Owner's manual

C.B.S.  Scientific 5 Adjustable Height Sequencer
SECTION 1
General Information
1.1 Introduction
The C.B.S. Scientific Adjustable Nucleic Acid Sequencers features the flexibility of running path
lengths from 22cm up to 1 meter. This adjustable feature allows for rapidly changing research
priorities for the individual laboratory researcher. More recent state-of-the-art techniques have
been adapted for use on our adjustable units including large scale screening for Gel Mobility
Shifts (gel retardation-MSA), mRNA Differential Display using RT-PCR, DNA footprinting, RNase
protection assays, as well as the classical methods of nucleic acid sequencing either by Sanger-
Coulson (dideoxy method) or the original Maxam-Gilbert reaction procedure. The units are
available in either 20cm or 33cm wide formats.
1.2 Specifications
Constructions:
Upper buffer chamber Acrylic
Lower buffer chamber/base Acrylic
Safety covers Acrylic
Electrodes Platinum wire .012" diameter
DC Power leads FR Urethane rated 7500VDC
200mA, 65ºC
Guide rods Delrin
Heat dispersion plate Anodized aluminum
Glass plates Soda-lime float glass or Low fluorescent
glass
Combs Polycarbonate, Mylar or White
Spacers Polycarbonate, Mylar or White
White spring clamps Polypropylene
Overall size
Footprint
Base size-20cm unit 30.5cm (l) x 30.5cm (w)
Base size-33cm unit 40.6cm (l) x 30.5cm (w)
Height 42, 52, 62, 82, 102cm
Recommended buffer volumes
20cm wide units
upper reservoir 700ml
lower reservoir 700ml
33cm wide units
upper reservoir 33cm 1400-1600ml
lower reservoir 33cm 1000-1600ml