Specifications

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9. Instrument: On the LC-20AD liquid chromatograph, open the drain by turning the knob
anti-clockwise, and press purge. When purging is complete, close the drain by turning the
knob clockwise
10. Instrument: Press pump to turn on the pump and allow for pressure to stabilize and record
the final pressure (allow solvent flow for about ~ 10 min and monitor pressure)
Setting parameters for the analysis
11. Software: Click on Data Acquisition tab and set parameters such as LC stop time, Pump
mode, Solvent compositions, Detector wavelengths, Oven temperature etc. Save method
by clicking on File>Save method file as>
Sample preparation and using the autosampler
12. Instrument: Filter sample through a Nylon 0.45 micron membrane, place sample in blue-
capped Prominence vial (minimum sample level has to be above the middle white marker
on the vial), insert samples in the sample tray by opening the autosampler door (left
bottom) and pulling out the tray—note the vial position number of each sample on the
tray. Reposition the tray by pushing it in—make sure it clicks in, and close the
autosampler door
Starting the analysis
13. Software: Click on Batch Table tab and enter sample information—Vial number (from
the autosampler tray), Sample name, Injection volume…etc. To choose the previously