SomaticView Version 1.
This page was intentionally left blank
SomaticView ™ A part of the NucleoCounter ® SCC-100™system Manual No. 991-0201 (English) Version 1.0 March 25, 2004 ChemoMetec A/S Gydevang 43, DK-3450 Allerød Telephone: +45 48 13 10 20, Fax: +45 48 13 10 21 Internet: www.chemometec.com, E-mail: info@nucleocounter.dk or info@chemometec.
This page was intentionally left blank ii
Customer support If you have questions about installing or using your SomaticView TM software check this user's guide first - you will find answers to most of your questions here. If you need further assistance, please contact customer support at ChemoMetec A/S.
This page was intentionally left blank iv
Intended use Intended use The SomaticView™ software is intended to be used with the NucleoCounter ® SCC-100™ instrument, for the purpose of counting of Somatic Cells in milk; it is intuitive and easy to use. SomaticView™ offers the user several features when connected to the NucleoCounter ® SCC-100™. SomaticView™ is intended for the documentation of results from the NucleoCounter ® SCC-100™, results can be printed or saved for later use using the SomaticView application.
Intended use This page was intentionally left blank vi
Table of contents Table of contents 1 2 3 4 5 6 7 INSTALLATION ................................................................................................... 1 1.1 I NSTALLATION OF THE USB DRIVER - W INDOWS 2000 ...................................................... 1 1.2 I NSTALLATION OF S OMATIC V IEW TM – W INDOWS 2000....................................................... 4 1.3 R EMOVAL OF THE S OMATIC V IEW SOFTWARE .....................................................................
Table of contents This page was intentionally left blank viii
1 Installation 1 Installation When the NucleoCounter SCC-100 is connected to a computer running SomaticView it is possible to register the estimated cell concentration and the collected image of the Cassette chamber. Connecting a NucleoCounter SCC-100 to a computer does not affect the operation of the NucleoCounter SCC-100 in any way and the SomaticView software performs no additional image analysis.
1 Installation Figure 1 The Found New Hardware Wizard. The driver needed is supplied on the NucleoCounter SCC-100 CD-ROM. Insert the CD-ROM and click “Next”. The next dialogue is shown on figure 2. Figure 2 Search for a driver for the NucleoCounter SCC-100. Select the default method “Search for a suitable driver for my device” and click “Next” to continue. The dialogue shown in figure 3 will appear.
1 Installation Figure 3 Locations where the computer searches for the driver for the NucleoCounter SCC-100. Make sure that the “CD-ROM drives” is selected as “Optional search location” and click “Next” to continue. Figure 4 The driver has been found on the NucleoCounter SCC-100 CD-ROM. Verify that the wizard has found “nucleo.inf” on the NucleoCounter SCC-100 CD-ROM and click “Next” to continue, see figure 4. If “nucleo.
1 Installation Figure 5 The installation has been successful. If the installation was successful the dialogue in figure 5 will appear. Click “Finish” to complete the installation. Now the USB device driver for the NucleoCounter SCC-100 is successfully installed. It will not be necessary to redo the installation unless you wish to use the NucleoCounter SCC100 on another computer or if you reinstall Windows 2000. 1.
1 Installation For the installation of SomaticView insert the NucleoCounter SCC-100 CD-ROM into the computer. To start the installation, locate the file “SETUP.EXE” on the CD-ROM and open it (double-click it using the mouse). The dialogue shown in figure 6 will appear. Click “Finish” to complete the installation. Figure 6 Dialogue box for installation of SomaticView.
1 Installation In the next dialogue Figure 8, please confirm the default destination folder for installation of the LabVIEW Run-Time Engine by clicking “Next”. Figure 8 Dialogue for the selection of the destination folder. Please select the default folder by clicking “Next” The dialogue shown in figure 9 finally allows you to implement a modification of the LabVIEW application. Click “Next” to continue or click “Back” to check your selection for the installation.
