Datasheet

Getting Started 5 Tour through the Clarity station
Fig 17: Calibration window - loaded standard
l Set the calibration level number to 1 on the Current Level field . Use the
Add All icon (blue) to move all identified peaks to the calibration
table. The Calibration table appears in the Calibration window, ready to
be completed as seen on Fig 17 on pg 28.
l As it can be seen in the calibration, individual peaks are now identified
according to their retention times only. Click and edit the fields in the
Compound Name column to those seen on Fig 17 on pg 28. You may
also set the peak color for some peak, for example the ISTD peak, in the
Peak Color column.
l Fill the Amount column with the concentration of the particular
compounds. In this standard mixture, all compounds except for the peak
number 6 have concentration of 0.4.
l Peak number 6 is marked as ISTD peak. In the Is ISTD column, change its
type to ISTD1 and then set its amount in the Amount column to 2.
l The first calibration level is now set. On the tabs of the individual
compounds (named according to the Compound Name field) the graph
with single-point linear calibration can be seen.
l Proceed to setting the other calibration levels. The operation is quite
simple - use the Open Standard icon (yellow) again to open another
calibration standard named STD 2.PRM. Set the calibration level in the
Current Level field to 2 and use the Add All icon (blue) . Fill in the
Amount column with 1.0 values (except for the peak 6, in which the 2
value should be used again).
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