CAROLINA ® Teamed with Teachers Instructions for Bottled Media
©1998 Carolina Biological Supply Company Printed in USA
Caution: Do not attempt to melt bottled media in the microwave. It is not possible to vent the bottle without contaminating the media. Heating the bottle in the microwave may cause pressure buildup, thereby creating an explosion hazard. For your safety, please follow the recommended procedures below. Preparing the Medium 1. Fill a small pot halfway with water. (Or, use a large pot for several bottles.) 2. Place the bottle(s) of medium unopened inside the pot. Loosen the cap slightly.
Pouring the Plates 1. Place the agar plates on the prepared work surface. 2. Remove the cap of the bottle you are going to use. Sterilize the mouth of the medium bottle by passing it through the flame of a bunsen burner or by wiping with an alcohol prep. 3. Lift the cover of a plate just enough to pour in the medium. 4. Pour a small amount of agar medium into the plate. Use just enough to cover the bottom of the dish. Each bottle will pour approximately five 100-mm plates.
or a thoroughly flamed wire loop. Note: You must use a new, STERILE swab or a flame-sterilized loop for each plate. 3. Rub the swab or loop over or place it in the material to be cultured. 4. Lift the lid of an agar plate. 5. Streak the plate. Use either a simple zigzag streak (Figure 1) or an isolation streaking method (Figure 2). Quickly replace the lid after making each streak. Remember: For isolation streaking, a wire loop must be used. Figure 1.
. Incubate the plates. Notes: For room-temperature incubation, place the plates agar-side up. If using an incubator, invert the plates and incubate them at 30–37° C. The specific time and temperature required for incubation depends on the medium selected. 7. Sterilize wire loops or dispose of used swabs. See Disposal section. Storage Unopened medium bottles may be stored under refrigeration for up to 1 year or at room temperature for up to 6 months.
Ordering Information For petri dishes we recommend: 74-1350 Sterile, disposable, polystyrene petri dish, 100 × 15 mm For sterile swabs we recommend: 70-3032 Cotton-tipped sterile applicators (10 packages of 2 applicators each) 70-3033 Cotton-tipped sterile applicators (1 box of 100 packages) For wire loops we recommend: 70-3060 Nichrome wire inoculating loop Tips for Successful Cultivation 1. Avoid contaminants. Do not use nonsterile plates, swabs, or media. 2.
To order call: 1-800-334-5551 (US and Canada) 336-584-0381 (International) For technical help call: 1-800-227-1150 www.carolina.
