User manual

8 56-3124-15 Edition AF 2003-08
ÄKTAdesign systems
Access to improvements in UNICORN 3.21:
The items listed below are dependent upon the system strategy and cannot be utilized if
an earlier system strategy is used.
New features
• When adding new components to a system, instructions are shown only for the
specific component selected
• Interactive flow scheme
• Interactive run data
• Hold_ Until instruction
Action: New standard ÄKTAdesign system strategies are being updated and supplied on
UNICORN CD-ROM as soon as they are fully tested. Please contact your local
Amersham Biosciences representative if you do not have the latest strategy.
Installation test ÄKTAbasic 10
There is an error in the automatic evaluation of the installation test for strategy
B10F300. The Step Response will not be evaluated correctly and the result will be Step
Response Rejected.
Action: Perform a manual evaluation of the Step Response according to the Basic user
manual or use the template from B10_300.
Differing BufferPrep recipes
If you open a template from strategy version 1.xx, you may get a message: “Method
recipe is different from corresponding Global / Personal recipe. Update method recipe?”
Action: Answer Yes and save the template as your method or as a new template. Do not
use the old template.
You may also update the strategy to version 2.xx (available during the autumn).
Columns with 0.3 MPa pressure limit
Columns with a 0.3 MPa pressure limit in their data file have a pressure limit of 0.5 MPa
in the UNICORN column list, to enable running them also in ÄKTA systems with high
system pressure.
Action: Change the pressure limit of the column to 0.3 MPa if your ÄKTA system has a
low (under 0.2 MPa) system pressure.
System calibration
When pressure has been calibrated and the flow re-started the pressure values on screen
in System Control show zero pressure.
Action: Press End. Restart Flow.