity Packages High-Throughput Horizontal Productivity Packages GCSGU-014T-02 GCSGU-020T-02 GCSGU-030T-02 GCSGU-040T-02 GCSGU-2614T-02 GCSGU-2626T-02 GCSGU-2640T-02
TABLE OF CONTENTS Page Important User Information 3-4 Section 1 1.1 1.2 1.3 General Information Introduction Specifications Safety 5 5 5 Section 2 2.1 2.2 Description of parts Unpacking Components/Assembly 6 6 Section 3 3.1 3.2 3.3 3.4 3.5 Instructions for Use Submarine/Horizontal Unit Preparation Gel Casting Running the Gel Removing the Gel Comb Volumes 7 7-8 9 9-10 10 Section 4 4.1 4.2 4.3 4.
ENGLISH IMPORTANT USER INFORMATION This Instruction Manual will explain how to use this product safely and effectively. Please read and carefully follow the instruction manual in its entirety. The triangle/exclamation mark symbol alerts the user of the product to important operational, maintenance, and/or warranty requirements. The triangle/lighting bolt symbol alerts the user of the product to potentially hazardous electrical exposure.
FRANÇAIS INFORMATION IMPORTANTE À L'USAGE DES UTILISATEURS ESPAÑOL Le présent manuel d'utilisation explique la manière de se servir efficacement du produit en conditions de sécurité. Il est recommandé de soigneusement lire la totalité du manuel, avec ses consignes et ses instructions. El presente instructivo explica la manera de usar este producto en forma segura y efectiva. Sírvase leerlo en su totalidad y seguir detenidamente las indicaciones que contiene.
SECTION 1 General Information 1.1 Introduction The Submarine/Horizontal Gel units offer gel sizes (14 x 20cm) GCSGU-014T-02, (20 x 20cm) GCSGU-020T-02, (20 x 30cm) GCSGU-030T-02, (20 x 40cm) GCSGU-040T-02, (26 x 14cm) GCSGU-2614T-02 (26 x 26cm) GCSGU-2626T-02, and GCSGU-2640T-02 (26cm x 40cm). In the Submarine/Horizontal gel units, the unique tapered baffle system allows immediate gel casting directly onto the removable UV transparent gel tray without having to seal with agar or tape.
SECTION 2 Description of Parts 2.
SECTION 3 Instructions for Use 3.1 Submarine/Horizontal Unit Preparation Place Submarine/Horizontal unit on a level work surface in an authorized work area. Level the unit by placing the bubble level in the center of the unit and adjusting the four leveling screws on the of base until the unit is level. Place the adjustable height comb(s) assembly in the gel tray. Loosen the white screws holding the comb to the backing and adjust comb depth (generally 2mm).
3.2 Gel Casting The Submarine/Horizontal gel unit provides the user with two options for gel casting: (a) gel casting directly into unit using gel tray or (b) gel casting in gel tray outside of unit. A. Casting Directly into Unit Using Gel Tray. 1. Place gel tray onto raised bed of unit. Insert the tapered baffles simultaneously on either side of gel tray and slide them into the grooves next to the bed, handles to the outside. The tapered baffles will rest friction tight against the gel tray. 2.
B. Gel Casting in External Gel Caster. 1. Level external gel caster with leveling screws on base. Push one of the open sides of gel tray against black gasket. 2. Push down on gel tray until flat against bottom of tray and so both sides friction fit against the black gaskets. 3. Place gel comb(s) into desired slot(s) in gel tray and pour in hot agaorse. When agarose has cooled place gel tray into Hoizontal Unit. Remember, current travels from negative (black cathode) towards positive (red anode). 3.
2. Align safety cover over the unit and carefully attach, so not to disturb samples. Begin cycling of coolant and buffer (if used). 3. Connect the leads to the power supply, matching the color-coded red to red and black to black. See Section 4.1 for recommended power conditions. Begin separation by electrophoresis. 3.4 Removing the Gel 1. Turn the power supply off and disconnect the leads from the power supply. 2.
3.5 Maximum Well/Comb Volumes NOTE: To calculate sample well volume expressed in millimeters (mm) of height, divide maximum volume by tooth depth. TABLE 1---Combs for 14cm wide unit SGC14 Combs for 14cm wide unit Tooth Depth mm Spacing between teeth mm Overall length of comb mm Tooth width/ mm 120 1.0mm thickness volume per tooth microliters (ul) 1524 1.5mm thickness volume per tooth microliter s (ul) 2286 2.0mm thickness volume per tooth microliters (ul) 3048 3.
SECTION 4 Running Conditions 4.1 Recommended Power As a rule, optimal resolution of larger molecules is achieved during longer runs at lower voltages, whereas smaller molecules require shorter runs at higher voltages. Applied voltage gradients can therefore be anywhere in the range of 1-10 VDC/cm of gel. Using the standard buffer systems listed below, most runs will use 5 VDC/cm as a general rule. The usual run time will vary for the voltage chosen but should range from 15 to 60 minutes.
4.4 References 1. Hames, B.D., Rickwoood, D. (ed.) (1990). Gel Electrophoresis of Nucleic Acids. nd A Practical Approach. 2 edn. IRL Press, Oxford. Ch. 2. 2. Sambrook, J., Fritsch, E.F., Maniatis, T. (1989). Molecular Cloning. A Laboratory nd Manual. 2 edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. Ch 6. 3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K. (ed) (1993). Current Protocols in Molecular Biology. Vol.
SECTION 6 Submarine/Horizontal Gel Kit and Accessories Cat.# GCSGU-014T-02 GCSGU-020T-02 GCSGU-030T-02 GCSGU-040T-02 GCSGU-2614T-02 GCSGU-2626T-02 GCSGU-2640T-02 Item Submarine/Horizontal Gel Productivity Package-CE. Gel bed dimensions are 14cm x 20cm. Includes 2 gel tray and 2 combs (please specify comb cat. #’s) Submarine/Horizontal Gel Productivity Package-CE. Gel bed dimensions are 20cm x 20cm. Includes 2 gel tray and 2 combs (please specify comb cat. #’s) Submarine/Horizontal Gel Productivity Package.
Accessories-continued: HIGH-THROUGHPUT SUBMARINE COMBS, with ADJUSTABLE BACKING FOR 26CM WIDE UNITS (Available in Polycarbonate or Teflon-coated Aluminum**) Cat. # Comb dimensions Cat.# Comb Dimensions SGC26-7525-1MA 0.
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