Datasheet

5 Tour through the Clarity station Clarity
l Set the third calibration level accordingly using the STD 3.PRM file and
Amount of 3.0 and the fourth level (file STD 4.PRM, Amount 5.0) except for
the ISTD peak (always Amount = 2 ). On the tabs of the individual
compounds , the linear four-point calibration can be seen. Save the
calibration file now using the Save Calibration icon under the name
CALIBDEMO.CAL into the default directory.
Note: All the steps from the beginning of this chapter would be performed
automatically during the measurement of the sequence shown in the
chapter "Sequence window" on pg 24.
5.2.3 Linking the calibration to a chromatogram
l Any chromatogram can be linked to a calibration file, thus automatically
giving calibrated results. In the Instrument window, use the Chromatogram
icon to open the Chromatogram window.
l
Use the Open Chromatogram icon to open chromatogram data based
on the calibration you just created. Use the SAMPLE_VIAL_6-1.PRM file
saved in the default directory. Other files in the directory are uncalibrated
too, but they will be used later.
l The data are uncalibrated and no information about the names of
individual compounds is available, peaks in the Result Table are just
described according to their retention times. To change this, the
appropriate calibration should be linked to these data.
l Select the Results tab (it should be opened automatically) and look at the
section on the right side of the screen. Use the Set... button in the
Calibration File (Peak Table) section to select the calibration file created
in the previous chapter (it should be in the default directory under the
name CALIBDEMO.CAL . Any peaks present in calibration are now
identified with their names. Check the Calculation field to the right - it
should list ISTD. If it does not, change it to ISTD.
Note: In case you skipped the process of making your own calibration, used the
DEMO1.CAL file instead of CALIBDEMO.CAL.
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