1 Installation If the installation is successful the dialogue shown in figure 10 will appear. Click “Finish” to complete the installation. The SomaticView software and LabVIEW application are now installed. Figure 10 Successful installation of the LabView application. For directions on start-up and use of the SomaticView software please refer to chapter 2 Getting started with SomaticView TM on page 11. 1.
1 Installation Figure 11 “System Properties” dialogue. In the “System Properties” dialogue select the “Hardware” tag (figure 11) and click on the “Device Manager” button. A list of system devices will be shown, see figure 12. Locate “nucleo Device” and open it by clicking on the to the left of the name. This will show its content, which also has the name “nucleo Device”.
1 Installation Highlight the “nucleo Device” as shown in figure 12 and option-click it using the right mouse button. This activates a pop-up menu. Select the “Uninstall…” function and the USB device driver will be removed from the system. 1.3.2 Removal of SomaticView TM - Windows 2000 To remove the SomaticView software 1, open the SomaticView folder (Figure 13).
1 Installation This page was intentionally left blank 10
2 Getting started with SomaticView TM 2 Getting started with SomaticView T M SomaticView allows the user to register the results of counting of Somatic cells in milk samples with the NucleoCounter SCC-100 on a computer. This is useful when documentation of results and data is needed. 2.1 Launch SomaticView TM SomaticView can be activated through the Windows “Start” menu. First select “SomaticView” from the “Programs” submenu, and then select “SomaticView”.
2 Getting started with SomaticView TM This page was intentionally left blank 12
3 The structure of SomaticView TM 3 The structure of SomaticView T M SomaticView is operated through a number of tabs. The different tabs are selected by clicking on the corresponding tab. The tabs are shown in figure 15. Figure 15 The three tabs of SomaticView. The default startup screen is the Analysis tab. For detailed descriptions of the different tabs see the following chapters. An overview of the three different tabs is given below. 3.
4 Analysis tab 4 Analysis tab Analysis is the default start-up tab of SomaticView. In this tab data and results transferred from the NucleoCounter SCC-100 are automatically displayed. An example of the Analysis tab is shown in figure 16. Once SomaticView has been launched the Analysis tab is automatically displayed. Usually there are no files in the active session upon start-up. Start the measurements or use the Browse button to navigate to another session (see chapter 4.2 Folder selection.).
4 Analysis tab 4.2 Folder selection. Each day SomaticView is launched, a new session (folder) is created. The folder’s name is the date of the session (yymmdd). It is possible to browse sessions created at an earlier date using the Folder button (Figure 17) Figure 17 The Folder button and selected folder. When a measurement is performed and data is transferred to the computer, a file containing the measurement data is created.
4 Analysis tab 3. Click “Select Cur Dir” and the session will be displayed in SomaticView. The files will appear in the file list 2 and it is possible to browse through them using the mouse. The selection of a session from a previous date does not affect the transmission of data from the NucleoCounter SCC-100.
4 Analysis tab Figure 19 SomaticView presents the images from the NucleoCounter SCC-100 like a starry sky. Each “star” represents a stained nucleus.
4 Analysis tab This page was intentionally left blank 18
5 Utility tab 5 Utility tab In the Utility tab (figure 20) it is possible to print and save results. Furthermore it is possible to select language, text editor and sound scheme. Figure 20 The Utility tab. 5.1 Print results The report path is found in the top of the Utility tab. Please note that printing only can take place if a valid printer is configured in the Printers folder of the Windows 2000 Control Panel.
5 Utility tab If no folder is selected SomaticView suggest that the folder from today is selected. Figure 22 Accept selection of today's folder Using the button to the right in Figure 21 any folder can be selected as the basis of a report. Once activated, locate the folder and click “Select Cur Dir”. The file that is printed is also saved on the hard disk of the PC (see Figure 23). If a report has already been created and stored, the new report will overwrite the older report.
5 Utility tab ChemoMetec A/S will implement new languages. Please contact ChemoMetec A/S NucleoCounter SCC-100 Technical Support on the address info@nucleocounter.com for any update on available languages. 5.3 Select sound scheme Figure 25 Select a sound scheme Using this list box you can set a sound to appear whenever a new result is present in SomaticView. Every time SomaticView is started the "Windows ding sound" is enabled. 5.
