Specifications

14
created method file, click on the black arrow at the right corner of the box on the table
and load the already saved method file
14. Software: Once the table is complete, save batch file by clicking on File>Save batch file
as
15. Software: Click on batch start (green triangle on the left)
16. During the analysis make sure that the solvent levels do not run below ~300 mL and that
the waste bottle does not overflow
17. Monitor pressure carefully and note any changes in the column pressure during the
analysis—record final pressure after the analysis is complete
Switching off the instrument
18. Software: Once the analysis is complete, stop the flow by changing Pump A. Flow to
0.00 mL/ min
19. Software: Exit software
20. Instrument: Turn off the communicator bus module by pressing the power button
21. Instrument: Turn off everything else (LC-20AD liquid chromatograph, SIL-20A
autosampler, RID-10A by refractive index detector, SPD-20AV UV detector and CTO-
20AC column oven) by pressing the power buttons