5 Utility tab Either WordPad or Notepad 3 can be configured as text editors. Therefore, if it is not possible to locate “wordpad.exe”, then: a. Select Cancel from Figure 28, press OK when the message box "Printing Report" appears. b. Then select Notepad from the text editor list box and press the Print button again. Figure 28 This dialogue appears when WordPad is configured as text editor. 5.4.
5 Utility tab 2. Choose the location to save the file to be the SomaticView folder (usually C:\SomaticView). 3. In the bottom of the dialogue change the “Save as type:” to “Text Document”. Make sure there is a checkmark in the check box to the left of “Save in this format by default”. If there is no checkmark present set it using the mouse. 4. Change the file name to “Document.txt”. 5. Click “Save”. Figure 29 The dialogue shown when setting up the font for WordPad. 6.
5 Utility tab 7. Exit WordPad. 5.4.2 Define font for Notepad Similarly Notepad can be set up for SomaticView, follow the directions below. 1. Open Notepad. 2. Choose “Font…” through the Format menu. The dialogue is shown in figure 31. Figure 31 Defining the font for Notepad. 3. Choose the font Courier, Font style Regular and Size 10 as in figure 31. Now Press OK and exit Notepad. Notepad will use the font Courier, size 10 regular.
6 Troubleshooting 6 Troubleshooting Occasionally difficulties with the SomaticView software may occur. In the following a short description and suggested action is given. 6.1 Quality of the sample Through the CM file image it is possible visually to assess the quality of the sample preparation and thereby the reliability of the measurement by looking at the “starry sky”. The measurement is reliable if the stars or white dots are distributed separately and evenly over the image.
6 Troubleshooting Figure 33 Input open failure Figure 3 4 Input opened If "Input open failure" is stated in the Info field, check the USB cable. Alternative, if the NucleoCounter SCC-100 is turned off, “Input open failure” will also appear in the Info field. If data still is not received quit SomaticView and close the NC Receiver. Then open SomaticView and check that the NC Receiver is also started. If necessary, restart the computer before opening SomaticView again.
6 Troubleshooting SomaticView is activated. If the “QUIT”-button does not respond (after only few seconds), you can close SomaticView down using the Windows Task Manager. For instructions on the use of Windows Task Manager please refer to the documentation supplied with Windows. Low resources on the PC running SomaticView can cause the result field to show the following message "Not SCC-100". You can check your resources on the PC using the Windows Task Manager.
6 Troubleshooting This page was intentionally left blank 28
7 Technical specifications 7 Technical specifications 7.1 SomaticView™ software System requirements For operating systems see table 1 below. USB 1.1 port 5. Display Screen Area: Minimum 1024 x 768 6. Before installation of SomaticView the USB driver for the NucleoCounter SCC-100 must be installed (see chapter 1.1 Installation of the USB driver - Windows 2000). Table 1 Functionality of SomaticView and the USB driver on computers running different operating systems.
7 Technical specifications This page was intentionally left blank 30
8 Appendix 1: Printed report list 8 Appendix 1: Printed report list Below is an example of what the printed and saved report list looks like when it is generated. The file can easily be exported to e.g. EXCEL. C:\Somaticview\data files\040114\Todays report 040114.txt Date: 14-01-2004 Time: 13:09 NucleoCounter SCC-100: 004-12 Filename 040114-001.CM 040114-002.CM 040114-003.CM SCC/ml 98,000 737,000 1137,000 The header contains: • The path for the report file.
8 Appendix 1: Printed report list This page was intentionally left blank 32
9 Index A Analysis tab ............................................14 B Browse files from active session ...........15 N NC Receiver............................................ 11 nucleo Device .......................................... 8 nucleo.inf ................................................. 3 Browse stored files.................................15 O C Operating systems ................................. 29 Collected image ......................................16 P Connecting USB cable ......
